General information for g16832.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Guanylate cyclase 32E.
Parent gene
- The parent gene g16832 is located on chr_4: 11302323..11308817 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16832 | g16832.t1 | isoform | g16832.t1 | 11302323 | 11308817 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon1 | 11302323 | 11302543 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS1 | 11302323 | 11302543 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon2 | 11302607 | 11302772 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS2 | 11302607 | 11302772 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon3 | 11302824 | 11303043 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS3 | 11302824 | 11303043 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon4 | 11303101 | 11303897 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS4 | 11303101 | 11303897 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon5 | 11304247 | 11304481 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS5 | 11304247 | 11304481 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon6 | 11304533 | 11304828 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS6 | 11304533 | 11304828 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon7 | 11305127 | 11305450 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS7 | 11305127 | 11305450 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon8 | 11306670 | 11307004 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS8 | 11306670 | 11307004 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon9 | 11307210 | 11307362 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS9 | 11307210 | 11307362 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon10 | 11307421 | 11307538 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS10 | 11307421 | 11307538 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon11 | 11307866 | 11308000 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS11 | 11307866 | 11308000 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon12 | 11308059 | 11308170 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS12 | 11308059 | 11308170 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon13 | 11308264 | 11308388 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS13 | 11308264 | 11308388 |
| chr_4 | g16832 | g16832.t1 | exon | g16832.t1.exon14 | 11308683 | 11308817 |
| chr_4 | g16832 | g16832.t1 | cds | g16832.t1.CDS14 | 11308683 | 11308817 |
| chr_4 | g16832 | g16832.t1 | TSS | g16832.t1 | NA | NA |
| chr_4 | g16832 | g16832.t1 | TTS | g16832.t1 | NA | NA |
Sequences
>g16832.t1 Gene=g16832 Length=3372
ATGAAAGCACTTACAATTGTTTTAGTGATGTTGAGTTTGTTGACATTGTGTAATGGATTT
GAAGTATGTGGAAATTATAAGAAAACAGACAAGACAATAAGAATTGGATTTTTATCAAGA
TATACAACATCTAAAGTAATTTTAGGTGCTGTACCTTTGGCAATTGAACACATTAATTCA
AACAATAAACTTCTTCCTGGAAGGGTACTCGAATATCAAGCTGTAAATATTGTCAAAACA
TCTTCAGTTGAAATTATCAAGAACATGACAAATTTAATGTTAAATTCAATTGACGCATTT
ATTGGACCGGAAGATTTGTGCTTTAATGAAGCACTTGTTGCTTCTGCTTGGAACATTCCA
TTAATTTCATATTCTTGTGCTGATATGAAAGTTGGAAATAAAACACAATTTTCTACGTTC
GCTAGGTCATCTCTCGGTCCACCTGAAAAAATATCGAAAAGTGTTATTGCATTGCTGTCA
TATTATAATTGGCGGAAGTTCATTATTGATTTATCATCGAAAAACAACCTAAATTTGACT
CAAATAAAAGAATTTAGTGACTATTTGAGCAACAAACATGAAAATATGGAAAAAATTGTT
GATGAAACCTTTAAAAAGACACGAATTTATGTATTTGTTGGTGGACAATATGCTTTTGTC
GCTTTTATGCATTGCCTTGAAGAACGTGATTTATTAAATAGCGGTGAATATATGGTAATT
TTAGTAAATGATGAATTCACATTTCCCAACAACGATATAAACCAATCAGATCATCACCTT
TATCGTTTCTTTTCACGCTCTAAGAAAAAAACCTTAAACCCAAAAACTTTTCAATCAGTA
TTGCAAATAACCTTAACAACACCATCATCAAATTTAACACAATTTCATAAAGAAATTCGA
GCAAAAGCTCATGAAAAGTTTAATGTTCCGCTTCATTCGAGAATTTTACCACTGTTTTTG
TCAGCCAAAGCTGCTTATCTTTATGATGCAGTGATGATTTATGCAAAAGCCGCGACACAA
ATGCTGAATAGTGGTGGAAATATTAAAGACGGAACGACTTTGATGCAGAAATTTGTTTTT
AATAATACTTTTACAAGTGTACAAGGATTTGATTGGCTTCAAATTGGAAAATCGATTAAC
AATAATCCATCATCGAGTCTCGATTTACTGAAGGAAACACTTTTGGAAGTAAAGGATTAT
ATCGACGAGAGTGAAAATATTCTGATTCTTGGCGATTTTAATCACGAATTGAAATGGGGC
AGTCCACTCGAGTTGTTCATGCTTCAATCTTTTGGCACAAGACTGTTCTCACCACGCGAA
TTGACAACAAATGCTCGAACAGTTATCAATGGAGTGTTTGGACGAATCGAAGATTATAAT
GTTGAAGTTTTTATCTATGAATCGTATGCATCTCATCACAAACCATTAGTCATCCGAATT
TTCATTGATGAAAATGGAAATGCTGAAGGAAATTTCTCACTTTTCACAATCAGTTATATC
AATAAAACATATTCAGTTCAACAAGTTGGTACATTTGTGGAATCAAATTCACAAAATGGA
ATTCCTAAATTTCAACTTAATAACAAATCTCAAATTTCATGGGTAAATGGAAATCCGCCT
ATCGATGAACCATCAGAATGTGGTTTTGAAGGTTGTTCATACAATTGGAGTTATGCAATC
ATGAGTTGCTTTACAATTCTTGGTTTTATGTCATTTATTGGAATTATCATAAAACATTAT
CGCTATGAACAACGACTTCAATCATTACTTTGGAAAATTGATTTGAAAGAAATAACACAG
CTAGCAGTAGAAGATGAAACTTATGGTTACAATAAAATTATGAAAGAAGGAAATAAGGAA
CTCATCATTGCAAAATATCGAGGCAATTTTGTTGCCATGAAGCGAATCAATAAACGAGCA
ATTGAATTAACAAGAAATGTAAGAAAAGAACTGCAAATTATGCGTGAGCTTCGTCATGAA
AACCTCTCTCAATTTATTGGAGTGACACTTGAAACATCAAATATTTTTATTATAAGTCAA
TTTTATGCTAGAGGAAGTCTTGAAAATCTTTTGACAAATGACGATCTAAAGCTTGATGAA
ATGTTCATCTCATCTCTGGTGTTTGATGCAATTAAAGGAATGATTTTCCTTCATGATAGT
GAAATTGGTTTCCATGGAAATTTAAGAACAAGTAATTGCCTTGTTGACTCACGATGGTGT
TTAAAAATTGCTGATTTTGGTTTAAATGAATTTCAAAATGGTGCAACAAATCGCTATAAA
TTGAAAAATTTATTAACAAAAGCACCAGAATTATTTGACCCAGATGCACCAATTGGAACT
CAAAAAAGTGATGTTTATTCTTTTGGAATTCTTCTTTATGAAATTTATGGTCGAAAAGGT
CCATTTGGAATTGGTTATGAATTCAATGAATCAACAATTCCAATTTATGAAGACATTTTG
GCAAAATTAAGAAATCCACAAAAACACATTAGACCATCAATGAATAATTTTCGTGCACCT
GATTGTGTAAAAGAAACAATAACATTATGTTGGCATAAAGATCCATTTGAACGTCCTGAT
ATGAAAATGATTAAGTTAAAATTAAAAGAACTTAAATGGGGATTAAAATCAAATATTTTT
GATAACATCATGTTATTGATGGAAAAATATGCAAGTAATTTAGAAGAATTGGTCCAAGAA
CGAACAAATCAATTGGTAGAAGAAAAGAAAAAAACGGAAGGTTTACTTTTAAGAATGCTA
CCAAAATCAGTAGCAGAAACTCTAATCTGTGGTCATCAATTAAATGCCGAATATTATGAC
TGTGTAACAATAATGTTCAGTGATTTAGTCGGATTTACAGAAATCTGCTCAACAAGTACA
CCACATGAAGTTGTAGAAATGTTGAATTTTCTTTACTCATTGATGGACTCAATTATTACA
AACTTTGATTGTTATAAAATTGAAACAATTGGTGATGCTTACATGGTCTGCTCAGGAGCA
CCAATAAGAAATGAAGACCATGCATTGCAAGTTGCGTCATTGGCATTAAATATTATGGAA
AAAGTGTCAACTTTGGAAATCCCACATAAGAAGGGTGAATTTTTTAAGATTAGAATGGGA
CTTCATTCAGGTCAAGTCGTAGCTGGAATTGTTGGTGATAAAATGCCACGTTATTGTTTA
TTTGGTGATAGTGTCAATACAGCAAGTCGTATGGAAAGCACTTCAGAACCACTTAAAATC
CAAATATCATTTGACACTTTCAAACTACTTAAAAAGTATGGAAACTTTGTTTGCATTGAA
CGTGATGGTGGAGTAACATTTATCAAAGGAAAAGGACAAATGAAGACATTCTGGTTGATG
GGAAAGCATTGA
>g16832.t1 Gene=g16832 Length=1123
MKALTIVLVMLSLLTLCNGFEVCGNYKKTDKTIRIGFLSRYTTSKVILGAVPLAIEHINS
NNKLLPGRVLEYQAVNIVKTSSVEIIKNMTNLMLNSIDAFIGPEDLCFNEALVASAWNIP
LISYSCADMKVGNKTQFSTFARSSLGPPEKISKSVIALLSYYNWRKFIIDLSSKNNLNLT
QIKEFSDYLSNKHENMEKIVDETFKKTRIYVFVGGQYAFVAFMHCLEERDLLNSGEYMVI
LVNDEFTFPNNDINQSDHHLYRFFSRSKKKTLNPKTFQSVLQITLTTPSSNLTQFHKEIR
AKAHEKFNVPLHSRILPLFLSAKAAYLYDAVMIYAKAATQMLNSGGNIKDGTTLMQKFVF
NNTFTSVQGFDWLQIGKSINNNPSSSLDLLKETLLEVKDYIDESENILILGDFNHELKWG
SPLELFMLQSFGTRLFSPRELTTNARTVINGVFGRIEDYNVEVFIYESYASHHKPLVIRI
FIDENGNAEGNFSLFTISYINKTYSVQQVGTFVESNSQNGIPKFQLNNKSQISWVNGNPP
IDEPSECGFEGCSYNWSYAIMSCFTILGFMSFIGIIIKHYRYEQRLQSLLWKIDLKEITQ
LAVEDETYGYNKIMKEGNKELIIAKYRGNFVAMKRINKRAIELTRNVRKELQIMRELRHE
NLSQFIGVTLETSNIFIISQFYARGSLENLLTNDDLKLDEMFISSLVFDAIKGMIFLHDS
EIGFHGNLRTSNCLVDSRWCLKIADFGLNEFQNGATNRYKLKNLLTKAPELFDPDAPIGT
QKSDVYSFGILLYEIYGRKGPFGIGYEFNESTIPIYEDILAKLRNPQKHIRPSMNNFRAP
DCVKETITLCWHKDPFERPDMKMIKLKLKELKWGLKSNIFDNIMLLMEKYASNLEELVQE
RTNQLVEEKKKTEGLLLRMLPKSVAETLICGHQLNAEYYDCVTIMFSDLVGFTEICSTST
PHEVVEMLNFLYSLMDSIITNFDCYKIETIGDAYMVCSGAPIRNEDHALQVASLALNIME
KVSTLEIPHKKGEFFKIRMGLHSGQVVAGIVGDKMPRYCLFGDSVNTASRMESTSEPLKI
QISFDTFKLLKKYGNFVCIERDGGVTFIKGKGQMKTFWLMGKH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 29 | g16832.t1 | CDD | cd07302 | CHD | 942 | 1119 | 6.25472E-65 |
| 21 | g16832.t1 | Coils | Coil | Coil | 887 | 911 | - |
| 18 | g16832.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 47 | 370 | 1.1E-52 |
| 20 | g16832.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 150 | 291 | 1.1E-52 |
| 17 | g16832.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 479 | 489 | 2.0E-7 |
| 19 | g16832.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 490 | 549 | 2.0E-7 |
| 16 | g16832.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 614 | 907 | 7.2E-51 |
| 15 | g16832.t1 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 929 | 1115 | 7.5E-71 |
| 5 | g16832.t1 | PANTHER | PTHR11920:SF475 | GUANYLATE CYCLASE | 33 | 369 | 8.5E-244 |
| 8 | g16832.t1 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 33 | 369 | 8.5E-244 |
| 6 | g16832.t1 | PANTHER | PTHR11920:SF475 | GUANYLATE CYCLASE | 478 | 609 | 8.5E-244 |
| 9 | g16832.t1 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 478 | 609 | 8.5E-244 |
| 4 | g16832.t1 | PANTHER | PTHR11920:SF475 | GUANYLATE CYCLASE | 623 | 1122 | 8.5E-244 |
| 7 | g16832.t1 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 623 | 1122 | 8.5E-244 |
| 2 | g16832.t1 | Pfam | PF01094 | Receptor family ligand binding region | 48 | 355 | 1.0E-26 |
| 3 | g16832.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 630 | 867 | 1.5E-34 |
| 1 | g16832.t1 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 935 | 1120 | 4.2E-60 |
| 24 | g16832.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 25 | g16832.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 26 | g16832.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 28 | g16832.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 23 | g16832.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 555 | - |
| 27 | g16832.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 556 | 577 | - |
| 22 | g16832.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 578 | 1123 | - |
| 32 | g16832.t1 | ProSitePatterns | PS00452 | Guanylate cyclase signature. | 1049 | 1072 | - |
| 34 | g16832.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 608 | 871 | 28.273 |
| 33 | g16832.t1 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 943 | 1072 | 47.105 |
| 31 | g16832.t1 | SMART | SM00220 | serkin_6 | 611 | 872 | 3.9E-14 |
| 30 | g16832.t1 | SMART | SM00044 | cyc_6 | 907 | 1100 | 2.2E-94 |
| 11 | g16832.t1 | SUPERFAMILY | SSF53822 | Periplasmic binding protein-like I | 31 | 377 | 5.65E-44 |
| 10 | g16832.t1 | SUPERFAMILY | SSF53822 | Periplasmic binding protein-like I | 480 | 549 | 5.96E-6 |
| 12 | g16832.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 625 | 909 | 3.61E-46 |
| 13 | g16832.t1 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 931 | 1119 | 2.04E-60 |
| 14 | g16832.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016849 | phosphorus-oxygen lyase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
- This transcript has been annotated with K01769 (guanylate cyclase, other)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed