General information for g17015.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Bifunctional glutamate/proline–tRNA ligase.
Parent gene
- The parent gene g17015 is located on chr_4: 12162767..12163690 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17015 | g17015.t7 | TSS | g17015.t7 | 12156272 | 12156272 |
| chr_4 | g17015 | g17015.t7 | isoform | g17015.t7 | 12156363 | 12160085 |
| chr_4 | g17015 | g17015.t7 | exon | g17015.t7.exon1 | 12156363 | 12157603 |
| chr_4 | g17015 | g17015.t7 | cds | g17015.t7.CDS1 | 12156363 | 12157603 |
| chr_4 | g17015 | g17015.t7 | exon | g17015.t7.exon2 | 12157660 | 12157922 |
| chr_4 | g17015 | g17015.t7 | cds | g17015.t7.CDS2 | 12157660 | 12157922 |
| chr_4 | g17015 | g17015.t7 | exon | g17015.t7.exon3 | 12160076 | 12160085 |
| chr_4 | g17015 | g17015.t7 | cds | g17015.t7.CDS3 | 12160076 | 12160083 |
| chr_4 | g17015 | g17015.t7 | TTS | g17015.t7 | 12160643 | 12160643 |
Sequences
>g17015.t7 Gene=g17015 Length=1514
ATGCCAATCAAAGTAATTTGTGATTTAAAGAATCCACCAATTGGTAAGTTACAGATTAAT
CATAAAATTACTTCTGCAGAAAGCTTAAAATTCAAAATTTTAAATTTTCATGAAATATTT
TTAATTTTTTATGAAATCAAATTTGCAGGTGCTTTGGTTGTTGCCGAAATTATTAAATCA
ACTGATCCAGTTGTAATTGAATGGAGCGATAAAGAAGCATCTATCAATTTTGGTAACAAT
TCACTTTCATGTGTGAGCAACAACGACCTTTTAAGAGCTCTAGCTCGACAAGCCGGAAGT
TATCAGCTTTATGGAAAAACTCCGATTGAAAGAACTCAAATTGATCATTGGTTGACTTAC
TGCCTCAATTATGATGCTCATATTGATGAAGCAATTCAATATTTGAATAAATGTTTAGCT
CCATTGACTTATTTAGTGTCAAATAAGCTGACAATTGCTGATTTAGCTGTGTTCAATGAA
TTTTTTGTCAAGTATGATCTTTTGAAGAAATCTGGTTTGCCTAGTCATGTTCAACGCTGG
TATAACCTCATCAGTTCATTGCCAACAGTTCAAACTGTGCTTTCATCACTTCCAAAAGAT
GGTAAATCTACAAAATCAAAGAAACAAGAAAAACAAGAATTTTCAGCAGCTCTTGGTGTT
GGCGATAGAAAGCAAGAAGGAAAATTTGTCGATCTTCCAGGAGCTGAAATGGGAAAAGTT
GTTGTTCGCTTTCCTCCTGAAGCATCAGGGTATTTGCACATTGGTCATGCTAAAGCAGCT
CTTTTAAATCAATATTATCAGCAAGCATTTGATGGAAAATTGATTATGAGATTTGATGAC
ACCAATCCAGCTAAAGAAAATGTTCATTTTGAGGAAGTCATTTTGGAAGATGTAGAAATG
CTTCAAATTAAACCTGATGCATTCACTTACACCTCAGATTATTTTGATATCATGCTTAAA
TATTGTGAGCAATTAATGAAAGAAGGAAAAGCATATGCTGATGATACTGATCCTGAATTG
ATGAAACAAGAACGTGAACAGAAAGTTGAGTCAAAGAACCGAAATAATTCGGTTGAAAAG
AATTTTGAAATGTGGAAAGAAATGTTGGCTGGCACAGAAAAAGGACAAAAATGTTGTATT
CGAGCAAAAATTGACATGTCATCACCAAATGGATGCCTTCGTGATCCAACAATTTATCGT
TGCAAAAATGAACCACATCCACGTACTGGAACTAATTACAAAGTTTATCCAACTTATGAT
TTTGCTTGTCCTATTGTTGATGCAATTGAAAATGTGACTCATACTCTTCGTACAATGGAA
TATCACGATCGTGATGATCAATTTTATTGGTTTATTGATGCATTGAAACTTCGTAAACCA
TACATTTGGGAATATTCACGTTTAAATATGACTAACACTGTTCTATCTAAACGTAAATTG
ACTTGGTTTGTTGAAAACGGTCTGGTTGATGGCTGGGATGATCCTCGTTTTCCTACAGTT
CGAGGTGTTTTACG
>g17015.t7 Gene=g17015 Length=504
MPIKVICDLKNPPIGKLQINHKITSAESLKFKILNFHEIFLIFYEIKFAGALVVAEIIKS
TDPVVIEWSDKEASINFGNNSLSCVSNNDLLRALARQAGSYQLYGKTPIERTQIDHWLTY
CLNYDAHIDEAIQYLNKCLAPLTYLVSNKLTIADLAVFNEFFVKYDLLKKSGLPSHVQRW
YNLISSLPTVQTVLSSLPKDGKSTKSKKQEKQEFSAALGVGDRKQEGKFVDLPGAEMGKV
VVRFPPEASGYLHIGHAKAALLNQYYQQAFDGKLIMRFDDTNPAKENVHFEEVILEDVEM
LQIKPDAFTYTSDYFDIMLKYCEQLMKEGKAYADDTDPELMKQEREQKVESKNRNNSVEK
NFEMWKEMLAGTEKGQKCCIRAKIDMSSPNGCLRDPTIYRCKNEPHPRTGTNYKVYPTYD
FACPIVDAIENVTHTLRTMEYHDRDDQFYWFIDALKLRKPYIWEYSRLNMTNTVLSKRKL
TWFVENGLVDGWDDPRFPTVRGVL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g17015.t7 | Coils | Coil | Coil | 341 | 361 | - |
| 13 | g17015.t7 | Gene3D | G3DSA:1.20.1050.130 | - | 46 | 205 | 2.5E-36 |
| 12 | g17015.t7 | Gene3D | G3DSA:3.40.50.620 | HUPs | 210 | 504 | 2.6E-115 |
| 3 | g17015.t7 | PANTHER | PTHR43382 | PROLYL-TRNA SYNTHETASE | 51 | 504 | 1.8E-221 |
| 5 | g17015.t7 | PRINTS | PR00987 | Glutamyl-tRNA synthetase signature | 243 | 255 | 1.6E-17 |
| 8 | g17015.t7 | PRINTS | PR00987 | Glutamyl-tRNA synthetase signature | 257 | 268 | 1.6E-17 |
| 4 | g17015.t7 | PRINTS | PR00987 | Glutamyl-tRNA synthetase signature | 272 | 285 | 1.6E-17 |
| 7 | g17015.t7 | PRINTS | PR00987 | Glutamyl-tRNA synthetase signature | 417 | 427 | 1.6E-17 |
| 6 | g17015.t7 | PRINTS | PR00987 | Glutamyl-tRNA synthetase signature | 433 | 441 | 1.6E-17 |
| 2 | g17015.t7 | Pfam | PF14497 | Glutathione S-transferase, C-terminal domain | 126 | 196 | 1.0E-4 |
| 1 | g17015.t7 | Pfam | PF00749 | tRNA synthetases class I (E and Q), catalytic domain | 239 | 503 | 4.0E-107 |
| 11 | g17015.t7 | ProSitePatterns | PS00178 | Aminoacyl-transfer RNA synthetases class-I signature. | 246 | 257 | - |
| 10 | g17015.t7 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 89 | 192 | 2.09E-16 |
| 9 | g17015.t7 | SUPERFAMILY | SSF52374 | Nucleotidylyl transferase | 239 | 491 | 6.97E-76 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0006418 | tRNA aminoacylation for protein translation | BP |
| GO:0000166 | nucleotide binding | MF |
| GO:0043039 | tRNA aminoacylation | BP |
| GO:0004812 | aminoacyl-tRNA ligase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed