General information for g16394.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin BII.
Parent gene
- The parent gene g16394 is located on chr_4: 9324359..9332549 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16394 | g16394.t10 | TSS | g16394.t10 | 9323582 | 9323582 |
| chr_4 | g16394 | g16394.t10 | isoform | g16394.t10 | 9323612 | 9324864 |
| chr_4 | g16394 | g16394.t10 | exon | g16394.t10.exon1 | 9323612 | 9324034 |
| chr_4 | g16394 | g16394.t10 | exon | g16394.t10.exon2 | 9324359 | 9324637 |
| chr_4 | g16394 | g16394.t10 | cds | g16394.t10.CDS1 | 9324381 | 9324637 |
| chr_4 | g16394 | g16394.t10 | exon | g16394.t10.exon3 | 9324699 | 9324864 |
| chr_4 | g16394 | g16394.t10 | cds | g16394.t10.CDS2 | 9324699 | 9324864 |
| chr_4 | g16394 | g16394.t10 | TTS | g16394.t10 | 9324913 | 9324913 |
Sequences
>g16394.t10 Gene=g16394 Length=868
ATGAAGTTCTTTCTTTTAACAATTTTTGCAATCGCTGTTGTGTCAGCAGTTGAAGATTAT
AATGCACCAGAATGGCAACCAATTGATTGGGCAAAAGTAATTCCTGCTGATGAGTTGCCA
GGTTTCTGGGAAAATACAGTTTTCGCTCAACTTGCACCAAGATCAAACAGAGAGCGCAGA
ATTGTTGGTGGTCATGAAGCAACACCACATGCTCATCCATATCAAGTCGCTCTGATTCAT
CAACGTGTAATCTTGGCTACAATGTGTGGTGGTTCAGTTATTAGTCCAACTGTTATTCTC
ACAGCTGCTCATTGTCCAATTGGTTCACAATCATCAACTGTCATCACTGGAGCACATAAT
ATGAATGTAATTGAACCAAATCAACAACGTAGAACTGTTCAATCAGCACAATATCGTATT
CATTTTGAATAACGATATCGCTACCATGATCCTTTTGCAACCATTGACACTCAACCAATT
TGTTGCTGTTATTCCATTGGCACAAGCGAATGCTGGAACATTTGCAGGAGTGACTGGACA
ATCCATTGGATGGGGAAGAACAGTTGATGGTGGTGCTTCGTCACCAGTTTTGCGAGTTGT
TCAAAATCCAATTATCACAAATGCTGTTTGTGCACAAACCTTTGGAACTGCTGTTGTAAA
TGCAGCTGTTATATGCACTGATACTACTGGTGGACGTGGAACATGTCAAGGTGATTCGGG
TGGAGTTCTTTCAGTATCGCAAGGTTCAGGACGTCTTCAAGTTGGTATTACGAGTTTTGG
CAGCAGTGCAGGATGTCAAGCAGGCTTTCCAGCTGGCTATGAAAGAGTGAGTGCACAAAT
CGGATGGATTAACAGCAATATGTCATAA
>g16394.t10 Gene=g16394 Length=140
MILLQPLTLNQFVAVIPLAQANAGTFAGVTGQSIGWGRTVDGGASSPVLRVVQNPIITNA
VCAQTFGTAVVNAAVICTDTTGGRGTCQGDSGGVLSVSQGSGRLQVGITSFGSSAGCQAG
FPAGYERVSAQIGWINSNMS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g16394.t10 | Gene3D | G3DSA:2.40.10.10 | - | 3 | 135 | 1.7E-29 |
| 6 | g16394.t10 | Gene3D | G3DSA:2.40.10.10 | - | 16 | 129 | 1.7E-29 |
| 2 | g16394.t10 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 3 | 137 | 9.3E-39 |
| 3 | g16394.t10 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 3 | 137 | 9.3E-39 |
| 1 | g16394.t10 | Pfam | PF00089 | Trypsin | 5 | 135 | 1.3E-22 |
| 8 | g16394.t10 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 85 | 96 | - |
| 10 | g16394.t10 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 1 | 140 | 15.111 |
| 9 | g16394.t10 | SMART | SM00020 | trypsin_2 | 1 | 135 | 4.1E-6 |
| 4 | g16394.t10 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 3 | 139 | 3.72E-32 |
| 5 | g16394.t10 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed