General information for g17114.t18
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Lysosomal aspartic protease.
Parent gene
- The parent gene g17114 is located on chr_4: 12519041..12520450 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17114 | g17114.t18 | TTS | g17114.t18 | 12518088 | 12518088 |
| chr_4 | g17114 | g17114.t18 | isoform | g17114.t18 | 12518210 | 12520362 |
| chr_4 | g17114 | g17114.t18 | exon | g17114.t18.exon1 | 12518210 | 12518297 |
| chr_4 | g17114 | g17114.t18 | exon | g17114.t18.exon2 | 12518554 | 12518963 |
| chr_4 | g17114 | g17114.t18 | cds | g17114.t18.CDS1 | 12518944 | 12518963 |
| chr_4 | g17114 | g17114.t18 | exon | g17114.t18.exon3 | 12519053 | 12519251 |
| chr_4 | g17114 | g17114.t18 | cds | g17114.t18.CDS2 | 12519053 | 12519251 |
| chr_4 | g17114 | g17114.t18 | exon | g17114.t18.exon4 | 12519796 | 12519986 |
| chr_4 | g17114 | g17114.t18 | cds | g17114.t18.CDS3 | 12519796 | 12519986 |
| chr_4 | g17114 | g17114.t18 | exon | g17114.t18.exon5 | 12520047 | 12520222 |
| chr_4 | g17114 | g17114.t18 | cds | g17114.t18.CDS4 | 12520047 | 12520222 |
| chr_4 | g17114 | g17114.t18 | exon | g17114.t18.exon6 | 12520289 | 12520362 |
| chr_4 | g17114 | g17114.t18 | cds | g17114.t18.CDS5 | 12520289 | 12520362 |
| chr_4 | g17114 | g17114.t18 | TSS | g17114.t18 | 12520391 | 12520391 |
Sequences
>g17114.t18 Gene=g17114 Length=1138
ATGTCAAAAAAGTTTAGTGCAACAATTTTTCTTCTCCTTCAGCTTTTGCTGATTGTAAAT
GGAAAACTATTGAGAATTCCTTTGTATAAATTTGAATCAGCAAGACGCCATTTTCAAACC
GTAGGAACAGATCTCACACAAGTTCGTCTTGCAAATGTTCAAGGACCAACACCAGAACCA
TTGTCAAATTATCTTGATGCCCAATATTATGGACCAATTACAATTGGAAGTCCACCACAA
AGTTTCAAAGTTGTTTTTGACACTGGAAGTTCAAATTTATGGGTTCCATCAAAATCTTGT
TCACTTATGAACATTGCCTGTCTTATGCATAACAAATATGATTCGAAAAAAAGTTCAACA
TACGAGAAAAATGGAACAAAATTTCATATCGAATACGGTAGCGGTTCACTAAGTGGCTTC
TTGTCAACTGATACTGTCGGTATTGGTGATTTAAAAATTCAAAAGCAAACATTTGCAGAA
GCAGTTTCAGAACCCGGTTTAGTTTTTGTTGCTGCTAAATTTGATGGAATTCTTGGATTA
GGCTACAAATCAATTTCAGTTGATGGAGTTGAACCAGTTTTCTATAACATGTACAATCAA
GGACTCATTTCAGAGCCAATTTTCTCATTTTATTTGAATCATTATTTTTGGTGGAAGTGA
TCCAGATCATTATGAAGGTGATTTCACTTATCTCCCAGTCAATCGCAAAGCATACTGGCA
ATTCAAAATGGATGGAGTGAAAATTGGTGACAAAACTTTCTGTAATGGTGGATGCCAAGC
AATTGCTGACACTGGAACATCACTCATTGCTGGACCAACTGATGAAGTTACAGCAATTAA
CAAAGCCATCGGTGGAACACCAATTTTAAATGGACAATACATGGTTAATTGTGACATGAT
ACCAAATTTGCCTACAATTAAATTCACTTTGGGTGGCAAGGAATTTGCACTTGATGGATC
TGATTATATTTTAAGAATCGCTCAAATGGGTAAAACTATTTGCTTGTCTGGTTTCATGGG
AATTAATATTCCACCACCAAATGGACCACTATGGATTCTCGGTGATGTTTTTATTGGAAA
ATACTACACAGAATTTGATTTTGGCAATGACCGTGTTGGTTTTGCTGTCACAAAATAG
>g17114.t18 Gene=g17114 Length=219
MSKKFSATIFLLLQLLLIVNGKLLRIPLYKFESARRHFQTVGTDLTQVRLANVQGPTPEP
LSNYLDAQYYGPITIGSPPQSFKVVFDTGSSNLWVPSKSCSLMNIACLMHNKYDSKKSST
YEKNGTKFHIEYGSGSLSGFLSTDTVGIGDLKIQKQTFAEAVSEPGLVFVAAKFDGILGL
GYKSISVDGVEPVFYNMYNQGLISEPIFSFYLNHYFWWK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g17114.t18 | Gene3D | G3DSA:2.40.70.10 | Acid Proteases | 25 | 216 | 7.7E-66 |
| 3 | g17114.t18 | PANTHER | PTHR47966:SF19 | NAPSIN A ASPARTIC PEPTIDASE | 16 | 213 | 2.6E-79 |
| 4 | g17114.t18 | PANTHER | PTHR47966 | BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED | 16 | 213 | 2.6E-79 |
| 6 | g17114.t18 | PRINTS | PR00792 | Pepsin (A1) aspartic protease family signature | 75 | 95 | 1.4E-7 |
| 5 | g17114.t18 | PRINTS | PR00792 | Pepsin (A1) aspartic protease family signature | 183 | 196 | 1.4E-7 |
| 1 | g17114.t18 | Pfam | PF07966 | A1 Propeptide | 23 | 49 | 1.0E-4 |
| 2 | g17114.t18 | Pfam | PF00026 | Eukaryotic aspartyl protease | 68 | 213 | 1.9E-64 |
| 11 | g17114.t18 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 12 | g17114.t18 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 13 | g17114.t18 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 17 | - |
| 14 | g17114.t18 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 21 | - |
| 10 | g17114.t18 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 219 | - |
| 16 | g17114.t18 | ProSitePatterns | PS00141 | Eukaryotic and viral aspartyl proteases active site. | 84 | 95 | - |
| 17 | g17114.t18 | ProSiteProfiles | PS51767 | Peptidase family A1 domain profile. | 69 | 219 | 42.49 |
| 7 | g17114.t18 | SUPERFAMILY | SSF50630 | Acid proteases | 9 | 214 | 4.98E-70 |
| 8 | g17114.t18 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 21 | - |
| 15 | g17114.t18 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006508 | proteolysis | BP |
| GO:0004190 | aspartic-type endopeptidase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
