General information for g15907.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Membrane-bound transcription factor site-2 protease.
Parent gene
- The parent gene g15907 is located on chr_4: 7361625..7363221 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15907 | g15907.t1 | TTS | g15907.t1 | 7361584 | 7361584 |
| chr_4 | g15907 | g15907.t1 | isoform | g15907.t1 | 7361625 | 7363221 |
| chr_4 | g15907 | g15907.t1 | exon | g15907.t1.exon1 | 7361625 | 7362655 |
| chr_4 | g15907 | g15907.t1 | cds | g15907.t1.CDS1 | 7361625 | 7362655 |
| chr_4 | g15907 | g15907.t1 | exon | g15907.t1.exon2 | 7362753 | 7363221 |
| chr_4 | g15907 | g15907.t1 | cds | g15907.t1.CDS2 | 7362753 | 7363221 |
| chr_4 | g15907 | g15907.t1 | TSS | g15907.t1 | 7363287 | 7363287 |
Sequences
>g15907.t1 Gene=g15907 Length=1500
ATGGATTTGACAATTTTAGTTTTAATTTGTATAATTTATGCAATTGTGATGTTTATTGAT
TTTTTCTTTAAAAGCTGCATGATGCTGCCATATCTTGATTTTCTAAGCAATACAGGCATC
AGTGTCAAGTTTTTCAGTATAAAATTTCATACAAATGCATTTAATCGATTTATTTCACGG
CATAGCTCAAAATTACCAAATATATATAAAAATTCTTTTAAATTTGGCTTTTACATCACA
ATATTATTACTACCGATCTCATTAATTGTGATGATTCTTTCACTTTTCTCTGGATCATCA
TCAGATACAATAAAAAATCAAAATACGAATTTAATAAGTGATAATAAAAATGATGAAAGT
GGAAATGGAGCACATTTGGAAATTTTATTACCTGGTGTTAATTTGCCATTGGATCAAATT
GGATATTATGTGATATCATTGCTAATTTGCTCTGTTGTTCATGAATCAGGTCATGGAATA
GCAGCAGTTTTAGAAGACATACCAGTTTCTGGATTTGGTCTTAATTTAATTTTCATTCTA
CCAATTGCATACACACAAATTGACACAGATCATTTACAAACAGCTCGTTTATGGAAGAAA
TTAAAAATTTTCTCAGCAGGAATTTGGAATAATTTAATACTCGCTGGATGGTGCTATATT
TTGTTACTTTTACTTCCAATAGTTTTATTACCTATTTTTAATTCTGGCAATGCAGTTTTC
ATCACAAAAATTAAACAAGGAGCGCCAATCAAAGGTGAAAATGGCCTTTATGTTGGTGAT
TCAGTTTATAGCATAAATGACTGTAAAGTAAGCAATGAAGATGAATGGTTGAAATGTTTG
ACTTTATCACTTAAAATACATCCAGCATATTGTGTCAGTGAAGATTTTATTCATGAAAAT
GATGAATCAGTGCATGAAATTGAACATCAAAAAGATGGCACAATTGAATGTTGTAGTAAA
TCAAATCCAGCAGTGAATTGTTTTGAGAATTTTGATGAAGAACGACTACCGCAATATGTT
TGTCTAAATATACGTAATGCAATTGAACATTCCAAGGCTTATTGTCAAGAATCATTGACT
TGCACTGAACACATTTCATGTGTAAAACCATTGTTGGCTAATACATCCACAATCATTCAC
ATAAAGCGAAGAAATCGAGTAAAAGATTTTGTTTATTATGGACATCCAATTGACATAATT
GGAAGCGTTGAAATTTCACAGTTTATACCAAAAACAAAAACTTTTAGTCCAGCATTTGCT
GATTCAATTGCACTTTTGTTAAAATATCTTGTCGTTTTTTCATCTGGACTTGCAATAGTC
AATGTTATTCCATGTTATGGACTTGATGGACAGCATTTAATTAATGCAATTATCTCAAAT
TTACCGTCGAAGTATTTTAGTAAAGGCAGAAAGGAAGTTTTGTCAATATCAATTAATTTA
GTTGGAAGTTTAACATTAATAACAATTATTATTAAAATTTTATGGACGACTTTTTTGTAA
>g15907.t1 Gene=g15907 Length=499
MDLTILVLICIIYAIVMFIDFFFKSCMMLPYLDFLSNTGISVKFFSIKFHTNAFNRFISR
HSSKLPNIYKNSFKFGFYITILLLPISLIVMILSLFSGSSSDTIKNQNTNLISDNKNDES
GNGAHLEILLPGVNLPLDQIGYYVISLLICSVVHESGHGIAAVLEDIPVSGFGLNLIFIL
PIAYTQIDTDHLQTARLWKKLKIFSAGIWNNLILAGWCYILLLLLPIVLLPIFNSGNAVF
ITKIKQGAPIKGENGLYVGDSVYSINDCKVSNEDEWLKCLTLSLKIHPAYCVSEDFIHEN
DESVHEIEHQKDGTIECCSKSNPAVNCFENFDEERLPQYVCLNIRNAIEHSKAYCQESLT
CTEHISCVKPLLANTSTIIHIKRRNRVKDFVYYGHPIDIIGSVEISQFIPKTKTFSPAFA
DSIALLLKYLVVFSSGLAIVNVIPCYGLDGQHLINAIISNLPSKYFSKGRKEVLSISINL
VGSLTLITIIIKILWTTFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g15907.t1 | PANTHER | PTHR13325 | PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE | 6 | 497 | 5.0E-100 |
| 5 | g15907.t1 | PRINTS | PR01000 | Sterol regulatory element binding protein site 2 protease (M50) signature | 128 | 147 | 3.1E-13 |
| 6 | g15907.t1 | PRINTS | PR01000 | Sterol regulatory element binding protein site 2 protease (M50) signature | 151 | 165 | 3.1E-13 |
| 4 | g15907.t1 | PRINTS | PR01000 | Sterol regulatory element binding protein site 2 protease (M50) signature | 167 | 182 | 3.1E-13 |
| 3 | g15907.t1 | PRINTS | PR01000 | Sterol regulatory element binding protein site 2 protease (M50) signature | 183 | 195 | 3.1E-13 |
| 1 | g15907.t1 | Pfam | PF02163 | Peptidase family M50 | 143 | 475 | 1.5E-21 |
| 18 | g15907.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 27 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 23 | - |
| 13 | g15907.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 24 | 74 | - |
| 23 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 75 | 96 | - |
| 17 | g15907.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 97 | 139 | - |
| 24 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 140 | 157 | - |
| 12 | g15907.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 158 | 168 | - |
| 21 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 169 | 187 | - |
| 16 | g15907.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 188 | 206 | - |
| 22 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 207 | 233 | - |
| 15 | g15907.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 234 | 389 | - |
| 28 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 390 | 409 | - |
| 20 | g15907.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 410 | 428 | - |
| 25 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 429 | 448 | - |
| 14 | g15907.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 449 | 472 | - |
| 26 | g15907.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 473 | 495 | - |
| 19 | g15907.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 496 | 499 | - |
| 8 | g15907.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 23 | - |
| 7 | g15907.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 75 | 97 | - |
| 9 | g15907.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 208 | 230 | - |
| 11 | g15907.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 423 | 445 | - |
| 10 | g15907.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 473 | 495 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006508 | proteolysis | BP |
| GO:0004222 | metalloendopeptidase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K07765 (S2P endopeptidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
