General information for g16070.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as MAP/microtubule affinity-regulating kinase 3.
Parent gene
- The parent gene g16070 is located on chr_4: 7977618..7989245 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16070 | g16070.t1 | isoform | g16070.t1 | 7974694 | 7989245 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon1 | 7974694 | 7974881 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS1 | 7974694 | 7974881 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon2 | 7974930 | 7974959 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS2 | 7974930 | 7974959 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon3 | 7975019 | 7975137 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS3 | 7975019 | 7975137 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon4 | 7975198 | 7975347 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS4 | 7975198 | 7975347 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon5 | 7976370 | 7976411 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS5 | 7976370 | 7976411 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon6 | 7979103 | 7979199 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS6 | 7979103 | 7979199 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon7 | 7979272 | 7979369 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS7 | 7979272 | 7979369 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon8 | 7981601 | 7981778 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS8 | 7981601 | 7981778 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon9 | 7981976 | 7982097 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS9 | 7981976 | 7982097 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon10 | 7982160 | 7982259 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS10 | 7982160 | 7982259 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon11 | 7982378 | 7982558 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS11 | 7982378 | 7982558 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon12 | 7982688 | 7982800 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS12 | 7982688 | 7982800 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon13 | 7985988 | 7986075 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS13 | 7985988 | 7986075 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon14 | 7986137 | 7986339 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS14 | 7986137 | 7986339 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon15 | 7986407 | 7986468 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS15 | 7986407 | 7986468 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon16 | 7986535 | 7986648 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS16 | 7986535 | 7986648 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon17 | 7986962 | 7987182 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS17 | 7986962 | 7987182 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon18 | 7987243 | 7987509 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS18 | 7987243 | 7987509 |
| chr_4 | g16070 | g16070.t1 | exon | g16070.t1.exon19 | 7988574 | 7989245 |
| chr_4 | g16070 | g16070.t1 | cds | g16070.t1.CDS19 | 7988574 | 7989245 |
| chr_4 | g16070 | g16070.t1 | TSS | g16070.t1 | 7989331 | 7989331 |
| chr_4 | g16070 | g16070.t1 | TTS | g16070.t1 | NA | NA |
Sequences
>g16070.t1 Gene=g16070 Length=3045
ATGAGTGAATTTGACAGAACAGAAAATATTAGACGATCAATTCGTCGTCCTAAAAAATCA
