General information for g17053.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamine-dependent NAD(+) synthetase.
Parent gene
- The parent gene g17053 is located on chr_4: 12322973..12325635 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17053 | g17053.t8 | isoform | g17053.t8 | 12322667 | 12323816 |
| chr_4 | g17053 | g17053.t8 | exon | g17053.t8.exon1 | 12322667 | 12323334 |
| chr_4 | g17053 | g17053.t8 | cds | g17053.t8.CDS1 | 12322667 | 12323334 |
| chr_4 | g17053 | g17053.t8 | exon | g17053.t8.exon2 | 12323403 | 12323617 |
| chr_4 | g17053 | g17053.t8 | cds | g17053.t8.CDS2 | 12323403 | 12323617 |
| chr_4 | g17053 | g17053.t8 | exon | g17053.t8.exon3 | 12323671 | 12323816 |
| chr_4 | g17053 | g17053.t8 | cds | g17053.t8.CDS3 | 12323671 | 12323816 |
| chr_4 | g17053 | g17053.t8 | TSS | g17053.t8 | NA | NA |
| chr_4 | g17053 | g17053.t8 | TTS | g17053.t8 | NA | NA |
Sequences
>g17053.t8 Gene=g17053 Length=1029
ATGGGTCGAAAAGTAACAGTTGCAGTATCTACATTGAGTCAATGGGCTTTGGATTTTGAA
GGCAACATGGCAAGAATTATACAATCAATTTTGGAAGCACGTGAACTTGGAGCTCTTTAT
CGTACTGGACCAGAATTGGAAATTACTGGTTACAGTTGTGAAGATCATTTTTATGAGCCA
GATACATTCTTGCATTCATGGGAAGTTCTTTTGCAAATTATGATGTCACCAGTTTGTGAA
AATATGCTGATTGATGTTGGAATGCCAGTTCAACATTCTAATGTTGCTTACAATTGTCGT
GTGGTTTTTTATAATAAAAAAATTATTCTCATTAGACCTAAAATGATGATGTGTGATGAT
GGAAACTATCGTGAATCTCGTTGGTTCACAGCATGGACAAAAGTAAGAAAAGTTGAAGAT
TATTTCCTTCCACGAATGATTGCGATAGCTACAAATCAACTTACGGTTCCTTTTGGTGAT
GGTGTGATTTCAACAAGAGAAACTTGCATTGGTTTTGAAATATGCGAAGAATTATGGAAT
CCACGTTCAACACATATCGATTTGGGGTTAGCAGGTGTTGAAATTATTGCCAATGGCAGT
AGCAGTTATATTCAACTACGTAAAGCTCATATCACAGCAGATTTAATAAAAAATGCAACA
TATAAAGCTGGTGGTGCTTATCTTTTTTCAAATTTACGTGGATGTGATGGACAACGAGTT
TGGTGGGGCGGAAATTCAGCAATTGGATTAAATGGTGAAATAATTGCAAAAAGCAAGCAA
TTTTCATTGTTGGATGTTGAAGTTACAGTAGCAACTATTGATTTAGAAGACATCAGAAGT
TATCGAATGGCTTTAAGATCACGTTGCACTTTTGGAGCTTCTAGTCCAGTTTATCCAAGA
ATTGCTGTCGATTGTGAACTTTCAAGTCGTCGCGATGTTTTGATTCCTGTGAATTTACCA
TTAGAATGGATTAATCATACTCCAGAAGAAGAAATTGCTATGGGTCCTGCATGCTGGTTG
TGGGATTAT
>g17053.t8 Gene=g17053 Length=343
MGRKVTVAVSTLSQWALDFEGNMARIIQSILEARELGALYRTGPELEITGYSCEDHFYEP
DTFLHSWEVLLQIMMSPVCENMLIDVGMPVQHSNVAYNCRVVFYNKKIILIRPKMMMCDD
GNYRESRWFTAWTKVRKVEDYFLPRMIAIATNQLTVPFGDGVISTRETCIGFEICEELWN
PRSTHIDLGLAGVEIIANGSSSYIQLRKAHITADLIKNATYKAGGAYLFSNLRGCDGQRV
WWGGNSAIGLNGEIIAKSKQFSLLDVEVTVATIDLEDIRSYRMALRSRCTFGASSPVYPR
IAVDCELSSRRDVLIPVNLPLEWINHTPEEEIAMGPACWLWDY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g17053.t8 | CDD | cd07570 | GAT_Gln-NAD-synth | 6 | 287 | 0.000 |
| 5 | g17053.t8 | Gene3D | G3DSA:3.60.110.10 | - | 2 | 289 | 0.000 |
| 2 | g17053.t8 | PANTHER | PTHR23090:SF9 | GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE | 1 | 343 | 0.000 |
| 3 | g17053.t8 | PANTHER | PTHR23090 | NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE | 1 | 343 | 0.000 |
| 1 | g17053.t8 | Pfam | PF00795 | Carbon-nitrogen hydrolase | 6 | 282 | 0.000 |
| 6 | g17053.t8 | ProSiteProfiles | PS50263 | Carbon-nitrogen hydrolase domain profile. | 5 | 275 | 39.739 |
| 4 | g17053.t8 | SUPERFAMILY | SSF56317 | Carbon-nitrogen hydrolase | 3 | 287 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006807 | nitrogen compound metabolic process | BP |
| GO:0004359 | glutaminase activity | MF |
| GO:0003952 | NAD+ synthase (glutamine-hydrolyzing) activity | MF |
| GO:0005737 | cytoplasm | CC |
| GO:0009435 | NAD biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
