Gene loci information

Transcript annotation

  • This transcript has been annotated as Pyruvate kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g160 g160.t2 isoform g160.t2 1439399 1444756
chr_3 g160 g160.t2 exon g160.t2.exon1 1439399 1439547
chr_3 g160 g160.t2 exon g160.t2.exon2 1443302 1443971
chr_3 g160 g160.t2 cds g160.t2.CDS1 1443302 1443971
chr_3 g160 g160.t2 exon g160.t2.exon3 1444037 1444756
chr_3 g160 g160.t2 cds g160.t2.CDS2 1444037 1444755
chr_3 g160 g160.t2 TTS g160.t2 1445733 1445733
chr_3 g160 g160.t2 TSS g160.t2 NA NA

Sequences

>g160.t2 Gene=g160 Length=1539
CTGATTTTCATAGAAAAGAACACCGACTAAGTGTGATTGTCAAAATAAGAATTTCATTCA
CAGTCTCATTTTGTCATTTAACCGGACAAGTTCAAATAGCAAGAGGCGAAAAGAACATTT
ATTTGCTTGTACTGTGTATTATATTGTGTATGAGCGAAAAATTGCCACAAATGAAGGCCG
CTGAAGCAATCTCTGCATTGGACCATCTCTGTCAATTGGATCCAAATGCAAAAACAGGAT
TTATTCGTTTAACTGGATTGATTTGTACTATCGGACCAGCTTCACGATCACCCGAAATGT
TGGAGAAAATGATGCATTGTGGCATGAACATTGCTCGTATGAATTTCTCACATGGAACTC
ATGAATATCATGCTGGAACTATTAAGAATGTTCGTGAGGCTGTCGATAATTATTCGAAAA
AATTGGGCTATAAGTATGCTCTTGCAATAGCATTGGATACAAAAGGTCCCGAAATCCGTA
CTGGTTTGAATGCTGATAAGGGTGATATTTCATTGTCTCGTGGTGATAAGATTAAAGTTA
CAACTAATAAGGAATGGTTTGAAAAGGGATCTAAGGAACAAATCTATGCTGATTATCCAA
ACATGGTTAAAGTTTTGAAAACAGGTGATCGTATCTTCATTGATGATGGTTTGATCTCAC
TCGTTGTCGATTCAATTAATGGCGATACATTGGACTGCACAATTGAAAATGGTGGTAAAT
TAGGCCAACAAAAGGGTGTGAATCTTCCAAATGTACCAGTTGATTTACCAGCTGTTAGTG
AGAAAGACAAGTCAGATTTACTTTTTGGTGTTGAACAAGGTGTTGATATGATTTTTGCTT
CATTCATTCGTGATGGACATGCATTGGATGAAATTCGTAAAATTTTGGGTGAGGCTGGCA
AGAACATTAAAATCATCTCAAAAATTGAGAATCAACAAGGTTTACAAAATATTGATGAAA
TTATTGAGAAATCTGATGGTATTATGGTTGCTCGTGGCGATTTGGGTATTGAAATTCCAC
CAGAAAAGGTTTTCCTTGCTCAAAAACAAATTACTGCTCGCTGCAATCGCGCTGGAAAAC
CATGTATTTGCGCTACTCAAATGTTGGAGTCAATGACAAACAAGCCACGTGCTACACGCG
CTGAAATCTCAGACGTTGCCAATGCCGTCTTGGATGGTGCTGATTGCACAATGTTGTCAG
GAGAAACTGCTAAGGGCGAGTATCCACTCGAATGTATCACAACTATGGCAAAAACACAAA
TGGAAGCTGAAGCTGCTATTTGGCACAAGAATTTATTCCGTGATTTGGTTTCATTGCAAC
CAACACCAATTGATGCTACACACTCGATTGCTATCTCAGCTGTTGAAGCTGCATCAAAGA
CTCTTGCTTCATGCATCATTTGTCTCACTACATCGGGAAGATCGGCACATTTGATCTCCA
AATATCGCCCACGATGCCCAATCATTACAGTAACACGCTTTGAACAAACTGCACGCCAAT
GTCATCTTTTCCGCGGCATTTTGCCATTGATCTATCAAG

>g160.t2 Gene=g160 Length=463
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVEQGVDMIFASFIRDGHALD
EIRKILGEAGKNIKIISKIENQQGLQNIDEIIEKSDGIMVARGDLGIEIPPEKVFLAQKQ
ITARCNRAGKPCICATQMLESMTNKPRATRAEISDVANAVLDGADCTMLSGETAKGEYPL
ECITTMAKTQMEAEAAIWHKNLFRDLVSLQPTPIDATHSIAISAVEAASKTLASCIICLT
TSGRSAHLISKYRPRCPIITVTRFEQTARQCHLFRGILPLIYQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g160.t2 Gene3D G3DSA:3.40.1380.20 - 16 463 6.5E-212
18 g160.t2 Gene3D G3DSA:3.20.20.60 - 33 373 6.5E-212
17 g160.t2 Gene3D G3DSA:2.40.33.10 - 107 207 6.5E-212
3 g160.t2 PANTHER PTHR11817:SF100 PYRUVATE KINASE 10 462 1.1E-205
4 g160.t2 PANTHER PTHR11817 PYRUVATE KINASE 10 462 1.1E-205
7 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 96 112 1.7E-76
9 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 225 239 1.7E-76
11 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 255 281 1.7E-76
6 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 282 306 1.7E-76
10 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 307 331 1.7E-76
5 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 332 350 1.7E-76
8 g160.t2 PRINTS PR01050 Pyruvate kinase family signature 351 367 1.7E-76
2 g160.t2 Pfam PF00224 Pyruvate kinase, barrel domain 33 382 1.6E-154
1 g160.t2 Pfam PF02887 Pyruvate kinase, alpha/beta domain 399 462 4.5E-21
15 g160.t2 ProSitePatterns PS00110 Pyruvate kinase active site signature. 253 265 -
14 g160.t2 SUPERFAMILY SSF51621 Phosphoenolpyruvate/pyruvate domain 18 382 4.89E-101
12 g160.t2 SUPERFAMILY SSF50800 PK beta-barrel domain-like 107 206 1.36E-26
13 g160.t2 SUPERFAMILY SSF52935 PK C-terminal domain-like 361 462 4.97E-25
19 g160.t2 TIGRFAM TIGR01064 pyruv_kin: pyruvate kinase 34 462 3.5E-171

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0030955 potassium ion binding MF
GO:0000287 magnesium ion binding MF
GO:0004743 pyruvate kinase activity MF
GO:0006096 glycolytic process BP
GO:0003824 catalytic activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values