General information for g16591.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Trypsin-1.
Parent gene
- The parent gene g16591 is located on chr_4: 10138309..10139720 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16591 | g16591.t8 | isoform | g16591.t8 | 10137971 | 10139720 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon1 | 10137971 | 10138211 |
| chr_4 | g16591 | g16591.t8 | TTS | g16591.t8 | 10137978 | 10137978 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon2 | 10138330 | 10138374 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon3 | 10138434 | 10138463 |
| chr_4 | g16591 | g16591.t8 | cds | g16591.t8.CDS1 | 10138456 | 10138463 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon4 | 10138770 | 10138948 |
| chr_4 | g16591 | g16591.t8 | cds | g16591.t8.CDS2 | 10138770 | 10138948 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon5 | 10139068 | 10139195 |
| chr_4 | g16591 | g16591.t8 | cds | g16591.t8.CDS3 | 10139068 | 10139195 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon6 | 10139248 | 10139369 |
| chr_4 | g16591 | g16591.t8 | cds | g16591.t8.CDS4 | 10139248 | 10139369 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon7 | 10139434 | 10139589 |
| chr_4 | g16591 | g16591.t8 | cds | g16591.t8.CDS5 | 10139434 | 10139589 |
| chr_4 | g16591 | g16591.t8 | exon | g16591.t8.exon8 | 10139672 | 10139720 |
| chr_4 | g16591 | g16591.t8 | cds | g16591.t8.CDS6 | 10139672 | 10139720 |
| chr_4 | g16591 | g16591.t8 | TSS | g16591.t8 | 10139750 | 10139750 |
Sequences
>g16591.t8 Gene=g16591 Length=950
ATGTTGATTTTCAATTTCTTAATTCTTCTTTTTTTCTTCAATTTTGAAATTTTAAACGCA
AATGTTTTACGAAAGGGAAAAATTGTAGGCGGTTACTCAGCTGATTATTATCAATTTCCT
TATCAAGCGTCATTGCGTACTGCTTTAATGAGTACGCACTTTTGCGGGGCTTTTTTATTA
ACTGAACGTTGGCTCATGAGTGCAGGTCACTGCACTGATAAAAGAAAACCATCCGGTGTT
GTCGTCGTTGTTGGTGCATTTACTAGAGCAAAAGATGAAGGTGTCAAACATAATGTGCTA
CTTTTAGTCACACATCCTGAATTTGATGCAAAATCACTTCAAAATGACATTTCAATGATC
AAAACTGAAACTTTTATTGATTTTACAATCAGCATTCGACCAGTTCGATTGCAAGCAATG
CCAGCTGAAGAAGGAATAGAAGCTTTTGTATCTGGTTGGGGTTACATAAATTCAAAAGGA
ACAAATCCTGATGTTTTACAATGGATAAAAGTAAAAACAATTAATATTGATGAATGTCGT
GAAAAAATGAGTAAACAAAATGCTGAAAGAATTTTTGAAACAAATATTTGTGCTGATGCC
ATGGAAAGTACTGGATCTAATGGAAATGGTGGAGAATTTTAGGGGTGACAGTGGAGGGCC
TTTGGTGACAGTTGATGGAGCTTTAATAGGAATTGTTTCATGGGAAGTTCCATGCGGTAA
AGGTTTACCTGATGTTTACACTCAAGTTTCACCATTCAAAGATTGGATTGATGAAACAAT
TGATTTTCCTGAAATTGAAGAAGATGATGGCGATTATGAATATAATTTGATTGACATAAT
TCAAGATGATGCACATGATATGGAATTTATGGCAACTTAAATTTTGTGAACTTGATTTGA
TAAATAAAATTTTTTGATTATTTTCAATTGTTTCGAAAGAATTTAATTCA
>g16591.t8 Gene=g16591 Length=213
MLIFNFLILLFFFNFEILNANVLRKGKIVGGYSADYYQFPYQASLRTALMSTHFCGAFLL
TERWLMSAGHCTDKRKPSGVVVVVGAFTRAKDEGVKHNVLLLVTHPEFDAKSLQNDISMI
KTETFIDFTISIRPVRLQAMPAEEGIEAFVSGWGYINSKGTNPDVLQWIKVKTINIDECR
EKMSKQNAERIFETNICADAMESTGSNGNGGEF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g16591.t8 | CDD | cd00190 | Tryp_SPc | 28 | 202 | 6.40536E-51 |
| 6 | g16591.t8 | Gene3D | G3DSA:2.40.10.10 | - | 29 | 203 | 1.9E-38 |
| 7 | g16591.t8 | Gene3D | G3DSA:2.40.10.10 | - | 40 | 134 | 1.9E-38 |
| 2 | g16591.t8 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 21 | 203 | 7.9E-34 |
| 3 | g16591.t8 | PANTHER | PTHR24276:SF89 | IP07603P | 21 | 203 | 7.9E-34 |
| 1 | g16591.t8 | Pfam | PF00089 | Trypsin | 28 | 204 | 5.4E-33 |
| 9 | g16591.t8 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 10 | g16591.t8 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 11 | g16591.t8 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 13 | - |
| 12 | g16591.t8 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 20 | - |
| 8 | g16591.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 213 | - |
| 14 | g16591.t8 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 66 | 71 | - |
| 16 | g16591.t8 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 28 | 213 | 19.368 |
| 15 | g16591.t8 | SMART | SM00020 | trypsin_2 | 27 | 210 | 1.8E-22 |
| 4 | g16591.t8 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 21 | 203 | 1.85E-42 |
| 5 | g16591.t8 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed