General information for g15894.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Leukotriene A-4 hydrolase.
Parent gene
- The parent gene g15894 is located on chr_4: 7201690..7203778 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15894 | g15894.t5 | TTS | g15894.t5 | 7199965 | 7199965 |
| chr_4 | g15894 | g15894.t5 | isoform | g15894.t5 | 7200561 | 7203778 |
| chr_4 | g15894 | g15894.t5 | exon | g15894.t5.exon1 | 7200561 | 7200963 |
| chr_4 | g15894 | g15894.t5 | cds | g15894.t5.CDS1 | 7200561 | 7200963 |
| chr_4 | g15894 | g15894.t5 | exon | g15894.t5.exon2 | 7201453 | 7201591 |
| chr_4 | g15894 | g15894.t5 | cds | g15894.t5.CDS2 | 7201453 | 7201591 |
| chr_4 | g15894 | g15894.t5 | exon | g15894.t5.exon3 | 7201659 | 7202292 |
| chr_4 | g15894 | g15894.t5 | cds | g15894.t5.CDS3 | 7201659 | 7202292 |
| chr_4 | g15894 | g15894.t5 | exon | g15894.t5.exon4 | 7202348 | 7202515 |
| chr_4 | g15894 | g15894.t5 | cds | g15894.t5.CDS4 | 7202348 | 7202506 |
| chr_4 | g15894 | g15894.t5 | exon | g15894.t5.exon5 | 7203766 | 7203778 |
| chr_4 | g15894 | g15894.t5 | TSS | g15894.t5 | 7203793 | 7203793 |
Sequences
>g15894.t5 Gene=g15894 Length=1357
TCTAATAAATTAGACTTCAAACATGCCACGTCTTGGAACAGATCCATCATCTTATTCTCA
ACCTGATCTTGTAAAGATCAAACATATTGATTTGGACTGGACTGTTGATTTTAGTCAGAA
AATTATCAGTGGAAGTGCTCAAATTCAATTCATCATTTTAACGAAATTCATTGAGGAAAT
TCTTCTTGATGTCAGTGATATAACAATTGATTCTGTTAATGTTCTCAATGAAGGCTGTGA
AATTCCTTTGAAATTTGATATTTCTGGATTGGTTCCTGATATTGGTTCAAAACTTACAAT
TACTTTGCCAACAAAAACTGATGGCAAATTGACCTTGAAAATTTACTACAAAACATCCAG
CAGTGCAAGCGCTTTACAATGGCTCACACCTGAACAAACTCTTGGCAAAAAACATCCATT
CATGTTCAGTCAATGTCAAGCAATTCATGCTCGAAGTATTCTTCCATGTCAAGACTCACC
ATCAGTTAAATTCACTTACAATGCGACACTTAGACATTCACCAGAATTGACTGGTTTAAT
GAGTGCAATTCGTCAAGGAAGTGAAAATGGTGTAACCAAATATGTGCAAAAAGTTCCAAT
TCCAAGTTATTTGCTTGCTATTGCAGTTGGTGATTTGTATTCGAAAAAAATTGGACCAAG
ATCACATGTATGGGCTGAAAAAGACATTGTTGATGAAGCTGCTGAGGAATATAGTGACAC
TGAAAAGTTTTTGAAAACAGCTGAAGATATTTGCGGACCTTATTTATGGCAGCAATATGA
TTTGCTTGTTATGCCACCTTCTTTCCCATTTGGAGGCATGGAAAATCCATCATTGACTTT
TATCACTCCTACAACAATTGCTGGTGATAAATCACTTGTAGATGTTGTTGCACATGAAAT
TTCTCATTCATGGACTGGAAATCTTGTAACAAATGCAAACTTTGAACATTTCTGGCTCAA
TGAAGGTTTCACAGTATTTGTCGAACAAAAAATCATCGGACGTCTTCGTGGTGATGAATA
TCGTGATTTCCATGCCCTTCACGGTCTCTCAGACTTGAAAGACACGATCAAAAATCAATT
GAAAAATGAACCAGAATTAACAAAACTTGTTGTTGATTTGAGCAATGTTGGTCCAGATGA
TGCTTTTAGTTCTGTTCCATATATGAAAGGAAGCACATTCTTGAGATATTTGGAAGATCT
TTTCGGTGGTCCAGGAATTTTTGAGCCATTTTTGAGATCGTATTTGGATCAATTTAAATA
TCAATCAATTTTGACAAAAGATTTTAAGAAATATTTGTATGATTATTTCAAAGATAAAAT
TCCTGAAAAATTGGAACAAGTTGATTGGGATAAATGG
>g15894.t5 Gene=g15894 Length=445
MPRLGTDPSSYSQPDLVKIKHIDLDWTVDFSQKIISGSAQIQFIILTKFIEEILLDVSDI
TIDSVNVLNEGCEIPLKFDISGLVPDIGSKLTITLPTKTDGKLTLKIYYKTSSSASALQW
LTPEQTLGKKHPFMFSQCQAIHARSILPCQDSPSVKFTYNATLRHSPELTGLMSAIRQGS
ENGVTKYVQKVPIPSYLLAIAVGDLYSKKIGPRSHVWAEKDIVDEAAEEYSDTEKFLKTA
EDICGPYLWQQYDLLVMPPSFPFGGMENPSLTFITPTTIAGDKSLVDVVAHEISHSWTGN
LVTNANFEHFWLNEGFTVFVEQKIIGRLRGDEYRDFHALHGLSDLKDTIKNQLKNEPELT
KLVVDLSNVGPDDAFSSVPYMKGSTFLRYLEDLFGGPGIFEPFLRSYLDQFKYQSILTKD
FKKYLYDYFKDKIPEKLEQVDWDKW
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g15894.t5 | CDD | cd09599 | M1_LTA4H | 7 | 445 | 0 |
| 12 | g15894.t5 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 16 | 203 | 0 |
| 14 | g15894.t5 | Gene3D | G3DSA:1.10.1740.60 | - | 204 | 300 | 0 |
| 13 | g15894.t5 | Gene3D | G3DSA:1.10.390.10 | Neutral Protease Domain 2 | 301 | 445 | 0 |
| 3 | g15894.t5 | PANTHER | PTHR45726 | - | 6 | 445 | 0 |
| 4 | g15894.t5 | PANTHER | PTHR45726:SF3 | LEUKOTRIENE A-4 HYDROLASE | 6 | 445 | 0 |
| 5 | g15894.t5 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 144 | 159 | 0 |
| 6 | g15894.t5 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 187 | 202 | 0 |
| 7 | g15894.t5 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 261 | 271 | 0 |
| 8 | g15894.t5 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 288 | 303 | 0 |
| 9 | g15894.t5 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 307 | 319 | 0 |
| 2 | g15894.t5 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 21 | 197 | 0 |
| 1 | g15894.t5 | Pfam | PF01433 | Peptidase family M1 domain | 235 | 445 | 0 |
| 11 | g15894.t5 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 4 | 204 | 0 |
| 10 | g15894.t5 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 209 | 445 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K01254 (leukotriene-A4 hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
