General information for g16433.t14
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase/endoribonuclease IRE1.
Parent gene
- The parent gene g16433 is located on chr_4: 9426103..9426659 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16433 | g16433.t14 | TTS | g16433.t14 | 9422557 | 9422557 |
| chr_4 | g16433 | g16433.t14 | isoform | g16433.t14 | 9422579 | 9424199 |
| chr_4 | g16433 | g16433.t14 | exon | g16433.t14.exon1 | 9422579 | 9422980 |
| chr_4 | g16433 | g16433.t14 | cds | g16433.t14.CDS1 | 9422579 | 9422980 |
| chr_4 | g16433 | g16433.t14 | exon | g16433.t14.exon2 | 9423048 | 9423515 |
| chr_4 | g16433 | g16433.t14 | cds | g16433.t14.CDS2 | 9423048 | 9423515 |
| chr_4 | g16433 | g16433.t14 | exon | g16433.t14.exon3 | 9423574 | 9423612 |
| chr_4 | g16433 | g16433.t14 | cds | g16433.t14.CDS3 | 9423574 | 9423612 |
| chr_4 | g16433 | g16433.t14 | exon | g16433.t14.exon4 | 9423670 | 9424199 |
| chr_4 | g16433 | g16433.t14 | cds | g16433.t14.CDS4 | 9423670 | 9423753 |
| chr_4 | g16433 | g16433.t14 | TSS | g16433.t14 | NA | NA |
Sequences
>g16433.t14 Gene=g16433 Length=1439
ATGGACGAACAGTGATATCAAAAATAAAATTTCATCGCAATGATCTCATTGGTCGTGGAA
GTGAAGGAACTTCAGTATTTAAAGGCACGTTTGAAGGTCGTGAAGTTGCAGTCAAACGTC
TTCTTACCAGTAATTTTTCACTTGCTGAACGCGAAGTTAAAATTCTTCAAAAATCAGATG
CAAATGAAAACATCATCCGATATTTTTGCACAGAATTTGATCGTGAATTTTGCTACATTG
CAATTGAACTTTGTGCATGTTCACTTTATGATTTTACAGTAAATGAAGATTTTCAATATT
TGAAAGATTTAATCAAACCAAAAGAAGTTCTTTATCAAGCTACAAAAGGATTGTCTTATT
TACATCAATTAGGGATTATTCATAGAGATATAAAACCACAGAATATTTTAATTTCTAAAC
CAGATGGTAGAAAAAATATTAAAGTCATGATTTCTGATTTTGGTCTTTGTAAAAAATTTT
CTAAAGGAGAAATTTCTGTAACAAATAGAATTGGAATTGCAGGAACTGAGGGTTGGATTG
CACCTGAAATTCTCATGAATGAAAAATCGTCAAAAATCGCAGATATTTTTTCACTTGGTT
GTGTTTTTTATTTCACACTCACAAATGGTCAACATCCTTTTGGTGATTCATTAACAAGAA
CTTCAAACATTCTTGCCAATAATTTCAATCTTAGTCATTTAAATGACAGCAATTATGAAG
AAATTTTTGCTGCAGAACTTATAAGTGACATGATAAATGTCAATACAAAGATACGACCAG
TTGCTGATGCAATTATAAGACATCCATTATTTTGGAGTGAAGGAAAAATGTTGGATTTTC
TTCATAGTGTTTCTGATAAAGTTGAAAAATTAAATACAACTGAAGATCCACTTTGGTCAC
TTGAAAGAAATGCAAAAATGATTGTTAGAGAAGATTGGAAGAAAATTATTGATGAAGAAA
TTTTAAATGATTTAGGAAAGCAAAGAACTTATCATGGAATTAGTGTGAGAGATCTTTTAA
GAGCTATAAGAAATAAACGCAATCATTACATGGAACTGACAGAAGAAGTTAAAAATATTC
TCGGATCAATTCCAGTTGATTACTTAAATTATTGGCTATCAAAATTTCCTCATTTAGCTC
CACATGCTTATCATGTAATGTCATCAAATCAAAAAGATTCAACATTTTCAAGATTTTATG
TTCGTTATCATCTTTTTACAAAACCTAGTTACATATCAGACACAAATCTTGACAATCATG
AATTGGTTGAATTACAAGCAAAATGTAGAGCTGAAAATGCAAATTCTAAATTTAATAACA
ATCAAAACTTTCAAAACCATGGAAGTCCAAATGGAAGAAAATTCTACAATAACAATAATT
GGAATAAAAATAAAAATAGCAAACGAGGATTTTATAATTTTAAAAATAATGAAACGTGA
>g16433.t14 Gene=g16433 Length=330
MISDFGLCKKFSKGEISVTNRIGIAGTEGWIAPEILMNEKSSKIADIFSLGCVFYFTLTN
GQHPFGDSLTRTSNILANNFNLSHLNDSNYEEIFAAELISDMINVNTKIRPVADAIIRHP
LFWSEGKMLDFLHSVSDKVEKLNTTEDPLWSLERNAKMIVREDWKKIIDEEILNDLGKQR
TYHGISVRDLLRAIRNKRNHYMELTEEVKNILGSIPVDYLNYWLSKFPHLAPHAYHVMSS
NQKDSTFSRFYVRYHLFTKPSYISDTNLDNHELVELQAKCRAENANSKFNNNQNFQNHGS
PNGRKFYNNNNWNKNKNSKRGFYNFKNNET
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g16433.t14 | Coils | Coil | Coil | 187 | 207 | - |
| 7 | g16433.t14 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 124 | 1.1E-26 |
| 8 | g16433.t14 | Gene3D | G3DSA:1.20.1440.180 | - | 126 | 266 | 2.5E-43 |
| 3 | g16433.t14 | PANTHER | PTHR13954:SF15 | SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE2 | 1 | 255 | 2.9E-82 |
| 4 | g16433.t14 | PANTHER | PTHR13954 | IRE1-RELATED | 1 | 255 | 2.9E-82 |
| 2 | g16433.t14 | Pfam | PF00069 | Protein kinase domain | 2 | 122 | 4.4E-15 |
| 1 | g16433.t14 | Pfam | PF06479 | Ribonuclease 2-5A | 129 | 251 | 8.3E-37 |
| 11 | g16433.t14 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 122 | 18.308 |
| 10 | g16433.t14 | ProSiteProfiles | PS51392 | KEN domain profile. | 125 | 253 | 29.968 |
| 6 | g16433.t14 | SMART | SM00580 | PGNneu | 185 | 239 | 1.1E-11 |
| 5 | g16433.t14 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 2 | 128 | 2.82E-22 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006397 | mRNA processing | BP |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0004540 | ribonuclease activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
