Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Glutathione S-transferase theta-1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16120 g16120.t1 TSS g16120.t1 8240936 8240936
chr_4 g16120 g16120.t1 isoform g16120.t1 8240977 8241714
chr_4 g16120 g16120.t1 exon g16120.t1.exon1 8240977 8241188
chr_4 g16120 g16120.t1 cds g16120.t1.CDS1 8240977 8241188
chr_4 g16120 g16120.t1 exon g16120.t1.exon2 8241243 8241714
chr_4 g16120 g16120.t1 cds g16120.t1.CDS2 8241243 8241714
chr_4 g16120 g16120.t1 TTS g16120.t1 8241932 8241932

Sequences

>g16120.t1 Gene=g16120 Length=684
ATGAAATATTATTATAATTTACTTTCACAACCATCACGAGCTCTTTACATTTTTCTTAAG
CTCAATAACATTCCAGTTGAGTACAAAGATGTTAATTTATTAAATGGTGATCATTTAACA
GATGAGTTTAGAGAAATTAATCGTTTCCAAAAAGTTCCTTGTATTGTTGATGATGATGGA
TGGAAATTGGCAGAAAGTATTGCAATTTTAAGATATTTAATGTCAAAATACAAAAATATT
CCCGACCATTTTTATCCATCTGAAATAAGAGCAAGAGCAGCTGTTGATGAATATCTTGAA
TGGCAACATTTAAACACTCGTTTAGGTTGTGCCATTTATTTTCAAATTAAAAAGAAAATT
GGTCCATTTGCAGCAATATCAGAAAATCCTAACGAAGGAGCACTTCCAATTTTTAAATTT
CTTATGGAATCAACTTTAGAAGCAATTGAAACTTTGTGGTTGAGAGGAAAGAAATTTTTA
TGTTCTGATGAAATTTCTTTTGCTGATATAATTGCTGCTTGTGAAATTGAACAACCTCGT
ATGACGGGTTATGACATTTTTGAAAATCGTCCAAAATTAAAGGAATGGTACGAAAGAGTT
AGGGCTGCAACAAATCCACATTATGATGAAGCTCATGTGATTGTTAATAAAATTATAGCA
AGAAGTCAAAAAGCTAAACTTTAA

>g16120.t1 Gene=g16120 Length=227
MKYYYNLLSQPSRALYIFLKLNNIPVEYKDVNLLNGDHLTDEFREINRFQKVPCIVDDDG
WKLAESIAILRYLMSKYKNIPDHFYPSEIRARAAVDEYLEWQHLNTRLGCAIYFQIKKKI
GPFAAISENPNEGALPIFKFLMESTLEAIETLWLRGKKFLCSDEISFADIIAACEIEQPR
MTGYDIFENRPKLKEWYERVRAATNPHYDEAHVIVNKIIARSQKAKL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g16120.t1 CDD cd03050 GST_N_Theta 2 77 0.000
11 g16120.t1 CDD cd03183 GST_C_Theta 91 218 0.000
7 g16120.t1 Gene3D G3DSA:3.40.30.10 Glutaredoxin 1 79 0.000
6 g16120.t1 Gene3D G3DSA:1.20.1050.10 - 81 226 0.000
3 g16120.t1 PANTHER PTHR43917 - 2 225 0.000
2 g16120.t1 Pfam PF13417 Glutathione S-transferase, N-terminal domain 9 78 0.000
1 g16120.t1 Pfam PF00043 Glutathione S-transferase, C-terminal domain 153 201 0.000
9 g16120.t1 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 1 81 21.020
8 g16120.t1 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 88 227 12.587
12 g16120.t1 SFLD SFLDG01153 Main.4: Theta-like 1 224 0.000
13 g16120.t1 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 1 224 0.000
4 g16120.t1 SUPERFAMILY SSF52833 Thioredoxin-like 1 92 0.000
5 g16120.t1 SUPERFAMILY SSF47616 GST C-terminal domain-like 82 203 0.000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values