Gene loci information

Transcript annotation

  • This transcript has been annotated as Pyruvate kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g160 g160.t14 TSS g160.t14 1440552 1440552
chr_3 g160 g160.t14 isoform g160.t14 1440565 1445966
chr_3 g160 g160.t14 exon g160.t14.exon1 1440565 1440694
chr_3 g160 g160.t14 exon g160.t14.exon2 1443302 1443971
chr_3 g160 g160.t14 cds g160.t14.CDS1 1443302 1443971
chr_3 g160 g160.t14 exon g160.t14.exon3 1444043 1444756
chr_3 g160 g160.t14 cds g160.t14.CDS2 1444043 1444756
chr_3 g160 g160.t14 exon g160.t14.exon4 1444811 1444967
chr_3 g160 g160.t14 cds g160.t14.CDS3 1444811 1444967
chr_3 g160 g160.t14 exon g160.t14.exon5 1445263 1445966
chr_3 g160 g160.t14 cds g160.t14.CDS4 1445263 1445275
chr_3 g160 g160.t14 TTS g160.t14 NA NA

Sequences

>g160.t14 Gene=g160 Length=2375
CTCTCGATTTAGCCGGACATTAGTCAGTGAAAATTAAATTAATTTGTGATAAATACTGTT
CTGTTCATTAAAAATTGAACCAATCTGTCATTTATTCGAGTTATTTATTTAAATTAAATA
AGAAATCAAGATGAGCGAAAAATTGCCACAAATGAAGGCCGCTGAAGCAATCTCTGCATT
GGACCATCTCTGTCAATTGGATCCAAATGCAAAAACAGGATTTATTCGTTTAACTGGATT
GATTTGTACTATCGGACCAGCTTCACGATCACCCGAAATGTTGGAGAAAATGATGCATTG
TGGCATGAACATTGCTCGTATGAATTTCTCACATGGAACTCATGAATATCATGCTGGAAC
TATTAAGAATGTTCGTGAGGCTGTCGATAATTATTCGAAAAAATTGGGCTATAAGTATGC
TCTTGCAATAGCATTGGATACAAAAGGTCCCGAAATCCGTACTGGTTTGAATGCTGATAA
GGGTGATATTTCATTGTCTCGTGGTGATAAGATTAAAGTTACAACTAATAAGGAATGGTT
TGAAAAGGGATCTAAGGAACAAATCTATGCTGATTATCCAAACATGGTTAAAGTTTTGAA
AACAGGTGATCGTATCTTCATTGATGATGGTTTGATCTCACTCGTTGTCGATTCAATTAA
TGGCGATACATTGGACTGCACAATTGAAAATGGTGGTAAATTAGGCCAACAAAAGGGTGT
GAATCTTCCAAATGTACCAGTTGATTTACCAGCTGTTAGTGAGAAAGACAAGTCAGATTT
ACTTTTTGGTGTTGAACAAGATATGATTTTTGCTTCATTCATTCGTGATGGACATGCATT
GGATGAAATTCGTAAAATTTTGGGTGAGGCTGGCAAGAACATTAAAATCATCTCAAAAAT
TGAGAATCAACAAGGTTTACAAAATATTGATGAAATTATTGAGAAATCTGATGGTATTAT
GGTTGCTCGTGGCGATTTGGGTATTGAAATTCCACCAGAAAAGGTTTTCCTTGCTCAAAA
ACAAATTACTGCTCGCTGCAATCGCGCTGGAAAACCATGTATTTGCGCTACTCAAATGTT
GGAGTCAATGACAAACAAGCCACGTGCTACACGCGCTGAAATCTCAGACGTTGCCAATGC
CGTCTTGGATGGTGCTGATTGCACAATGTTGTCAGGAGAAACTGCTAAGGGCGAGTATCC
ACTCGAATGTATCACAACTATGGCAAAAACACAAATGGAAGCTGAAGCTGCTATTTGGCA
CAAGAATTTATTCCGTGATTTGGTTTCATTGCAACCAACACCAATTGATGCTACACACTC
GATTGCTATCTCAGCTGTTGAAGCTGCATCAAAGACTCTTGCTTCATGCATCATTTGTCT
CACTACATCGGGAAGATCGGCACATTTGATCTCCAAATATCGCCCACGATGCCCAATCAT
TACAGTAACACGCTTTGAACAAACTGCACGCCAATGTCATCTTTTCCGCGGCATTTTGCC
ATTGATCTATCAAGATGCTGTTCAAGAAGACTGGTTGAAAGATGTTGATGCACGTGTCCA
ATTCGGTATCAAATTTGGAAAATCTCGTGGATTTATCAAGAGTGGTGATCCAATTGTCGT
TGTTACTGGCTGGAAGTCTGGATCTGGTTTTACAAACACCTTGCGCGTTTTGAACTGTGA
ATAAGCACATCATCATCGATCGTATTAACAAAAAAATTACATCATATATTACTACTACCT
ATAGCCGATGTCTCTTCTAATCATTGAATTAATTTCTCTGTTCCTTAAAGTGCTACATAT
ATGTAGGACAACATCGTTAAGATAGTGAAAGAAACCTAATAATTTTAATTAACTTCTAAA
GTGTTAACAAACACAAACCACCACCGTTAATAGCAACAGCAACTACAGATCATACAAGAA
GAACAGTAAAAATGAAAAGAAAGTGTGTAACTAATTTCTCATTAGAACAAAAATTCACAT
TAAAGCGACACAATACATACTATTTATTTCAATTTGTTCCCAATTATTACACACTGAAAA
GTTGATCTTAAAAGTTTTATGAAAAACATAATTGTATTTATGAACAGAAGATTCAAATTG
TTCTTAATGAATAATAATAAATGATGAAATTATCAATTATTGAAACGATATTTTTTTGTT
TTTATATTTTCAGGAATATGATACATTTTGATCATAAATTTGTGTGGTTGGCGAGTATTG
TTTGAGATCATTTTAGTTACTTTTTAAATAAATTGAAGACACAACTTGGTTTTCTATATG
AAAAATTTAATGCTGATGATTTTATTCATGTAGTTTAAGAATTTATGAGACAATAAAACT
AATTAATTTTGTAGATACAAGTGCAAATTTTCTAT

