General information for g16538.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Enolase-phosphatase E1.
Parent gene
- The parent gene g16538 is located on chr_4: 9849401..9850448 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16538 | g16538.t1 | TSS | g16538.t1 | 9849313 | 9849313 |
| chr_4 | g16538 | g16538.t1 | isoform | g16538.t1 | 9849401 | 9850448 |
| chr_4 | g16538 | g16538.t1 | exon | g16538.t1.exon1 | 9849401 | 9849434 |
| chr_4 | g16538 | g16538.t1 | cds | g16538.t1.CDS1 | 9849401 | 9849434 |
| chr_4 | g16538 | g16538.t1 | exon | g16538.t1.exon2 | 9849757 | 9850448 |
| chr_4 | g16538 | g16538.t1 | cds | g16538.t1.CDS2 | 9849757 | 9850448 |
| chr_4 | g16538 | g16538.t1 | TTS | g16538.t1 | 9850462 | 9850462 |
Sequences
>g16538.t1 Gene=g16538 Length=726
ATGACAATTGAAGCTGTGATTTGTGATATTGAAGGAACAACAACATCAATTTCCTTCGTC
AAAGATGTTCTTTTTCCACTCGCATACAAAAATTGTGAAAAATTTCTCACTGAAAATTAT
AGGAACACCGAAATTCAACAAATTCTCCAAGACCTTCACGAACACTCAAAACTAGAAAAC
TCAGAAATACCAAATATTGAAAATGATAAAACTAAAGATATCAAAAATTTTACAACTTAC
ATCCAAACTCTCATTGATCAAGATAAAAAAGTCAAACCATTGAAACAACTTCAGGGAAAA
ATTTGGAAACTTGCATTTGAATCTGGTGAAATTAAAGGACATGTTTATGAAGATGTTTCA
AGAAATTTTCAAAAATGGACTGAAAAAGGAATCAAAATTTTTATTTATTCATCAGGATCT
GTTGAAGCTCAAAAGTTACTTTTTAGTCACTCACAGAGTGGGGATTTGACAAAATATTTG
AGTGGATATTTTGACACAACTGTAGGGCACAAACAAGAAGTTAAAAGTTATGAAAATATT
TTGGAAAAAATTAGATATGGAGCTGAAAAAGTTTTATTTTTGAGCGACATTTATGGTGAA
GTTAAAGCAGCTAAGCAAGTTGGAATTAACTGCAAAATATTGATCAGACCTGGAAATTAT
GAATTAAATGATGAAGAAAAAGAAGAATTTGAAACTGTCGAAAATTTTGATGAAATTTTT
GTATAA
>g16538.t1 Gene=g16538 Length=241
MTIEAVICDIEGTTTSISFVKDVLFPLAYKNCEKFLTENYRNTEIQQILQDLHEHSKLEN
SEIPNIENDKTKDIKNFTTYIQTLIDQDKKVKPLKQLQGKIWKLAFESGEIKGHVYEDVS
RNFQKWTEKGIKIFIYSSGSVEAQKLLFSHSQSGDLTKYLSGYFDTTVGHKQEVKSYENI
LEKIRYGAEKVLFLSDIYGEVKAAKQVGINCKILIRPGNYELNDEEKEEFETVENFDEIF
V
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g16538.t1 | CDD | cd01629 | HAD_EP | 5 | 218 | 0.0000000 |
| 6 | g16538.t1 | Gene3D | G3DSA:3.40.50.1000 | - | 6 | 230 | 0.0000000 |
| 7 | g16538.t1 | Gene3D | G3DSA:1.10.720.60 | - | 17 | 111 | 0.0000000 |
| 4 | g16538.t1 | Hamap | MF_01681 | Enolase-phosphatase E1 [mtnC]. | 2 | 239 | 35.4627760 |
| 2 | g16538.t1 | PANTHER | PTHR20371 | ENOLASE-PHOSPHATASE E1 | 3 | 239 | 0.0000000 |
| 3 | g16538.t1 | PANTHER | PTHR20371:SF1 | ENOLASE-PHOSPHATASE E1 | 3 | 239 | 0.0000000 |
| 1 | g16538.t1 | Pfam | PF13419 | Haloacid dehalogenase-like hydrolase | 65 | 211 | 0.0000300 |
| 11 | g16538.t1 | SFLD | SFLDF00044 | enolase-phosphatase | 1 | 239 | 0.0000000 |
| 12 | g16538.t1 | SFLD | SFLDG01129 | C1.5: HAD, Beta-PGM, Phosphatase Like | 1 | 239 | 0.0000000 |
| 5 | g16538.t1 | SUPERFAMILY | SSF56784 | HAD-like | 1 | 239 | 0.0000000 |
| 9 | g16538.t1 | TIGRFAM | TIGR01691 | enolase-ppase: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase | 3 | 239 | 0.0000000 |
| 10 | g16538.t1 | TIGRFAM | TIGR01549 | HAD-SF-IA-v1: HAD hydrolase, family IA, variant 1 | 92 | 208 | 0.0000006 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0000287 | magnesium ion binding | MF |
| GO:0016787 | hydrolase activity | MF |
| GO:0019509 | L-methionine salvage from methylthioadenosine | BP |
| GO:0043874 | acireductone synthase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K09880 (enolase-phosphatase E1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
