General information for g16433.t16
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine/threonine-protein kinase/endoribonuclease IRE1.
Parent gene
- The parent gene g16433 is located on chr_4: 9426103..9426659 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16433 | g16433.t16 | isoform | g16433.t16 | 9424270 | 9426659 |
| chr_4 | g16433 | g16433.t16 | exon | g16433.t16.exon1 | 9424270 | 9424673 |
| chr_4 | g16433 | g16433.t16 | cds | g16433.t16.CDS1 | 9424271 | 9424673 |
| chr_4 | g16433 | g16433.t16 | exon | g16433.t16.exon2 | 9424841 | 9425066 |
| chr_4 | g16433 | g16433.t16 | cds | g16433.t16.CDS2 | 9424841 | 9425066 |
| chr_4 | g16433 | g16433.t16 | exon | g16433.t16.exon3 | 9425804 | 9425925 |
| chr_4 | g16433 | g16433.t16 | cds | g16433.t16.CDS3 | 9425804 | 9425925 |
| chr_4 | g16433 | g16433.t16 | exon | g16433.t16.exon4 | 9426017 | 9426092 |
| chr_4 | g16433 | g16433.t16 | cds | g16433.t16.CDS4 | 9426017 | 9426092 |
| chr_4 | g16433 | g16433.t16 | exon | g16433.t16.exon5 | 9426157 | 9426523 |
| chr_4 | g16433 | g16433.t16 | cds | g16433.t16.CDS5 | 9426157 | 9426523 |
| chr_4 | g16433 | g16433.t16 | exon | g16433.t16.exon6 | 9426582 | 9426659 |
| chr_4 | g16433 | g16433.t16 | cds | g16433.t16.CDS6 | 9426582 | 9426659 |
| chr_4 | g16433 | g16433.t16 | TSS | g16433.t16 | 9426727 | 9426727 |
| chr_4 | g16433 | g16433.t16 | TTS | g16433.t16 | NA | NA |
Sequences
>g16433.t16 Gene=g16433 Length=1273
ATGAAAATCAATTTCAAGTTTTTAATTTTTATTTTTATCTTTCAATGGATAAATTTGGGT
AAAACTGATGATGATTTGAGAAAAATCGCAAGATCAGATGAACCATTAATGGTTCTTGTG
ACACTCTCAGGATCAATGATTGGAATCTGTCCACAAACAGCAACACAGAGATGGATAAAA
CAACATGATCCAATTGTCAAAACAAATGGAAATGAAAATTTAAATTTTTCAACAATTTTA
TTACCTGATCCAGTGACTGGAAAATTGTATAACATGAAAACTAGCGATAAAGACAAATAT
GAACTTTCACAAATGGATTTTACAATTCCTGACTTGGTACTTAAAACTCCTTTTATTTCT
AAGGATAAAATTTTATACACTGGAAGAAAATTGGATTCTTGGTTTATGATTAACTCACTA
ACTGGTGAATGCAAGACTGTTATGGATTTTGATGATAACAAAGATAAAAAGACAACAGAT
TTAATACCATTTCCAACAATTCATGATGTCTACATAGGAAAAACACAATATGATGTTTGT
ATGACAAATATGTACCCTGATAACAAAACTAGAGAAATATGGAATGTTTCCATGTATCAG
TACAATGCATTTGAAATGAATAAAGAAATTTATGACAAATATGAATTCAATCACATTGTC
AGCAGTTCAAGTGGGAAAATTCTAACACTTTTTAAAACAACAGGAAAAATTTTCTGGCAT
AAAACAGATTTGAATTCACCAGTCGTTGCCTTATTTATGATGGGTAGAAACGGATTTTTA
AACATTCCATTTACTACAGTTGATGATTCAGTATTTGAAAAGCTTTTGGAGCCTGTTGAT
TATGATAAATTAAAAGATATTCAATTACTTGAAACAATTTTTATCGGTGAAAATAAAAGT
GCAGGCTCAAATTCATTTTATGCTTTACCAACATTATCAGACAATACAACATTTATAATT
CGTGACAAAGTGATAAATTTTTTAAAAGATCCTTCATCAATTATTGGTCATTATTTATTA
CCATCATCAAATTCAGCTGAAACAAATTCAAAATTAATCACTGATGAATCACAAAGCAAT
AAATCTACAGAACTAAAAAATGAAAACAAAAATGAAAAAATCGCAGTGATTTTAGCATTG
GTTGTAATTTTAATTTCCTTGGTGATAACTTTTGGCATAATTGTGATAAAAAGAAAATTA
AAAAATGAAACAAAAAAGAATAATGTCAGTGAAAGCGATGAACAAATCGTCGTGGATTCA
AAAATAGATGAAG
>g16433.t16 Gene=g16433 Length=424
MKINFKFLIFIFIFQWINLGKTDDDLRKIARSDEPLMVLVTLSGSMIGICPQTATQRWIK
QHDPIVKTNGNENLNFSTILLPDPVTGKLYNMKTSDKDKYELSQMDFTIPDLVLKTPFIS
KDKILYTGRKLDSWFMINSLTGECKTVMDFDDNKDKKTTDLIPFPTIHDVYIGKTQYDVC
MTNMYPDNKTREIWNVSMYQYNAFEMNKEIYDKYEFNHIVSSSSGKILTLFKTTGKIFWH
KTDLNSPVVALFMMGRNGFLNIPFTTVDDSVFEKLLEPVDYDKLKDIQLLETIFIGENKS
AGSNSFYALPTLSDNTTFIIRDKVINFLKDPSSIIGHYLLPSSNSAETNSKLITDESQSN
KSTELKNENKNEKIAVILALVVILISLVITFGIIVIKRKLKNETKKNNVSESDEQIVVDS
KIDE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 1 | g16433.t16 | PANTHER | PTHR13954:SF15 | SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE2 | 30 | 281 | 4.8E-35 |
| 2 | g16433.t16 | PANTHER | PTHR13954 | IRE1-RELATED | 30 | 281 | 4.8E-35 |
| 7 | g16433.t16 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 8 | g16433.t16 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 9 | g16433.t16 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 17 | - |
| 11 | g16433.t16 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 22 | - |
| 6 | g16433.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 373 | - |
| 10 | g16433.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 374 | 396 | - |
| 5 | g16433.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 397 | 424 | - |
| 4 | g16433.t16 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 3 | g16433.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 374 | 396 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
