General information for g16349.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as histone-arginine N-methyltransferase.
Parent gene
- The parent gene g16349 is located on chr_4: 9162753..9169984 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16349 | g16349.t1 | TTS | g16349.t1 | 9160697 | 9160697 |
| chr_4 | g16349 | g16349.t1 | isoform | g16349.t1 | 9160758 | 9162876 |
| chr_4 | g16349 | g16349.t1 | exon | g16349.t1.exon1 | 9160758 | 9161081 |
| chr_4 | g16349 | g16349.t1 | cds | g16349.t1.CDS1 | 9160758 | 9161081 |
| chr_4 | g16349 | g16349.t1 | exon | g16349.t1.exon2 | 9161177 | 9162686 |
| chr_4 | g16349 | g16349.t1 | cds | g16349.t1.CDS2 | 9161177 | 9162686 |
| chr_4 | g16349 | g16349.t1 | exon | g16349.t1.exon3 | 9162746 | 9162876 |
| chr_4 | g16349 | g16349.t1 | cds | g16349.t1.CDS3 | 9162746 | 9162876 |
| chr_4 | g16349 | g16349.t1 | TSS | g16349.t1 | 9162941 | 9162941 |
Sequences
>g16349.t1 Gene=g16349 Length=1965
ATGAGCACACCAAAAACTCCAAAAGTAAGTCTTGAATTTAAGAAAAAATTACGACAAGAA
TATGAAAGATTAAAGGCACTTCAAGAATCTCAAGAAAAGGAAATAATTGCAGTAACTTGG
GAAAATAATCGAAAGCAATGCAAGGCCAATGTTGATGAGATCAATGAACGCTTGGCAAAT
ATCAATTGTGTTTTTCAAGATGATGCCTACAACAACATTGAGCACAAAGATCATTTAAAA
AAGGCTTCAATTACAAGTCATGATGGTGTAAAGCAATCAGTACCAACATATGTCATGTCT
AATGTCATTCCAATACCAAATATGTGTGCATGGGCACCAGTACAGCAAAATTTCATGGTC
GAAGATGAGACAGTACTCTATAACATCCCCTATATGGGTGATGAAGTTCTAGACAAAGAT
TCAGACTTTATTGATGAATTAATTAAAAATTATGATGGAAAAGTTCATGGTGATAATGAT
GCAGAATTTATTGATGATAAATTATTTGTAGATTTAGTTAAAGCAATGATTCCACATCAA
AGTTCTGAACCATCAACAAGTGAAGCTTTTCCAAGTCAAATGACTTTCCAAGCTGTCAGT
GAAATGTTCCCGAGTAAAGGCACACCTGAAGAACTTCGACAAAATTATATAGAACTCACA
AAAAGTGTTGATCCAACTCGACCACCAGAATTTTGCACACCAAACATAGATGGACCAAAT
GCTGAATCAGTCAGTCATGATCAAACATTGCATTCTTTTCATGCACTTTTTTGTCGTCGA
TGTTTCAAATATGATTGTTTTTTGCATCGACTTTCAACACATCCTGGACCAAATTTACAA
AAACGTAAAGGACCAGAAGTTGAATCAAGTGGTGAGCCATGTGGTCCAGATTGCTATTTA
CATATTGTCCCTAAACAAACTGAACCAAGTCCTAAGCAACAAGATCAACGTCAAAAAGCA
ATTGATAGATCAAAATTTACTCTTGAATCAAGTCCAAGTCCAATGGAAGCTGATGATGTC
TGGACTGGCTCAGATCAGTCATTTTTCCGTGTTCTTCATCGTTGTTTTTTGGACAATTAT
TGTGCAATTGCTAAAGGAATGCAAACAAAAACATGTCGACAAGTTTATGAATTTGCACAA
GAAGAACTTCAACATACTGAGCCATTAGAATTAGGAAAACATGCATCACCACCAAGAAAA
CAACAAAAGAAGAAAATTAATACAAAATTATGGACAATGCATATTCGTAAAAATGCCAGA
AAAGAAGCAAATGCTAACAATGTTTATAAATACACACCATGTGATCATAGTGGACCATGT
GGTGCAGGCTGTTGCTGCTTTGACAGTTCAAATTATTGTGAAAAATTCTGCAATTGTTCA
TCTGATTGTGCTAATCGCTTTCCTGGATGTCGATGCAAAGCTCAATGTAATACAAAACAA
TGCAGTTGTTATTTGGCTGTTAGGGAATGCGATCCTGATTTATGTCAATGTGGTGCACAT
CAATTCCATTTGAATAAAATGACTTGTAAGAATGTTAGTGTACAGAGAGGTCTAAGAAAG
CATTTATTGATGGCACCGAGCGATATTGCTGGTTGGGGAATTTTCTTAAAAGATGGAGCT
CAGAAAAATGAATTTATTAGTGAATACTGCGGTGAAATAATCAGTCAAGATGAAGCTGAT
CGTCGTGGAAAAGTTTATGATAAATATGCATGCAGTTTTCTTTTCAATCTTAATAATGAC
TTTGTCGTTGATGCCACAAGAAAAGGAAATAAAATTCGCTTTGCAAATCATTCAATAAAT
CCAAATTGTTATGCAAAAGTCATCATGGTTAATGGCGATCATAGAATTGGAATTTTTGCT
AAAAGAATTATTCAACCTGGTGAAGAATTGTTCTTTGATTATCGTTATGGACCAACTGAA
CAGTTGAAATTTGTTGGAATTGAAAGAGAAATGGAAATTTTATAA
>g16349.t1 Gene=g16349 Length=654
MSTPKTPKVSLEFKKKLRQEYERLKALQESQEKEIIAVTWENNRKQCKANVDEINERLAN
INCVFQDDAYNNIEHKDHLKKASITSHDGVKQSVPTYVMSNVIPIPNMCAWAPVQQNFMV
EDETVLYNIPYMGDEVLDKDSDFIDELIKNYDGKVHGDNDAEFIDDKLFVDLVKAMIPHQ
SSEPSTSEAFPSQMTFQAVSEMFPSKGTPEELRQNYIELTKSVDPTRPPEFCTPNIDGPN
AESVSHDQTLHSFHALFCRRCFKYDCFLHRLSTHPGPNLQKRKGPEVESSGEPCGPDCYL
HIVPKQTEPSPKQQDQRQKAIDRSKFTLESSPSPMEADDVWTGSDQSFFRVLHRCFLDNY
CAIAKGMQTKTCRQVYEFAQEELQHTEPLELGKHASPPRKQQKKKINTKLWTMHIRKNAR
KEANANNVYKYTPCDHSGPCGAGCCCFDSSNYCEKFCNCSSDCANRFPGCRCKAQCNTKQ
CSCYLAVRECDPDLCQCGAHQFHLNKMTCKNVSVQRGLRKHLLMAPSDIAGWGIFLKDGA
QKNEFISEYCGEIISQDEADRRGKVYDKYACSFLFNLNNDFVVDATRKGNKIRFANHSIN
PNCYAKVIMVNGDHRIGIFAKRIIQPGEELFFDYRYGPTEQLKFVGIEREMEIL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g16349.t1 | CDD | cd00167 | SANT | 341 | 383 | 0.00383162 |
| 13 | g16349.t1 | Coils | Coil | Coil | 14 | 34 | - |
| 12 | g16349.t1 | Coils | Coil | Coil | 37 | 57 | - |
| 11 | g16349.t1 | Gene3D | G3DSA:2.170.270.10 | SET domain | 431 | 637 | 6.4E-75 |
| 16 | g16349.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 306 | 333 | - |
| 5 | g16349.t1 | PANTHER | PTHR45747 | HISTONE-LYSINE N-METHYLTRANSFERASE E(Z) | 4 | 166 | 3.6E-229 |
| 8 | g16349.t1 | PANTHER | PTHR45747:SF1 | HISTONE-LYSINE N-METHYLTRANSFERASE EZH1 | 4 | 166 | 3.6E-229 |
| 4 | g16349.t1 | PANTHER | PTHR45747 | HISTONE-LYSINE N-METHYLTRANSFERASE E(Z) | 177 | 303 | 3.6E-229 |
| 7 | g16349.t1 | PANTHER | PTHR45747:SF1 | HISTONE-LYSINE N-METHYLTRANSFERASE EZH1 | 177 | 303 | 3.6E-229 |
| 6 | g16349.t1 | PANTHER | PTHR45747 | HISTONE-LYSINE N-METHYLTRANSFERASE E(Z) | 335 | 654 | 3.6E-229 |
| 9 | g16349.t1 | PANTHER | PTHR45747:SF1 | HISTONE-LYSINE N-METHYLTRANSFERASE EZH1 | 335 | 654 | 3.6E-229 |
| 3 | g16349.t1 | Pfam | PF18118 | Polycomb repressive complex 2 tri-helical domain | 183 | 220 | 2.4E-10 |
| 1 | g16349.t1 | Pfam | PF18264 | CXC domain | 469 | 499 | 1.4E-7 |
| 2 | g16349.t1 | Pfam | PF00856 | SET domain | 531 | 634 | 1.8E-17 |
| 17 | g16349.t1 | ProSiteProfiles | PS51633 | CXC domain profile. | 414 | 513 | 18.902 |
| 18 | g16349.t1 | ProSiteProfiles | PS50280 | SET domain profile. | 520 | 635 | 17.3 |
| 15 | g16349.t1 | SMART | SM00317 | set_7 | 520 | 641 | 1.9E-38 |
| 10 | g16349.t1 | SUPERFAMILY | SSF82199 | SET domain | 423 | 643 | 1.31E-60 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0070734 | histone H3-K27 methylation | BP |
| GO:0005515 | protein binding | MF |
| GO:0018024 | histone-lysine N-methyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K11430 ([histone H3]-lysine27 N-trimethyltransferase EZH2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