CAAGCACCACAACCGCCTATCGATACACAAAGACCATCCCCAACAGAGGATGTCTCATCA
ATCATTAAAAGACTTCTCAACACTCGACGTGATTTTGTTGCTACATCATCAACGACGCCA
GTCCCGTCAGTCATTCAACATCAGCAACAACCGCGAATTGTTTCAACAACACCAAAATCA
TCTATAGCTGATGATGACTCATCACACAATGCGTTATTGGCAATTCAACGTCACATCAAT
CAAATTGAATTTAATCAAAAAATTAACGAACATGAACACCAGCAGCATCAGCAGCAAACA
CAAGTGCCAATAAATGCATTAAGAAAAACACGCTATGATGAAATGGAATCGCTACTAAAT
ACAGGCATTAGTGATTTAGCTACAAATAAGCATGATACAGACGATGAAAGTGATGCAATT
GAATTTCAGCGTGGTGGTGGCACACGCAGTTCAATTCAGGGCGTGCCTGGTGCAGCTAAT
GACATTAAAAAATTAAGAATGCCTCGACCAGTTGCTCGTACTGCTAGTGACACAAAACAG
CATGAACTGCTGCGTGGACTTTTTAAACCTACAACGAATAAAAAGGAAAGTGTTGATGAT
CATGCGAACAAGGGTTCACCAAATATGCAAATGCGTGTTAGTAACAGTCATAATAGATGG
AGACCAGGTGAAGAGCAACAAAGTAGTGGAGCAAATCATACAAGATGGCGTCCAGCTGAA
GAACAAATTGGCAAATATCGACTGCTCAAGACAATCGGCAAGGGTAATTTTGCCAAAGTG
AAGCTCGCCAAGCATGTGCCAACAGGCAAAGAAGTTGCAATCAAAATCATTGACAAGACA
CAATTAAATCCCACCAGCTTGCAAAAGCTCTTTCGTGAAGTTCGTATCATGAAAATGCTT
GATCATCCAAACATTGTAAAATTATTTCAAGTAATTCAAACTGATAAAACACTCTACCTA
GTAATGGAATATGCATCAGGTGGTGAAGTTTTTGATTATCTTGTCATGCATGGTCGCATG
AAAGAAAAAGAAGCGCGTGCTAAATTCCGACAAATTGTCTCAGCCGTTCAGTATTTGCAT
CAAAAGCGCATCATTCATCGTGATTTAAAGGCTGAAAATCTGTTGCTTGACTCAGAAATG
AATATAAAAATTGCCGATTTTGGCTTTAGTAATGAATTTACTCCTGGCAATAAGCTAGAT
ACATTCTGTGGAAGTCCTCCTTATGCTGCACCAGAATTGTTTCAAGGCAGAAAATATGAT
GGACCTGAAGTTGATGTATGGTCACTTGGTGTAATTCTTTATACACTAGTAAGTGGCTCA
CTTCCGTTTGATGGTACAACGTTGCGTGAATTACGTGAACGAGTGTTACGTGGCAAGTAT
CGTATACCATTCTACATATCCACCGACTGCGAAAATTTACTGAGGAAATTCCTCGTGCTG
AATCCAGCAAAGCGTGCAAGCTTAGAACAGATTATGAAGGATAAATGGATGAATATGGGC
TATGAAGATGATGAGCTAAAACCTTATGTTGAACCACAGCCTGATCTCAATGATCAGAAA
AGGATAGAATGCTTAGTGGCGCTGGGCTATAAGCGAGAAGACATTGAGGCATCACTGTCA
CAAACACGATACGATGATGTGCATGCAACTTATTTACTAATTAGTCGTAAAAACACAGAT
CCGGAGTCAGATGGTTCCAGATCAGGTTCATCTCTTTCATTACGTAATTTAACAGGCGAT
GCAGCACAGCAAGGTGCAAATAGTCAAGTGCAATCGCCAACACATCGTCGCGTTCATCGC
AGTATTTCCGCTACAAGCACTAATCCATCGCGACGTGCATCATCAGGTGGTGAAACTTTG
AGAGCAGCAAATGCAGTTCCAACAACAAATGCTACCAATGGTACCACATCAACAACAGCA
TCAAATGCATCATCGTCAGCAGCGCCAGAACGATCAACAATTAGCAGTAACTTTAAAAGA
CAAAACACAATCGATGCAGCAACAATTAAAGAAAATACAGCTCGATTAGCGGCACAAAAT
CAAAGGCCAGTATCAGCACAACCCAAATCAGCAACAGAATCATCGACTTCAGCAACATCA
CCAGTGAAGCCTCGATCAACAGCCACAAAATATGATGCAGCATCAAGTGGTAATAGAACA
GTTGGACCAGCTTCTGGTGGTGGACTCATTCCACGTCGTTCAACGACATTATATGAAAAG
ACATCATCTACTGAAAAAACTAATGCGTCCACTGATAATACTTTGACGAATCGCATCAGT
TTGACACCCGCCAATACTTCCAGGCAACAATCGGCTGCATTTCCCAGAAATGTGCCGTCA
CGATCAACTTTTCATTCTGGACAAACAAGAACACGAAATAGTTCATCAGGTGCAGCAACT
GGTGATGCAAGCAAAGGAAGCTTCTTGCAAAGAATTTCAAATCGTTTCAGCAAACGACCG
ATCGATGGCCAAGCTAACATTACAAGCACACCAAATAGCGGTTCCAATGCTGAAGAGCAG
GTAAAGCCACGTGCCCTAAGATTTACATGGTCTATGAAAACAACCTCACCAAGACTGCCT
GATGATATCATGGCTGAGATTAGATCTGTTTTAGATAAAAATAATTGCGATTATGAACAA
AGAGAAAGATTTGTTCTTTTGTGTGTCCATGGTGATCCAAATACTGATTCATTAGTACAG
TGGGAGATTGAAGTCTGCAAACTGCCTCGTCTGTCATTAAATGGTGTTCGATTTAAGAGA
ATTTCAGGCACAAGCATTGGATTCAAGAATATTGCTACTACCACCGCATCAACGACAGAT
AAAAACGTCACTACAAATAATAACAACAACATTCCAACGACAATTCATGACAAATTAGTC
GATGAAAGTGATGAATGGAGTTTTGATAGTGGCGAAGATGTTATCGAGATCTCACTTAAT
GAATCTGCAAGTGCAAAAAGCGGTGGAGGTGGTGGCAAGAAGTGA
>g16070.t1 Gene=g16070 Length=1014
MSEFDRTENIRRSIRRPKKSQAPQPPIDTQRPSPTEDVSSIIKRLLNTRRDFVATSSTTP
VPSVIQHQQQPRIVSTTPKSSIADDDSSHNALLAIQRHINQIEFNQKINEHEHQQHQQQT
QVPINALRKTRYDEMESLLNTGISDLATNKHDTDDESDAIEFQRGGGTRSSIQGVPGAAN
DIKKLRMPRPVARTASDTKQHELLRGLFKPTTNKKESVDDHANKGSPNMQMRVSNSHNRW
RPGEEQQSSGANHTRWRPAEEQIGKYRLLKTIGKGNFAKVKLAKHVPTGKEVAIKIIDKT
QLNPTSLQKLFREVRIMKMLDHPNIVKLFQVIQTDKTLYLVMEYASGGEVFDYLVMHGRM
KEKEARAKFRQIVSAVQYLHQKRIIHRDLKAENLLLDSEMNIKIADFGFSNEFTPGNKLD
TFCGSPPYAAPELFQGRKYDGPEVDVWSLGVILYTLVSGSLPFDGTTLRELRERVLRGKY
RIPFYISTDCENLLRKFLVLNPAKRASLEQIMKDKWMNMGYEDDELKPYVEPQPDLNDQK
RIECLVALGYKREDIEASLSQTRYDDVHATYLLISRKNTDPESDGSRSGSSLSLRNLTGD
AAQQGANSQVQSPTHRRVHRSISATSTNPSRRASSGGETLRAANAVPTTNATNGTTSTTA
SNASSSAAPERSTISSNFKRQNTIDAATIKENTARLAAQNQRPVSAQPKSATESSTSATS
PVKPRSTATKYDAASSGNRTVGPASGGGLIPRRSTTLYEKTSSTEKTNASTDNTLTNRIS
LTPANTSRQQSAAFPRNVPSRSTFHSGQTRTRNSSSGAATGDASKGSFLQRISNRFSKRP
IDGQANITSTPNSGSNAEEQVKPRALRFTWSMKTTSPRLPDDIMAEIRSVLDKNNCDYEQ
RERFVLLCVHGDPNTDSLVQWEIEVCKLPRLSLNGVRFKRISGTSIGFKNIATTTASTTD
KNVTTNNNNNIPTTIHDKLVDESDEWSFDSGEDVIEISLNESASAKSGGGGGKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g16070.t1 | CDD | cd14072 | STKc_MARK | 265 | 517 | 0.0 |
| 13 | g16070.t1 | CDD | cd14337 | UBA_MARK_Par1 | 537 | 576 | 3.45198E-13 |
| 12 | g16070.t1 | CDD | cd12196 | MARK1-3_C | 862 | 956 | 9.17457E-60 |
| 8 | g16070.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 252 | 346 | 5.0E-37 |
| 9 | g16070.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 347 | 532 | 4.3E-67 |
| 7 | g16070.t1 | Gene3D | G3DSA:1.10.8.10 | DNA helicase RuvA subunit | 533 | 577 | 4.6E-21 |
| 10 | g16070.t1 | Gene3D | G3DSA:3.30.310.80 | Kinase associated domain 1 | 850 | 962 | 2.5E-43 |
| 27 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 15 | - |
| 29 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 38 | - |
| 26 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 23 | 38 | - |
| 24 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 56 | 83 | - |
| 25 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 209 | 257 | - |
| 19 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 223 | 255 | - |
| 22 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 601 | 680 | - |
| 21 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 695 | 742 | - |
| 28 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 695 | 827 | - |
| 18 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 755 | 827 | - |
| 20 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 839 | 861 | - |
| 23 | g16070.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 839 | 859 | - |
| 3 | g16070.t1 | PANTHER | PTHR24346 | MAP/MICROTUBULE AFFINITY-REGULATING KINASE | 244 | 848 | 8.8E-240 |
| 4 | g16070.t1 | PANTHER | PTHR24346:SF56 | SERINE/THREONINE-PROTEIN KINASE MARK2 | 244 | 848 | 8.8E-240 |
| 2 | g16070.t1 | Pfam | PF00069 | Protein kinase domain | 266 | 517 | 8.8E-76 |
| 1 | g16070.t1 | Pfam | PF02149 | Kinase associated domain 1 | 919 | 953 | 5.8E-15 |
| 15 | g16070.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 272 | 295 | - |
| 14 | g16070.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 384 | 396 | - |
| 32 | g16070.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 266 | 517 | 54.463 |
| 30 | g16070.t1 | ProSiteProfiles | PS50030 | Ubiquitin-associated domain (UBA) profile. | 535 | 575 | 9.511 |
| 31 | g16070.t1 | ProSiteProfiles | PS50032 | Kinase associated domain 1 (KA1) profile. | 912 | 961 | 15.07 |
| 17 | g16070.t1 | SMART | SM00220 | serkin_6 | 266 | 517 | 5.2E-110 |
| 16 | g16070.t1 | SMART | SM00165 | uba_6 | 538 | 575 | 2.5E-5 |
| 6 | g16070.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 263 | 518 | 2.83E-97 |
| 5 | g16070.t1 | SUPERFAMILY | SSF103243 | KA1-like | 855 | 957 | 9.16E-40 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08798 (MAP/microtubule affinity-regulating kinase)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
