General information for g17053.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamine-dependent NAD(+) synthetase.
Parent gene
- The parent gene g17053 is located on chr_4: 12322973..12325635 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17053 | g17053.t7 | TTS | g17053.t7 | 12321495 | 12321495 |
| chr_4 | g17053 | g17053.t7 | isoform | g17053.t7 | 12322175 | 12323816 |
| chr_4 | g17053 | g17053.t7 | exon | g17053.t7.exon1 | 12322175 | 12322589 |
| chr_4 | g17053 | g17053.t7 | cds | g17053.t7.CDS1 | 12322177 | 12322589 |
| chr_4 | g17053 | g17053.t7 | exon | g17053.t7.exon2 | 12322654 | 12323334 |
| chr_4 | g17053 | g17053.t7 | cds | g17053.t7.CDS2 | 12322654 | 12323334 |
| chr_4 | g17053 | g17053.t7 | exon | g17053.t7.exon3 | 12323403 | 12323816 |
| chr_4 | g17053 | g17053.t7 | cds | g17053.t7.CDS3 | 12323403 | 12323544 |
| chr_4 | g17053 | g17053.t7 | TSS | g17053.t7 | NA | NA |
Sequences
>g17053.t7 Gene=g17053 Length=1510
ATGGGTCGAAAAGTAACAGTTGCAGTATCTACATTGAGTCAATGGGCTTTGGATTTTGAA
GGCAACATGGCAAGAATTATACAATCAATTTTGGAAGCACGTGAACTTGGAGCTCTTTAT
CGTACTGGACCAGAATTGGAAATTACGTAAGAACTTTTAAAAATAGAATTTTTTGATAAA
CTTTTAAATTTTTTTAAAGTGGTTACAGTTGTGAAGATCATTTTTATGAGCCAGATACAT
TCTTGCATTCATGGGAAGTTCTTTTGCAAATTATGATGTCACCAGTTTGTGAAAATATGC
TGATTGATGTTGGAATGCCAGTTCAACATTCTAATGTTGCTTACAATTGTCGTGTGGTTT
TTTATAATAAAAAAATTATTCTCATTAGACCTAAAATGATGATGTGTGATGATGGAAACT
ATCGTGAATCTCGTTGGTTCACAGCATGGACAAAAGTAAGAAAAGTTGAAGATTATTTCC
TTCCACGAATGATTGCGATAGCTACAAATCAACTTACGGTTCCTTTTGGTGATGGTGTGA
TTTCAACAAGAGAAACTTGCATTGGTTTTGAAATATGCGAAGAATTATGGAATCCACGTT
CAACACATATCGATTTGGGGTTAGCAGGTGTTGAAATTATTGCCAATGGCAGTAGCAGTT
ATATTCAACTACGTAAAGCTCATATCACAGCAGATTTAATAAAAAATGCAACATATAAAG
CTGGTGGTGCTTATCTTTTTTCAAATTTACGTGGATGTGATGGACAACGAGTTTGGTGGG
GCGGAAATTCAGCAATTGGATTAAATGGTGAAATAATTGCAAAAAGCAAGCAATTTTCAT
TGTTGGATGTTGAAGTTACAGTAGCAACTATTGATTTAGAAGACATCAGAAGTTATCGAA
TGGCTTTAAGATCACGTTGCACTTTTGGAGCTTCTAGTCCAGTTTATCCAAGAATTGCTG
TCGATTGTGAACTTTCAAGTCGTCGCGATGTTTTGATTCCTGTGAATTTACCATTAGAAT
GGATTAATCATACTCCAGAAGAAGAAATTGCTATGGGTCCTGCATGCTGGTTGTGGGATT
ATCTTCGTCGTTCAGGTCAAGGTGGTTATTTTTTGCCATTGTCAGGCGGTATTGATTCTT
GCAGTACAGCATGCATTGTGTATAACATGTGTCAAATGGTAGTTGATGCAATTCAAAATG
GCGAAGATGACGTTTTACGTGATGTAAGAAAAATCACAGCTGATCCAGAATTTTCACCTC
AAACAGCAGCTGAATTGTGCAATAAAATATTTGTGACTTGTTTTATGGGATCAAAAAATT
CTAGTAAAGAAACAAAAAATCGTGCAGCAGTTTTAGCAAATGAAATTGGAAGTTATCATT
TGGAACTCAATATTGACAGTGTTTTTGACACAATTTTATCAGTTTTCAAAGCAGTCACTG
GTTTTATACCAAAATATCGGGTTCAAGGTGGTTCCACAAGAGAAAATCTTGCATTGCAAA
ACATTCAAGC
>g17053.t7 Gene=g17053 Length=412
MMSPVCENMLIDVGMPVQHSNVAYNCRVVFYNKKIILIRPKMMMCDDGNYRESRWFTAWT
KVRKVEDYFLPRMIAIATNQLTVPFGDGVISTRETCIGFEICEELWNPRSTHIDLGLAGV
EIIANGSSSYIQLRKAHITADLIKNATYKAGGAYLFSNLRGCDGQRVWWGGNSAIGLNGE
IIAKSKQFSLLDVEVTVATIDLEDIRSYRMALRSRCTFGASSPVYPRIAVDCELSSRRDV
LIPVNLPLEWINHTPEEEIAMGPACWLWDYLRRSGQGGYFLPLSGGIDSCSTACIVYNMC
QMVVDAIQNGEDDVLRDVRKITADPEFSPQTAAELCNKIFVTCFMGSKNSSKETKNRAAV
LANEIGSYHLELNIDSVFDTILSVFKAVTGFIPKYRVQGGSTRENLALQNIQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g17053.t7 | CDD | cd07570 | GAT_Gln-NAD-synth | 6 | 214 | 0.000 |
| 7 | g17053.t7 | Gene3D | G3DSA:3.60.110.10 | - | 3 | 216 | 0.000 |
| 6 | g17053.t7 | Gene3D | G3DSA:3.40.50.620 | HUPs | 255 | 412 | 0.000 |
| 2 | g17053.t7 | PANTHER | PTHR23090:SF9 | GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE | 3 | 412 | 0.000 |
| 3 | g17053.t7 | PANTHER | PTHR23090 | NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE | 3 | 412 | 0.000 |
| 9 | g17053.t7 | PIRSF | PIRSF006630 | NADS_GAT | 2 | 412 | 0.000 |
| 1 | g17053.t7 | Pfam | PF00795 | Carbon-nitrogen hydrolase | 4 | 209 | 0.000 |
| 8 | g17053.t7 | ProSiteProfiles | PS50263 | Carbon-nitrogen hydrolase domain profile. | 1 | 202 | 26.213 |
| 5 | g17053.t7 | SUPERFAMILY | SSF56317 | Carbon-nitrogen hydrolase | 8 | 214 | 0.000 |
| 4 | g17053.t7 | SUPERFAMILY | SSF52402 | Adenine nucleotide alpha hydrolases-like | 248 | 412 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006807 | nitrogen compound metabolic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0004359 | glutaminase activity | MF |
| GO:0003952 | NAD+ synthase (glutamine-hydrolyzing) activity | MF |
| GO:0005737 | cytoplasm | CC |
| GO:0009435 | NAD biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
