General information for g16586.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ubiquinone biosynthesis O-methyltransferase, mitochondrial.
Parent gene
- The parent gene g16586 is located on chr_4: 10101901..10103410 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16586 | g16586.t2 | TSS | g16586.t2 | 10101680 | 10101680 |
| chr_4 | g16586 | g16586.t2 | isoform | g16586.t2 | 10101681 | 10102570 |
| chr_4 | g16586 | g16586.t2 | exon | g16586.t2.exon1 | 10101681 | 10101729 |
| chr_4 | g16586 | g16586.t2 | exon | g16586.t2.exon2 | 10101807 | 10101927 |
| chr_4 | g16586 | g16586.t2 | cds | g16586.t2.CDS1 | 10101816 | 10101927 |
| chr_4 | g16586 | g16586.t2 | exon | g16586.t2.exon3 | 10102026 | 10102570 |
| chr_4 | g16586 | g16586.t2 | cds | g16586.t2.CDS2 | 10102026 | 10102570 |
| chr_4 | g16586 | g16586.t2 | TTS | g16586.t2 | 10102655 | 10102655 |
Sequences
>g16586.t2 Gene=g16586 Length=715
AGTTTTGCGTGAGTTTTGAGTGAATTAAAAATGGCTGATTTAAGATCAATTACATAAAAT
GATACCAGCTATAAGAATTCCATTGGTACTTGATGGTTTAACTGAAAGTGGTTTGATAAA
AAAAGAAAATCGAGATAAATCAAATGTTTTAGAAGGATTAAAAATTTTAGATGTTGGTTC
AGGAGCAGGTTTGATGTCAGAACCTCTTGCATTACTCGGAGCTGAAGTAGTCGGCATCGA
TCCAGCTGAAAAACTCGTCGAACGTGCAAGAGAACATTTGAAAATACATGAAAATCTTAA
AGTCACATACATTTGTGACACAATCGAAAGTTATTGTCGAGAAAACAAGGAAAAATTTGA
TGCTGTGACATTTTTCGATGTTGCCGAACATGTCGCTGATTTATCATCAGTTTTAAAGGC
AAGTGTCGAATGTCTTAAGCCAGGTGGTCAAATTTTCATCACAACATGGAATAAAACTTT
GATGGGTTGGCTTTATGGAATCATCATGCTTGAATATGTTGCTAGAATTGTTCCAAGAGG
TGCTCATTCGATTTCTATGTTTGTCAATCCTGAGGCCATCGAAGCATTGTTAAGAGTTTT
TGGATGTGAAGATATTGAGACTAGAGGAACTTGGTACAGTTTATGGACAAAAGATTGGAG
TTTCACTAACTACAAAGATGTTTTTTATGCTATGAAAGCTGTGAAGAGGAAGTAA
>g16586.t2 Gene=g16586 Length=218
MIPAIRIPLVLDGLTESGLIKKENRDKSNVLEGLKILDVGSGAGLMSEPLALLGAEVVGI
DPAEKLVERAREHLKIHENLKVTYICDTIESYCRENKEKFDAVTFFDVAEHVADLSSVLK
ASVECLKPGGQIFITTWNKTLMGWLYGIIMLEYVARIVPRGAHSISMFVNPEAIEALLRV
FGCEDIETRGTWYSLWTKDWSFTNYKDVFYAMKAVKRK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g16586.t2 | CDD | cd02440 | AdoMet_MTases | 35 | 137 | 0 |
| 5 | g16586.t2 | Gene3D | G3DSA:3.40.50.150 | Vaccinia Virus protein VP39 | 3 | 217 | 0 |
| 2 | g16586.t2 | PANTHER | PTHR43464 | METHYLTRANSFERASE | 28 | 216 | 0 |
| 3 | g16586.t2 | PANTHER | PTHR43464:SF25 | UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL | 28 | 216 | 0 |
| 1 | g16586.t2 | Pfam | PF13489 | Methyltransferase domain | 29 | 187 | 0 |
| 4 | g16586.t2 | SUPERFAMILY | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferases | 29 | 216 | 0 |
| 6 | g16586.t2 | TIGRFAM | TIGR01983 | UbiG: 3-demethylubiquinone-9 3-O-methyltransferase | 21 | 210 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008425 | 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity | MF |
| GO:0006744 | ubiquinone biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
