General information for g17053.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamine-dependent NAD(+) synthetase.
Parent gene
- The parent gene g17053 is located on chr_4: 12322973..12325635 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17053 | g17053.t4 | isoform | g17053.t4 | 12321475 | 12322973 |
| chr_4 | g17053 | g17053.t4 | exon | g17053.t4.exon1 | 12321475 | 12322589 |
| chr_4 | g17053 | g17053.t4 | cds | g17053.t4.CDS1 | 12321475 | 12322589 |
| chr_4 | g17053 | g17053.t4 | TTS | g17053.t4 | 12321495 | 12321495 |
| chr_4 | g17053 | g17053.t4 | exon | g17053.t4.exon2 | 12322654 | 12322973 |
| chr_4 | g17053 | g17053.t4 | cds | g17053.t4.CDS2 | 12322654 | 12322849 |
| chr_4 | g17053 | g17053.t4 | TSS | g17053.t4 | NA | NA |
Sequences
>g17053.t4 Gene=g17053 Length=1435
GTGGGGCGGAAATTCAGCAATTGGATTAAATGGTGAAATAATTGCAAAAAGCAAGCAATT
TTCATTGTTGGATGTTGAAGTTACAGTAGCAACTATTGATTTAGAAGACATCAGAAGTTA
TCGAATGGCTTTAAGATCACGTTGCACTTTTGGAGCTTCTAGTCCAGTTTATCCAAGAAT
TGCTGTCGATTGTGAACTTTCAAGTCGTCGCGATGTTTTGATTCCTGTGAATTTACCATT
AGAATGGATTAATCATACTCCAGAAGAAGAAATTGCTATGGGTCCTGCATGCTGGTTGTG
GGATTATCTTCGTCGTTCAGGTCAAGGTGGTTATTTTTTGCCATTGTCAGGCGGTATTGA
TTCTTGCAGTACAGCATGCATTGTGTATAACATGTGTCAAATGGTAGTTGATGCAATTCA
AAATGGCGAAGATGACGTTTTACGTGATGTAAGAAAAATCACAGCTGATCCAGAATTTTC
ACCTCAAACAGCAGCTGAATTGTGCAATAAAATATTTGTGACTTGTTTTATGGGATCAAA
AAATTCTAGTAAAGAAACAAAAAATCGTGCAGCAGTTTTAGCAAATGAAATTGGAAGTTA
TCATTTGGAACTCAATATTGACAGTGTTTTTGACACAATTTTATCAGTTTTCAAAGCAGT
CACTGGTTTTATACCAAAATATCGGGTTCAAGGTGGTTCCACAAGAGAAAATCTTGCATT
GCAAAACATTCAAGCAAGAGTTCGAATGGTGCTGTCTTATCTTTTTGCACAATTAGTTTT
ATGGACAAGAAATCGTCCAGGTGGTCTTTTAGTCCTTGGTTCTGCAAATGTTGATGAATC
ATTGCGTGGTTATATGACAAAATATGACTGCTCTTCAGCTGACGTAAATCCAATTGGTGG
AATTTCAAAAACTGACTTGAGAAGATTTTTAAATTATGCCATGAAAAAATATAATATACC
AATTTTAAATGAAATATTTTTGGCTCAACCAACAGCTGAACTTGAGCCACTTCATGAAGG
ACAATTAGCACAAACAGATGAGCAAGACATGGGAATGACTTATGCTGAATTGAGCGAATT
TGGAAGATTGAGAAAACAAGCAGCATGTGGTCCATATTCTATGTTTTGTAAATTAGTGGC
AACATGGCGTAATGAATTTTCTCCAAAGGAAGTAAGTGACAAAGTTAAACATTTCTTCCG
TTGTTATGCAATAAATCGTCATAAAATGACTGTTTTAACTCCTTCTGTACATATGGAAAG
ATATTCACCTGATGATAATCGATTTGATCATCGACCTTTCCTTTATCGAACTAATTGGAG
TTGGCAATTTAAAGCCATTGATAATGAGTTGGAAAGAATTATGCAAACTTCCACTAAAGG
AGCACAAAAACATGACAGAAATGAACATGAAAATAAAGAAAAAGAAGAAATTTAA
>g17053.t4 Gene=g17053 Length=436
MALRSRCTFGASSPVYPRIAVDCELSSRRDVLIPVNLPLEWINHTPEEEIAMGPACWLWD
YLRRSGQGGYFLPLSGGIDSCSTACIVYNMCQMVVDAIQNGEDDVLRDVRKITADPEFSP
QTAAELCNKIFVTCFMGSKNSSKETKNRAAVLANEIGSYHLELNIDSVFDTILSVFKAVT
GFIPKYRVQGGSTRENLALQNIQARVRMVLSYLFAQLVLWTRNRPGGLLVLGSANVDESL
RGYMTKYDCSSADVNPIGGISKTDLRRFLNYAMKKYNIPILNEIFLAQPTAELEPLHEGQ
LAQTDEQDMGMTYAELSEFGRLRKQAACGPYSMFCKLVATWRNEFSPKEVSDKVKHFFRC
YAINRHKMTVLTPSVHMERYSPDDNRFDHRPFLYRTNWSWQFKAIDNELERIMQTSTKGA
QKHDRNEHENKEKEEI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g17053.t4 | CDD | cd00553 | NAD_synthase | 46 | 368 | 6.40462E-92 |
| 5 | g17053.t4 | Gene3D | G3DSA:3.40.50.620 | HUPs | 46 | 414 | 4.7E-139 |
| 9 | g17053.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 415 | 436 | - |
| 8 | g17053.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 417 | 436 | - |
| 2 | g17053.t4 | PANTHER | PTHR23090:SF9 | GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE | 11 | 410 | 2.7E-186 |
| 3 | g17053.t4 | PANTHER | PTHR23090 | NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE | 11 | 410 | 2.7E-186 |
| 6 | g17053.t4 | PIRSF | PIRSF006630 | NADS_GAT | 25 | 402 | 3.0E-86 |
| 1 | g17053.t4 | Pfam | PF02540 | NAD synthase | 57 | 319 | 1.3E-26 |
| 4 | g17053.t4 | SUPERFAMILY | SSF52402 | Adenine nucleotide alpha hydrolases-like | 39 | 380 | 1.13E-62 |
| 10 | g17053.t4 | TIGRFAM | TIGR00552 | nadE: NAD+ synthetase | 48 | 369 | 1.2E-24 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004359 | glutaminase activity | MF |
| GO:0003952 | NAD+ synthase (glutamine-hydrolyzing) activity | MF |
| GO:0005737 | cytoplasm | CC |
| GO:0009435 | NAD biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01950 (NAD+ synthase (glutamine-hydrolysing))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