>g160.t14 Gene=g160 Length=517
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVEQDMIFASFIRDGHALDEI
RKILGEAGKNIKIISKIENQQGLQNIDEIIEKSDGIMVARGDLGIEIPPEKVFLAQKQIT
ARCNRAGKPCICATQMLESMTNKPRATRAEISDVANAVLDGADCTMLSGETAKGEYPLEC
ITTMAKTQMEAEAAIWHKNLFRDLVSLQPTPIDATHSIAISAVEAASKTLASCIICLTTS
GRSAHLISKYRPRCPIITVTRFEQTARQCHLFRGILPLIYQDAVQEDWLKDVDARVQFGI
KFGKSRGFIKSGDPIVVVTGWKSGSGFTNTLRVLNCE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
18 g160.t14 CDD cd00288 Pyruvate_Kinase 32 514 0.0
15 g160.t14 Gene3D G3DSA:3.40.1380.20 - 16 513 1.5E-232
17 g160.t14 Gene3D G3DSA:3.20.20.60 - 33 371 1.5E-232
16 g160.t14 Gene3D G3DSA:2.40.33.10 - 107 207 1.5E-232
3 g160.t14 PANTHER PTHR11817:SF100 PYRUVATE KINASE 10 517 3.1E-224
4 g160.t14 PANTHER PTHR11817 PYRUVATE KINASE 10 517 3.1E-224
8 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 96 112 4.6E-75
7 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 223 237 4.6E-75
5 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 253 279 4.6E-75
11 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 280 304 4.6E-75
6 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 305 329 4.6E-75
9 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 330 348 4.6E-75
10 g160.t14 PRINTS PR01050 Pyruvate kinase family signature 349 365 4.6E-75
2 g160.t14 Pfam PF00224 Pyruvate kinase, barrel domain 33 380 7.4E-151
1 g160.t14 Pfam PF02887 Pyruvate kinase, alpha/beta domain 397 514 7.2E-36
19 g160.t14 ProSitePatterns PS00110 Pyruvate kinase active site signature. 251 263 -
14 g160.t14 SUPERFAMILY SSF51621 Phosphoenolpyruvate/pyruvate domain 18 380 2.86E-97
12 g160.t14 SUPERFAMILY SSF50800 PK beta-barrel domain-like 107 206 1.64E-26
13 g160.t14 SUPERFAMILY SSF52935 PK C-terminal domain-like 386 516 7.72E-43
20 g160.t14 TIGRFAM TIGR01064 pyruv_kin: pyruvate kinase 34 514 6.6E-175

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0030955 potassium ion binding MF
GO:0000287 magnesium ion binding MF
GO:0004743 pyruvate kinase activity MF
GO:0006096 glycolytic process BP
GO:0003824 catalytic activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed