General information for g16421.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glutathione S-transferase.
Parent gene
- The parent gene g16421 is located on chr_4: 9398962..9399536 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16421 | g16421.t3 | TSS | g16421.t3 | 9398580 | 9398580 |
| chr_4 | g16421 | g16421.t3 | isoform | g16421.t3 | 9398962 | 9399536 |
| chr_4 | g16421 | g16421.t3 | exon | g16421.t3.exon1 | 9398962 | 9399141 |
| chr_4 | g16421 | g16421.t3 | cds | g16421.t3.CDS1 | 9398976 | 9399141 |
| chr_4 | g16421 | g16421.t3 | exon | g16421.t3.exon2 | 9399223 | 9399536 |
| chr_4 | g16421 | g16421.t3 | cds | g16421.t3.CDS2 | 9399223 | 9399536 |
| chr_4 | g16421 | g16421.t3 | TTS | g16421.t3 | 9399585 | 9399585 |
Sequences
>g16421.t3 Gene=g16421 Length=494
TTAAAATTTCAGCAATGCCAATGGGTCAAATGCCAATTCTTGAATGTGACGGCAAAAGAT
TCTTTCAATCTGTTGCCATTTGTCGCTTTTTAGCTAAAAAAGTTGGACTAGCTGGCAGTA
ATGAATTGGAAAATCTTGAAATTGACAGTATTGCTGATACTGTTGTTGATTTGAGATTGA
AAATTATGGCAGTTTCATTTGGTCCACAAGATAAAAAAGCTGAAGGAATGAAAACTCTAA
AAGAAGAAACAGTTCCATTTTTCTTATCAAAACTAAATGCGATTGCTGAAGAAAACAATG
GACATTTGGCATGCAAGAAACTCACTTGGGCTGATTTCTATTTTGCTGCATTTGTCATAA
TGTTTCAATTTGGCGCACAAGAAGGTGAAAAAGTTTGGGAAAAATATCCAGCATTGAAGA
AAGTCAAAGAAAATGTTGAGAATATTGATGAAATTAAAAAGTGGATTGCTGTTCGTCCTG
TAACCGATCTTTGA
>g16421.t3 Gene=g16421 Length=159
MPMGQMPILECDGKRFFQSVAICRFLAKKVGLAGSNELENLEIDSIADTVVDLRLKIMAV
SFGPQDKKAEGMKTLKEETVPFFLSKLNAIAEENNGHLACKKLTWADFYFAAFVIMFQFG
AQEGEKVWEKYPALKKVKENVENIDEIKKWIAVRPVTDL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g16421.t3 | CDD | cd03192 | GST_C_Sigma_like | 39 | 141 | 0.0000000 |
| 7 | g16421.t3 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 31 | 0.0000000 |
| 6 | g16421.t3 | Gene3D | G3DSA:1.20.1050.10 | - | 32 | 143 | 0.0000000 |
| 2 | g16421.t3 | PANTHER | PTHR11571:SF234 | GLUTATHIONE S-TRANSFERASE S1 | 1 | 159 | 0.0000000 |
| 3 | g16421.t3 | PANTHER | PTHR11571 | GLUTATHIONE S-TRANSFERASE | 1 | 159 | 0.0000000 |
| 1 | g16421.t3 | Pfam | PF14497 | Glutathione S-transferase, C-terminal domain | 52 | 152 | 0.0000000 |
| 9 | g16421.t3 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 1 | 34 | 14.9660000 |
| 8 | g16421.t3 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 36 | 159 | 17.6430000 |
| 11 | g16421.t3 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 1 | 141 | 0.0000000 |
| 4 | g16421.t3 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 1 | 31 | 0.0000005 |
| 5 | g16421.t3 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 32 | 158 | 0.0000000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
