General information for g16732.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 305a1.
Parent gene
- The parent gene g16732 is located on chr_4: 10733251..10734189 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16732 | g16732.t3 | TTS | g16732.t3 | 10731461 | 10731461 |
| chr_4 | g16732 | g16732.t3 | isoform | g16732.t3 | 10732434 | 10733555 |
| chr_4 | g16732 | g16732.t3 | exon | g16732.t3.exon1 | 10732434 | 10733251 |
| chr_4 | g16732 | g16732.t3 | cds | g16732.t3.CDS1 | 10732435 | 10733235 |
| chr_4 | g16732 | g16732.t3 | exon | g16732.t3.exon2 | 10733413 | 10733555 |
| chr_4 | g16732 | g16732.t3 | TSS | g16732.t3 | 10734423 | 10734423 |
Sequences
>g16732.t3 Gene=g16732 Length=961
GGTCAGCATAAGGCCTTTGATTACTTATCAAAAAAATACAACTCACCAATTTTCAGTATA
AAACTTGGAAGAGAACTTGTAGTTGTTGTTAGAAGTTATGAATTAATAAAAGAAGTTCAT
TCAAGAGAAGAATTTGATGGAAGACCGGATAATTTCTTTATGCGTCTTCGTACGATGGGC
ACACGTTTAGGCATCACTTGCACTGATGGTCGACTTTGGCAGGAACAAAGAAGTTTTGTT
GTTCGTCAACTTCGAAATGTTGGCTATGGAAAGTCAAAAATGGAAGAGCAAATTCATGTA
GAACTGAATGAAATTTTAGAAATTATCAGAAGTAGCAAAGACACTCCGATTTGGCCAGGT
GAAACAAACTTTATAGCAACAAGTGTGATAAATATTCTTTGGACTTTTACAACTGGTGCA
AAAATCAAACGTAATGATGCAAGACTAATAAAATTTTTGAATTTACTTCAAAAACGATCA
AAAGCATTTGAAATTAGTGGTGGATATTTAAATCAAATGCCATGGTTGAGATTTTTTGCG
CCAGAAGCGACTGGTTATAATTTGGTTAAAAATCTTAATTCGGAATTTTACAAATTCTTT
ATGGAAGTCATCAATGAGCATTTAATGACATATAGTGAGGATAAATCAAATGAAGATCTC
ATTTATGCTTACATCAAAGAAATGAATGAACAAAAAGACAAAGAAAATTCAACATTCAAA
ATTGATCAACTTGTGATGGTCATTTTAGACATTTTTATAGCTGGTGCAAGTTCAACTGGA
ATAACAATCGATTTAGCTTTGATGTCAATGTTGATTTATCCAGAAATTCAAATGAAAGTT
CAGAAAGAAATTGATGAAATTCTCAATGAAGACAGTGAAATGTTGAAATATTTTGATAAA
AAGAAAATGCCATTTACAGAAGCAGTATTGCTTGAGGTTCAAAGATTTTATAGTATTGTT
C
>g16732.t3 Gene=g16732 Length=267
MRLRTMGTRLGITCTDGRLWQEQRSFVVRQLRNVGYGKSKMEEQIHVELNEILEIIRSSK
DTPIWPGETNFIATSVINILWTFTTGAKIKRNDARLIKFLNLLQKRSKAFEISGGYLNQM
PWLRFFAPEATGYNLVKNLNSEFYKFFMEVINEHLMTYSEDKSNEDLIYAYIKEMNEQKD
KENSTFKIDQLVMVILDIFIAGASSTGITIDLALMSMLIYPEIQMKVQKEIDEILNEDSE
MLKYFDKKKMPFTEAVLLEVQRFYSIV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g16732.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 267 | 7.1E-55 |
| 2 | g16732.t3 | PANTHER | PTHR24303:SF13 | CYTOCHROME P450 305A1-RELATED | 1 | 267 | 1.3E-100 |
| 3 | g16732.t3 | PANTHER | PTHR24303 | HEME-BINDING MONOOXYGENASE FAMILY | 1 | 267 | 1.3E-100 |
| 6 | g16732.t3 | PRINTS | PR00463 | E-class P450 group I signature | 75 | 93 | 4.3E-17 |
| 7 | g16732.t3 | PRINTS | PR00463 | E-class P450 group I signature | 190 | 207 | 4.3E-17 |
| 5 | g16732.t3 | PRINTS | PR00463 | E-class P450 group I signature | 210 | 236 | 4.3E-17 |
| 4 | g16732.t3 | PRINTS | PR00463 | E-class P450 group I signature | 254 | 267 | 4.3E-17 |
| 1 | g16732.t3 | Pfam | PF00067 | Cytochrome P450 | 9 | 267 | 4.9E-35 |
| 10 | g16732.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 190 | - |
| 12 | g16732.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 191 | 220 | - |
| 11 | g16732.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 221 | 267 | - |
| 8 | g16732.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 7 | 267 | 1.7E-40 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
