General information for g16136.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cofilin/actin-depolymerizing factor-like protein.
Parent gene
- The parent gene g16136 is located on chr_4: 8338443..8342935 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16136 | g16136.t20 | TTS | g16136.t20 | 8338183 | 8338183 |
| chr_4 | g16136 | g16136.t20 | isoform | g16136.t20 | 8338443 | 8342707 |
| chr_4 | g16136 | g16136.t20 | exon | g16136.t20.exon1 | 8338443 | 8338589 |
| chr_4 | g16136 | g16136.t20 | cds | g16136.t20.CDS1 | 8338443 | 8338589 |
| chr_4 | g16136 | g16136.t20 | exon | g16136.t20.exon2 | 8338651 | 8338893 |
| chr_4 | g16136 | g16136.t20 | cds | g16136.t20.CDS2 | 8338651 | 8338893 |
| chr_4 | g16136 | g16136.t20 | exon | g16136.t20.exon3 | 8342705 | 8342707 |
| chr_4 | g16136 | g16136.t20 | cds | g16136.t20.CDS3 | 8342705 | 8342707 |
| chr_4 | g16136 | g16136.t20 | TSS | g16136.t20 | 8342954 | 8342954 |
Sequences
>g16136.t20 Gene=g16136 Length=393
ATGAAGGACAAAAAACATCGCTATGTAATTTTTTACATTCGTGATGAGAAGCAAATTGAT
GTGGAAGTGATTGGTGAACGTAATGCAGAATATGATTCGTTCCTTGAAGATATTCAAAAA
GGCGGTCCAGGTGAATGCCGATATGGACTCTTTGATTTTGAGTATATGCATCAATGTCAA
GGCACCTCTGAGAGTTCCAAGAAGCAAAAATTATTTTTAATGTCCTGGTGTCCAGATACT
GCTAAAGTCAAGAAGAAGATGTTGTACTCAAGCTCATTTGATGCATTGAAAAAGTCACTT
GTAGGTGTGCAAAAATACATTCAAGCTACAGACTTATCAGAGGCCTCACGTGAAGCTGTT
GAAGAAAAATTGCGCTCTACTGATCGCAACTAA
>g16136.t20 Gene=g16136 Length=130
MKDKKHRYVIFYIRDEKQIDVEVIGERNAEYDSFLEDIQKGGPGECRYGLFDFEYMHQCQ
GTSESSKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASREAV
EEKLRSTDRN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g16136.t20 | CDD | cd11286 | ADF_cofilin_like | 1 | 124 | 0.000 |
| 6 | g16136.t20 | Gene3D | G3DSA:3.40.20.10 | Severin | 1 | 130 | 0.000 |
| 2 | g16136.t20 | PANTHER | PTHR11913:SF12 | COFILIN/ACTIN-DEPOLYMERIZING FACTOR HOMOLOG-RELATED | 3 | 125 | 0.000 |
| 3 | g16136.t20 | PANTHER | PTHR11913 | COFILIN-RELATED | 3 | 125 | 0.000 |
| 1 | g16136.t20 | Pfam | PF00241 | Cofilin/tropomyosin-type actin-binding protein | 3 | 123 | 0.000 |
| 7 | g16136.t20 | ProSiteProfiles | PS51263 | ADF-H domain profile. | 1 | 125 | 32.286 |
| 5 | g16136.t20 | SMART | SM00102 | adf_2 | 2 | 125 | 0.000 |
| 4 | g16136.t20 | SUPERFAMILY | SSF55753 | Actin depolymerizing proteins | 3 | 126 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003779 | actin binding | MF |
| GO:0015629 | actin cytoskeleton | CC |
| GO:0030042 | actin filament depolymerization | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
