General information for g16221.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Potassium channel subfamily K member 10.
Parent gene
- The parent gene g16221 is located on chr_4: 8641829..8646481 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16221 | g16221.t1 | TSS | g16221.t1 | 8641662 | 8641662 |
| chr_4 | g16221 | g16221.t1 | isoform | g16221.t1 | 8641829 | 8646481 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon1 | 8641829 | 8642071 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS1 | 8641829 | 8642071 |
| chr_4 | g16221 | g16221.t1 | TTS | g16221.t1 | 8642393 | 8642393 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon2 | 8645046 | 8645361 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS2 | 8645046 | 8645361 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon3 | 8645431 | 8645586 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS3 | 8645431 | 8645586 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon4 | 8645652 | 8645837 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS4 | 8645652 | 8645837 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon5 | 8645897 | 8645937 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS5 | 8645897 | 8645937 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon6 | 8646083 | 8646265 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS6 | 8646083 | 8646265 |
| chr_4 | g16221 | g16221.t1 | exon | g16221.t1.exon7 | 8646326 | 8646481 |
| chr_4 | g16221 | g16221.t1 | cds | g16221.t1.CDS7 | 8646326 | 8646481 |
Sequences
>g16221.t1 Gene=g16221 Length=1281
ATGGGCGATGATATTTTTATAAAACGTGTAGATTTTGAAGTAAATAATAACGAACCAAAT
ACAGTAATTCCAAATGGTCATGAAATGTTGACAGATGATGTAGAAAATTTGAATAAACCT
GGACCACCACCAACATGTTGTGGCAAATTTTTATATTATCTTGATAAATTTGGGATCAAA
GCAGCACTAAGTCATGTTGGGTTGCTTTTAGGATTAGCTCTTTATTGTTATGCAGGCGGA
TGGGTGTTTGTCCAACTAGAACATCCTCCAATGAAAGAAAATGAATATGAACTAAGAAAT
AACATTTCGATCAGCCGTTCTGAATTTATTCAAATAATTATTGATGCACACAATGATAAT
GATTTAAATGAGAATTTTATATCAGAAAAATTACAAGAATATGAAATGGTAGCACAAGCG
GCAGTTGAAGGAAATTTGCAGCTTACAAATACAGCTGGGAATATTACAAATACAAATGAT
ATTGCATTTCCAGTGCAATCTGAAAAATGGACATTAATGCAAGCAATCTTTTTTGCATCA
ACAATTTGCACAACAATTGGTTATGGAAATATTGCACCAGAAACTTTCGAAGGACGATTA
TTTTGCATCATCTTTGCTATTTTTGGGCTCCCATTTACGCTCACTGTAATTGCTGATTAT
GGTAGCTTATTTGCTAATTCAGTATCAGCTGTAGCTAAAAAATGTAAAACATTCAAATTT
TGCTCAAAAGATTCAAAATTTAGAACATTCAAAGGTCGAAAGTGGTTATATGCTATTGGA
GCTGTAATTTTCTTAGGATTTTATTTATCAGCTGGTGCAGCAGTTTTTGAACAATGGGAG
CATTGGACATTTTTTGAAGGCTTTTATTTTAGTTTTATCACTATGACAACTATTGGTTTA
GGTGATCTTGTTCCAGGTGAAAAACCAATGTTAATTTGCACCGTCTACATTCTTATTGGA
TTAGCATTAACAAGTACAATTCTTGAACTCGTAAGACGGCAATATGTTCAAAGCTGGCAA
AAATTACAACAATTAAAACTTGGTTCTTTTGCTGACTCATTGCGACGTCTAGGTGAATCT
CATGGTGCTATTGATGTCAATGATTTACGTTCAATTCTCTCAGTTGTTTCAATGTCAAAG
AAAGGAAACAAAAAAGGTTCAGATAATTCCGACTCAGATCCAATTGAAGCATTGACTAAA
GAAATTTTACAAAAAGTTAAAATTAAACAGATGGAAAAACCACAATTGGTTCAAATTATT
ATTTATGAATCTTCAGTATAA
>g16221.t1 Gene=g16221 Length=426
MGDDIFIKRVDFEVNNNEPNTVIPNGHEMLTDDVENLNKPGPPPTCCGKFLYYLDKFGIK
AALSHVGLLLGLALYCYAGGWVFVQLEHPPMKENEYELRNNISISRSEFIQIIIDAHNDN
DLNENFISEKLQEYEMVAQAAVEGNLQLTNTAGNITNTNDIAFPVQSEKWTLMQAIFFAS
TICTTIGYGNIAPETFEGRLFCIIFAIFGLPFTLTVIADYGSLFANSVSAVAKKCKTFKF
CSKDSKFRTFKGRKWLYAIGAVIFLGFYLSAGAAVFEQWEHWTFFEGFYFSFITMTTIGL
GDLVPGEKPMLICTVYILIGLALTSTILELVRRQYVQSWQKLQQLKLGSFADSLRRLGES
HGAIDVNDLRSILSVVSMSKKGNKKGSDNSDSDPIEALTKEILQKVKIKQMEKPQLVQII
IYESSV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g16221.t1 | Gene3D | G3DSA:1.10.287.70 | - | 61 | 344 | 5.8E-68 |
| 3 | g16221.t1 | PANTHER | PTHR11003 | POTASSIUM CHANNEL, SUBFAMILY K | 113 | 382 | 1.6E-77 |
| 4 | g16221.t1 | PANTHER | PTHR11003:SF142 | TWIK FAMILY OF POTASSIUM CHANNELS PROTEIN 7-LIKE PROTEIN | 113 | 382 | 1.6E-77 |
| 5 | g16221.t1 | PRINTS | PR01333 | Two pore domain K+ channel signature | 181 | 209 | 4.8E-16 |
| 6 | g16221.t1 | PRINTS | PR01333 | Two pore domain K+ channel signature | 296 | 305 | 4.8E-16 |
| 1 | g16221.t1 | Pfam | PF07885 | Ion channel | 167 | 224 | 1.3E-15 |
| 2 | g16221.t1 | Pfam | PF07885 | Ion channel | 264 | 333 | 2.5E-19 |
| 17 | g16221.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 61 | - |
| 27 | g16221.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 62 | 84 | - |
| 21 | g16221.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 85 | 169 | - |
| 28 | g16221.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 170 | 188 | - |
| 18 | g16221.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 189 | 199 | - |
| 24 | g16221.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 200 | 218 | - |
| 22 | g16221.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 219 | 254 | - |
| 26 | g16221.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 255 | 276 | - |
| 19 | g16221.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 277 | 282 | - |
| 25 | g16221.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 283 | 303 | - |
| 20 | g16221.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 304 | 308 | - |
| 23 | g16221.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 309 | 331 | - |
| 16 | g16221.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 332 | 426 | - |
| 14 | g16221.t1 | SUPERFAMILY | SSF81324 | Voltage-gated potassium channels | 62 | 231 | 4.84E-21 |
| 13 | g16221.t1 | SUPERFAMILY | SSF81324 | Voltage-gated potassium channels | 247 | 345 | 7.98E-21 |
| 11 | g16221.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 62 | 84 | - |
| 12 | g16221.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 170 | 192 | - |
| 8 | g16221.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 199 | 218 | - |
| 10 | g16221.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 255 | 277 | - |
| 9 | g16221.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 282 | 304 | - |
| 7 | g16221.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 309 | 331 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005267 | potassium channel activity | MF |
| GO:0071805 | potassium ion transmembrane transport | BP |
KEGG
Orthology
- This transcript has been annotated with K05323 (potassium channel subfamily K, invertebrate)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed