General information for g16464.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g16464 is located on chr_4: 9599227..9600965 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16464 | g16464.t2 | isoform | g16464.t2 | 9599227 | 9600965 |
| chr_4 | g16464 | g16464.t2 | exon | g16464.t2.exon1 | 9599227 | 9599266 |
| chr_4 | g16464 | g16464.t2 | cds | g16464.t2.CDS1 | 9599227 | 9599266 |
| chr_4 | g16464 | g16464.t2 | exon | g16464.t2.exon2 | 9599926 | 9600727 |
| chr_4 | g16464 | g16464.t2 | cds | g16464.t2.CDS2 | 9599926 | 9600727 |
| chr_4 | g16464 | g16464.t2 | exon | g16464.t2.exon3 | 9600776 | 9600965 |
| chr_4 | g16464 | g16464.t2 | cds | g16464.t2.CDS3 | 9600776 | 9600965 |
| chr_4 | g16464 | g16464.t2 | TSS | g16464.t2 | NA | NA |
| chr_4 | g16464 | g16464.t2 | TTS | g16464.t2 | NA | NA |
Sequences
>g16464.t2 Gene=g16464 Length=1032
ATGCTTGGTCTATTCACTGGTAATGAGTGGAGAGGAGAGTATTTTGGAATTTGTGACACA
ATCAACATTCAAGGTTTTAATGAACTTTTTAATAAAGACGCAATAAGTGAAGATTTTGAA
ATTTTTCGTCGAAGTGAAATTTCAAAATTTCCTGACCCTGAAAGTCCTTTGTTTATTGTC
AGCATCGATGATAATTTTGATGAAATCAATTGGTCACTTGAAAATGGTTACAAAAATAAC
GATGATGAAGTTTTACCAATTCGTGCATTAAAACGTAATAAAATTGGTGCTGAAATGTTT
TTTACAAAAGAAAATGCAAGGAATACAAACATGTGGTTTTGTCGATTGATGGGACGAGGT
TATAAAATTTTTATTCATTTACGAAATGAAGTTCCAACATTTTTACATAAGAAACATTTT
CTTGAATACAATACAGACAGATCTTTTACGGTTTTAGCAAAAATTTATAAAACAAGTAAG
GATTTGCAAGGTTATTCACCAAAAGTGCGAGGTTGTTACTTTGAAGGTGAAAAAGTTTTG
AAATTTTTTAAATCTTACGCAAAAACTCTTTGTGATTTTGAATGCATGACAAATTACACC
TTGAAAATTTGTGGATGTGTGAAATTTTCAATGCCAAGAACACAAAATACGCAAATTTGT
GATTTAGATAAAGTTGAATGCTATAACAATGCAATGATTACCTGGCCAAATGTTAATGAT
GAAATTGGACTTAGTGATGCTTCATCAACTTGTGACTGTCTTGATTCATGCACAAATATT
GAATATGATGTTGAAATTGAGAAAACTAATGCTGTTTATTTACCAAGTCAACATGGATTT
CTGAAAACTTATTCATTTTTTACTGTGGAATTTCCATCACATATAATTGAGGAAAATATC
AATTTTATAGGTTATACATTTCAAAATTTTGTAGCAGACTGCGGTGGCTTAATTGGACTT
TTCTTGGGATTATCATTGCTTTCAATTTTTGAAGTTTTTTATCGAACATTGAATGCATTT
TTAAAATTGTAA
>g16464.t2 Gene=g16464 Length=343
MLGLFTGNEWRGEYFGICDTINIQGFNELFNKDAISEDFEIFRRSEISKFPDPESPLFIV
SIDDNFDEINWSLENGYKNNDDEVLPIRALKRNKIGAEMFFTKENARNTNMWFCRLMGRG
YKIFIHLRNEVPTFLHKKHFLEYNTDRSFTVLAKIYKTSKDLQGYSPKVRGCYFEGEKVL
KFFKSYAKTLCDFECMTNYTLKICGCVKFSMPRTQNTQICDLDKVECYNNAMITWPNVND
EIGLSDASSTCDCLDSCTNIEYDVEIEKTNAVYLPSQHGFLKTYSFFTVEFPSHIIEENI
NFIGYTFQNFVADCGGLIGLFLGLSLLSIFEVFYRTLNAFLKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g16464.t2 | Gene3D | G3DSA:1.10.287.820 | - | 170 | 257 | 1.9E-7 |
| 7 | g16464.t2 | Gene3D | G3DSA:1.10.287.770 | - | 279 | 342 | 5.8E-8 |
| 2 | g16464.t2 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 72 | 338 | 2.8E-27 |
| 3 | g16464.t2 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 72 | 338 | 2.8E-27 |
| 1 | g16464.t2 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 15 | 334 | 6.8E-41 |
| 9 | g16464.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 314 | - |
| 10 | g16464.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 315 | 334 | - |
| 8 | g16464.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 335 | 343 | - |
| 5 | g16464.t2 | ProSitePatterns | PS01206 | Amiloride-sensitive sodium channels signature. | 186 | 206 | - |
| 4 | g16464.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 312 | 334 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed