General information for g16096.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Cathepsin O.
Parent gene
- The parent gene g16096 is located on chr_4: 8140956..8142166 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16096 | g16096.t1 | isoform | g16096.t1 | 8140956 | 8142166 |
| chr_4 | g16096 | g16096.t1 | exon | g16096.t1.exon1 | 8140956 | 8141085 |
| chr_4 | g16096 | g16096.t1 | cds | g16096.t1.CDS1 | 8140956 | 8141085 |
| chr_4 | g16096 | g16096.t1 | exon | g16096.t1.exon2 | 8141137 | 8141310 |
| chr_4 | g16096 | g16096.t1 | cds | g16096.t1.CDS2 | 8141137 | 8141310 |
| chr_4 | g16096 | g16096.t1 | exon | g16096.t1.exon3 | 8141389 | 8141700 |
| chr_4 | g16096 | g16096.t1 | cds | g16096.t1.CDS3 | 8141389 | 8141700 |
| chr_4 | g16096 | g16096.t1 | exon | g16096.t1.exon4 | 8141751 | 8142166 |
| chr_4 | g16096 | g16096.t1 | cds | g16096.t1.CDS4 | 8141751 | 8142166 |
| chr_4 | g16096 | g16096.t1 | TSS | g16096.t1 | NA | NA |
| chr_4 | g16096 | g16096.t1 | TTS | g16096.t1 | NA | NA |
Sequences
>g16096.t1 Gene=g16096 Length=1032
ATGCGTGAAGAAATTACAGTTTTAACAATGATGTTTTTGTTTTGTGCTGCAACAATTCCC
ATCATTGTTATCTCAAGTGCAAGCAAAAATTTTGTAATAAGAAATTACAGTGTTAGCGAT
CAAAAGCTTTCATATCAATTTTATCAAATGAATTTTCGTGACAGGTCAGCAATGAATATC
GTAAGAATTATTGAAAGTCGGAAAAATTCAAGCAAATATCAAGGAGTTGATCTGAAAATC
TTTCAAAATATTGACTCTGATCATTTTTTTGAAAAGTATCAAAAAATTATACCAAATCAA
AAATATTTAAGTTATTTTTCAAAAGAAAATATTGAAAAGATTTGGATGAAATTTAATTTT
TACAATAAAACATTGTTAAAATCTAATGGAATACTCATACCTTTAAAAGTTGATTGGCGA
AAAATTGGAATAATCTCAGAAGTTAAAAAACAAACAAAATGCGGTGATTGTTGGAATATA
GCAACAATTGGTCTGATTGAAAGCATGTTGGCATTAAAAACTGGCAAATTAGAAGAACTT
AGTACAACCCAAATTCTTCAATGCAATGATGAAGGTATGGATTGCAAAGGTGGTGATTTT
TATCGACTATTAAGATGGCTTTATGAAGACAAAATTTATATTAAAACTGAGAAAGAATTC
ATTGAATCAAATTACAATTGTAAGAGAAGAAAACCTGGAATACGCGTTAAAGATTTTTCA
TTTAATAATTTTACAGGAAATGAACATTTAATGCTGCTACATTTAGCACGTCAAGGACCT
TTAGTTACTGCGGTAAACTCTTTAATGTGGAAAGAATACACAAACAAAGTTATTGAGAAA
AATTGCAATGGAAATTCAAAATTGATTAATCATGCTGTAGAAATTGTTGGCTATGATATG
ACGGCAAACACTCCATATTATATCGTTAAAAACTCATGGGGAAGTCAATGGGGAATAAAT
GGTTATATTCACATTGCAATCGGTGATAATTTATGTGGAATTGCTGAAAGAGTTTCAAGT
GTAATTGTTTAA
>g16096.t1 Gene=g16096 Length=343
MREEITVLTMMFLFCAATIPIIVISSASKNFVIRNYSVSDQKLSYQFYQMNFRDRSAMNI
VRIIESRKNSSKYQGVDLKIFQNIDSDHFFEKYQKIIPNQKYLSYFSKENIEKIWMKFNF
YNKTLLKSNGILIPLKVDWRKIGIISEVKKQTKCGDCWNIATIGLIESMLALKTGKLEEL
STTQILQCNDEGMDCKGGDFYRLLRWLYEDKIYIKTEKEFIESNYNCKRRKPGIRVKDFS
FNNFTGNEHLMLLHLARQGPLVTAVNSLMWKEYTNKVIEKNCNGNSKLINHAVEIVGYDM
TANTPYYIVKNSWGSQWGINGYIHIAIGDNLCGIAERVSSVIV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g16096.t1 | CDD | cd02248 | Peptidase_C1A | 134 | 341 | 5.05096E-62 |
| 5 | g16096.t1 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 27 | 343 | 2.0E-56 |
| 2 | g16096.t1 | PANTHER | PTHR12411 | CYSTEINE PROTEASE FAMILY C1-RELATED | 52 | 335 | 1.7E-40 |
| 3 | g16096.t1 | PANTHER | PTHR12411:SF775 | CATHEPSIN O-RELATED | 52 | 335 | 1.7E-40 |
| 1 | g16096.t1 | Pfam | PF00112 | Papain family cysteine protease | 133 | 341 | 4.3E-48 |
| 7 | g16096.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 27 | - |
| 8 | g16096.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 11 | - |
| 9 | g16096.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 12 | 23 | - |
| 10 | g16096.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 24 | 27 | - |
| 6 | g16096.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 28 | 343 | - |
| 13 | g16096.t1 | ProSitePatterns | PS00639 | Eukaryotic thiol (cysteine) proteases histidine active site. | 289 | 299 | - |
| 14 | g16096.t1 | SMART | SM00645 | pept_c1 | 133 | 342 | 1.3E-37 |
| 4 | g16096.t1 | SUPERFAMILY | SSF54001 | Cysteine proteinases | 41 | 342 | 7.02E-57 |
| 12 | g16096.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 27 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008234 | cysteine-type peptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
- This transcript has been annotated with K01374 (cathepsin O)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed