General information for g16421.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glutathione S-transferase.
Parent gene
- The parent gene g16421 is located on chr_4: 9398962..9399536 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16421 | g16421.t2 | TSS | g16421.t2 | 9398580 | 9398580 |
| chr_4 | g16421 | g16421.t2 | isoform | g16421.t2 | 9398638 | 9399536 |
| chr_4 | g16421 | g16421.t2 | exon | g16421.t2.exon1 | 9398638 | 9398767 |
| chr_4 | g16421 | g16421.t2 | cds | g16421.t2.CDS1 | 9398638 | 9398767 |
| chr_4 | g16421 | g16421.t2 | exon | g16421.t2.exon2 | 9398974 | 9399210 |
| chr_4 | g16421 | g16421.t2 | cds | g16421.t2.CDS2 | 9398974 | 9399210 |
| chr_4 | g16421 | g16421.t2 | exon | g16421.t2.exon3 | 9399356 | 9399536 |
| chr_4 | g16421 | g16421.t2 | cds | g16421.t2.CDS3 | 9399356 | 9399402 |
| chr_4 | g16421 | g16421.t2 | TTS | g16421.t2 | 9399585 | 9399585 |
Sequences
>g16421.t2 Gene=g16421 Length=548
ATGCCATATAAACTTCAATATTTTAACGCTCGTGGAACAGCTGAGCCAATTCGTTATTTA
CTTAAATATGGAAAAATCAACTTTGAAGATGTGAGGATTGAATCAAGTGATTGGCCTAAA
ATTAAAGCAACAATGCCAATGGGTCAAATGCCAATTCTTGAATGTGACGGCAAAAGATTC
TTTCAATCTGTTGCCATTTGTCGCTTTTTAGCTAAAAAAGTTGGACTAGCTGGCAGTAAT
GAATTGGAAAATCTTGAAATTGACAGTATTGCTGATACTGTTGTTGATTTGAGATTGAGT
AAGTTTATGAGAATAAATTGGAATTTAATTTTAATATATTTGTTCATTGTTCATTTGCAT
GATTGCTCAAGAAACTCACTTGGGCTGATTTCTATTTTGCTGCATTTGTCATAATGTTTC
AATTTGGCGCACAAGAAGGTGAAAAAGTTTGGGAAAAATATCCAGCATTGAAGAAAGTCA
AAGAAAATGTTGAGAATATTGATGAAATTAAAAAGTGGATTGCTGTTCGTCCTGTAACCG
ATCTTTGA
>g16421.t2 Gene=g16421 Length=137
MPYKLQYFNARGTAEPIRYLLKYGKINFEDVRIESSDWPKIKATMPMGQMPILECDGKRF
FQSVAICRFLAKKVGLAGSNELENLEIDSIADTVVDLRLSKFMRINWNLILIYLFIVHLH
DCSRNSLGLISILLHLS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g16421.t2 | CDD | cd03039 | GST_N_Sigma_like | 3 | 72 | 0.000 |
| 5 | g16421.t2 | Gene3D | G3DSA:1.20.1050.130 | - | 1 | 110 | 0.000 |
| 2 | g16421.t2 | PANTHER | PTHR11571:SF234 | GLUTATHIONE S-TRANSFERASE S1 | 3 | 100 | 0.000 |
| 3 | g16421.t2 | PANTHER | PTHR11571 | GLUTATHIONE S-TRANSFERASE | 3 | 100 | 0.000 |
| 1 | g16421.t2 | Pfam | PF02798 | Glutathione S-transferase, N-terminal domain | 4 | 71 | 0.000 |
| 6 | g16421.t2 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 1 | 78 | 27.506 |
| 8 | g16421.t2 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 3 | 109 | 0.000 |
| 4 | g16421.t2 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 1 | 75 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed