General information for g16397.t25
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin BII.
Parent gene
- The parent gene g16397 is located on chr_4: 9336657..9342138 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16397 | g16397.t25 | TTS | g16397.t25 | 9326087 | 9326087 |
| chr_4 | g16397 | g16397.t25 | isoform | g16397.t25 | 9336128 | 9337367 |
| chr_4 | g16397 | g16397.t25 | exon | g16397.t25.exon1 | 9336128 | 9336380 |
| chr_4 | g16397 | g16397.t25 | cds | g16397.t25.CDS1 | 9336209 | 9336380 |
| chr_4 | g16397 | g16397.t25 | exon | g16397.t25.exon2 | 9336435 | 9336589 |
| chr_4 | g16397 | g16397.t25 | cds | g16397.t25.CDS2 | 9336435 | 9336589 |
| chr_4 | g16397 | g16397.t25 | exon | g16397.t25.exon3 | 9336649 | 9336776 |
| chr_4 | g16397 | g16397.t25 | cds | g16397.t25.CDS3 | 9336649 | 9336776 |
| chr_4 | g16397 | g16397.t25 | exon | g16397.t25.exon4 | 9336840 | 9336975 |
| chr_4 | g16397 | g16397.t25 | cds | g16397.t25.CDS4 | 9336840 | 9336975 |
| chr_4 | g16397 | g16397.t25 | exon | g16397.t25.exon5 | 9337059 | 9337367 |
| chr_4 | g16397 | g16397.t25 | cds | g16397.t25.CDS5 | 9337059 | 9337367 |
| chr_4 | g16397 | g16397.t25 | TSS | g16397.t25 | 9337406 | 9337406 |
Sequences
>g16397.t25 Gene=g16397 Length=981
ATGAAACTTTTCCTTCTCGCGATCCTCGCCGTTGGCGCTCTTGCCCAGGAGAACTATGAG
GGCCCAGAGTACGCTCCAATTGATCCATCATCAATCATTCCTGTTCAGGAAATGCCCGGT
TTCTGGGATGACAAGACCGAATTCCCAGCATGGATGAAGTCACCAGTCAATGTCCGTGAT
CGTCGTATTGTCGGTGGTGTTGAAGTTGTTCCACACAGCCATCCATACCAAGTTGCCCTT
CACATTCAATTCGGTGGTGGTACTGGTCTCTGCGGTGGTTCAGTTTTGACAATCCGTTCA
GTTTTGACTATTGGTTCAAGCTCAACCTTGGTCATTGCTGGTGCTCACAACAGAAATGTC
GTTGAAGCCAATCAACAACGTCGTACTGTTCCATCATCAGGCTACCGCATCCACGCCAAC
TACAATCCATCAAACTTGAACAATGATATTGCTGTTCTCATCACACCAACACCAAACTTT
GCATGGACCACAGCTGTTCAACCAACACGTCGTCCAACTGGTGCTCAACTTAGCGATCTT
TTCGTTGGAGATAGATCACGTGCAACTGGCTGGGGTCGTGTTACCAACACTGGTGCAACA
TCAGCTGTCCTCCGTAAGGCTTACAATACAATCATCACTAATGCTGCTTGCGCTCAAGTT
TATGGAACAAGCGTCGTAAATGCTGGAGTTATTTGCAAGGAAACAGCTCAATCAAATCAA
GGAACATGCAACGGAGATTCAGGCGGTGTGCTTTCAGTTGAACGTCCTGGACACGCTCAA
TGGGTCCAAGTTGGTGTTACCTCATTTGGTGCTGCTGCTGGTTGTGTTGCTGGATTCCCA
TCAGGCTTCGCTCGTATGACTCATTTCGATGCATGGATTAATGCCAACCAAAATCCATAA
AAAGTGAACATTCATTTTTAAATGGAATGAAGATCAAAAAAAAAATTGTTAAATTAAATT
CGAAATTTTTGCAAAACAAAA
>g16397.t25 Gene=g16397 Length=299
MKLFLLAILAVGALAQENYEGPEYAPIDPSSIIPVQEMPGFWDDKTEFPAWMKSPVNVRD
RRIVGGVEVVPHSHPYQVALHIQFGGGTGLCGGSVLTIRSVLTIGSSSTLVIAGAHNRNV
VEANQQRRTVPSSGYRIHANYNPSNLNNDIAVLITPTPNFAWTTAVQPTRRPTGAQLSDL
FVGDRSRATGWGRVTNTGATSAVLRKAYNTIITNAACAQVYGTSVVNAGVICKETAQSNQ
GTCNGDSGGVLSVERPGHAQWVQVGVTSFGAAAGCVAGFPSGFARMTHFDAWINANQNP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g16397.t25 | CDD | cd00190 | Tryp_SPc | 63 | 296 | 8.06249E-50 |
| 6 | g16397.t25 | Gene3D | G3DSA:2.40.10.10 | - | 57 | 298 | 8.1E-45 |
| 2 | g16397.t25 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 58 | 295 | 3.9E-62 |
| 3 | g16397.t25 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 58 | 295 | 3.9E-62 |
| 1 | g16397.t25 | Pfam | PF00089 | Trypsin | 63 | 293 | 1.8E-35 |
| 8 | g16397.t25 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 15 | - |
| 9 | g16397.t25 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 10 | g16397.t25 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 11 | g16397.t25 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 15 | - |
| 7 | g16397.t25 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 16 | 299 | - |
| 13 | g16397.t25 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 241 | 252 | - |
| 15 | g16397.t25 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 63 | 298 | 22.872 |
| 14 | g16397.t25 | SMART | SM00020 | trypsin_2 | 62 | 293 | 8.8E-44 |
| 4 | g16397.t25 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 55 | 296 | 2.65E-52 |
| 5 | g16397.t25 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 15 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed