General information for g16395.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Brachyurin.
Parent gene
- The parent gene g16395 is located on chr_4: 9326800..9327163 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16395 | g16395.t1 | TTS | g16395.t1 | 9326087 | 9326087 |
| chr_4 | g16395 | g16395.t1 | isoform | g16395.t1 | 9326158 | 9327163 |
| chr_4 | g16395 | g16395.t1 | exon | g16395.t1.exon1 | 9326158 | 9326329 |
| chr_4 | g16395 | g16395.t1 | cds | g16395.t1.CDS1 | 9326158 | 9326329 |
| chr_4 | g16395 | g16395.t1 | exon | g16395.t1.exon2 | 9326384 | 9326412 |
| chr_4 | g16395 | g16395.t1 | cds | g16395.t1.CDS2 | 9326384 | 9326412 |
| chr_4 | g16395 | g16395.t1 | exon | g16395.t1.exon3 | 9326499 | 9326537 |
| chr_4 | g16395 | g16395.t1 | cds | g16395.t1.CDS3 | 9326499 | 9326537 |
| chr_4 | g16395 | g16395.t1 | exon | g16395.t1.exon4 | 9326598 | 9326725 |
| chr_4 | g16395 | g16395.t1 | cds | g16395.t1.CDS4 | 9326598 | 9326725 |
| chr_4 | g16395 | g16395.t1 | exon | g16395.t1.exon5 | 9326789 | 9326939 |
| chr_4 | g16395 | g16395.t1 | cds | g16395.t1.CDS5 | 9326789 | 9326939 |
| chr_4 | g16395 | g16395.t1 | exon | g16395.t1.exon6 | 9327008 | 9327163 |
| chr_4 | g16395 | g16395.t1 | cds | g16395.t1.CDS6 | 9327008 | 9327163 |
| chr_4 | g16395 | g16395.t1 | TSS | g16395.t1 | NA | NA |
Sequences
>g16395.t1 Gene=g16395 Length=675
ATGAAGTCACCAGTCAATGTCCGTGATCGTCGTATTGTCGGTGGTGTGAAGTTGTTCCAC
ACAGCCATCCATACCAAGTTGCCCTTCACATTCAATTCGGTGGTGGTACTGGTTCTCTGC
GGTGGTTCAGTTTTGACAATCCGTTCAGTTTTGACTGCTGCTCATTGTCCAATTGGTTCA
AGCTCAACCTTGGTCATTGCTGGTGCTCACAACAGAAATGTCGTTGAAGCTAATCAACAA
CGTCGTACTGTTCCATCATCAGGCTACCGCATCCACGCCAACTACAATCCATCAAACTTG
AACAATGATATTGCTGTTCTCATCACACCAACACCAAACTTTGCATGGACCACAGCTGGT
CAACCAACACGTCGTCCAACTGGTGCTCAACTTAGCGATCTTTTCGTTGGAGATAGATCA
CGTGCAACTGGCTGGGGTCGTGTTACCAACACTGGGCAACATCAGCTGTCCTCCGAAACA
GCTCAATCAAATCAAGGAACATGCAACGGAGATTCAGGCGGTGTGCTTTCAGTTGAACGC
CCTGGACACGCTCAATGGGTCCAAGTTGGTGTTACCTCATTTGGTGCTGCTGCTGGTTGT
GTTGCTGGATTCCCATCAGGCTTCGCTCGTATGACTCATTTCGATGCATGGATTAATGCC
AACCAAAATCCATAA
>g16395.t1 Gene=g16395 Length=224
MKSPVNVRDRRIVGGVKLFHTAIHTKLPFTFNSVVVLVLCGGSVLTIRSVLTAAHCPIGS
SSTLVIAGAHNRNVVEANQQRRTVPSSGYRIHANYNPSNLNNDIAVLITPTPNFAWTTAG
QPTRRPTGAQLSDLFVGDRSRATGWGRVTNTGQHQLSSETAQSNQGTCNGDSGGVLSVER
PGHAQWVQVGVTSFGAAAGCVAGFPSGFARMTHFDAWINANQNP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g16395.t1 | Gene3D | G3DSA:2.40.10.10 | - | 38 | 122 | 5.4E-20 |
| 12 | g16395.t1 | Gene3D | G3DSA:2.40.10.10 | - | 123 | 154 | 5.4E-20 |
| 13 | g16395.t1 | Gene3D | G3DSA:2.40.10.10 | - | 162 | 212 | 2.2E-11 |
| 4 | g16395.t1 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 9 | 152 | 9.1E-41 |
| 6 | g16395.t1 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 9 | 152 | 9.1E-41 |
| 3 | g16395.t1 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 162 | 220 | 9.1E-41 |
| 5 | g16395.t1 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 162 | 220 | 9.1E-41 |
| 9 | g16395.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 41 | 56 | 2.4E-8 |
| 8 | g16395.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 99 | 113 | 2.4E-8 |
| 7 | g16395.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 165 | 177 | 2.4E-8 |
| 1 | g16395.t1 | Pfam | PF00089 | Trypsin | 38 | 156 | 8.9E-19 |
| 2 | g16395.t1 | Pfam | PF00089 | Trypsin | 160 | 218 | 5.6E-6 |
| 14 | g16395.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 51 | 56 | - |
| 15 | g16395.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 166 | 177 | - |
| 17 | g16395.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 12 | 223 | 17.486 |
| 16 | g16395.t1 | SMART | SM00020 | trypsin_2 | 11 | 218 | 1.6E-23 |
| 10 | g16395.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 9 | 221 | 3.21E-38 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed