Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Glutathione S-transferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16424 g16424.t1 TSS g16424.t1 9405269 9405269
chr_4 g16424 g16424.t1 isoform g16424.t1 9405376 9406102
chr_4 g16424 g16424.t1 exon g16424.t1.exon1 9405376 9405478
chr_4 g16424 g16424.t1 cds g16424.t1.CDS1 9405376 9405478
chr_4 g16424 g16424.t1 exon g16424.t1.exon2 9405532 9405699
chr_4 g16424 g16424.t1 cds g16424.t1.CDS2 9405532 9405699
chr_4 g16424 g16424.t1 exon g16424.t1.exon3 9405792 9406102
chr_4 g16424 g16424.t1 cds g16424.t1.CDS3 9405792 9406102
chr_4 g16424 g16424.t1 TTS g16424.t1 9406134 9406134

Sequences

>g16424.t1 Gene=g16424 Length=582
ATGGGCTTAGCTGAGCCAATAAGATTTCTTTTTCACTATGGAAGCATTGACTTTATTGAT
AATCGCTTAAATAAAGAAAAAGATTGGCCAAAATTTAAAGAAACAACTCCATATGGTCAA
GTACCATTACTTGAATTTGATGGAAAAGTAATCAACCAATCTCGTGCAATATGTCGATTT
TTAGCATCAAAAGTTGATCTTGATGGAAAGAATGACATGGAAAATCTTGAAATTGATAGC
GTTGTTGATACCATTTATGACTTGCGATTAAAAATTCGAGAAGTTTTTCATGAAATGAAT
CAAGAAGTTAAAAATGAAAAGCGAAAAGTTCTTCACTCAACACATATTCCTTTCTATATT
TCATTACTTGATAAACTTGCTGCAGAAAATAATGGTCATTTGGCTTTAAATCGTCTTACA
TGGGCTGATTTGTTTTTCGCTGCTGTCAAAATTTATTTTAGTCACTGCGCTGAATATGAT
GTTCTTCAAAATGCACCAAATTTGATAAAAGTTGTTGAAAATGTTGAAAAAATTGAAAAC
ATCAAAAAATGGATTGAGAAACGTCCCAAAACTAGTTTTTGA

>g16424.t1 Gene=g16424 Length=193
MGLAEPIRFLFHYGSIDFIDNRLNKEKDWPKFKETTPYGQVPLLEFDGKVINQSRAICRF
LASKVDLDGKNDMENLEIDSVVDTIYDLRLKIREVFHEMNQEVKNEKRKVLHSTHIPFYI
SLLDKLAAENNGHLALNRLTWADLFFAAVKIYFSHCAEYDVLQNAPNLIKVVENVEKIEN
IKKWIEKRPKTSF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
12 g16424.t1 CDD cd03039 GST_N_Sigma_like 1 62 0.000
11 g16424.t1 CDD cd03192 GST_C_Sigma_like 73 175 0.000
8 g16424.t1 Gene3D G3DSA:3.40.30.10 Glutaredoxin 2 187 0.000
7 g16424.t1 Gene3D G3DSA:1.20.1050.10 - 66 177 0.000
3 g16424.t1 PANTHER PTHR11571:SF234 GLUTATHIONE S-TRANSFERASE S1 2 192 0.000
4 g16424.t1 PANTHER PTHR11571 GLUTATHIONE S-TRANSFERASE 2 192 0.000
1 g16424.t1 Pfam PF02798 Glutathione S-transferase, N-terminal domain 18 62 0.000
2 g16424.t1 Pfam PF14497 Glutathione S-transferase, C-terminal domain 87 188 0.000
10 g16424.t1 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 1 69 22.484
9 g16424.t1 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 71 193 16.463
13 g16424.t1 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 2 175 0.000
5 g16424.t1 SUPERFAMILY SSF52833 Thioredoxin-like 2 65 0.000
6 g16424.t1 SUPERFAMILY SSF47616 GST C-terminal domain-like 67 193 0.000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values