General information for g17163.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 9e2.
Parent gene
- The parent gene g17163 is located on chr_4: 12689302..12690335 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17163 | g17163.t1 | TTS | g17163.t1 | 12688267 | 12688267 |
| chr_4 | g17163 | g17163.t1 | isoform | g17163.t1 | 12688307 | 12690335 |
| chr_4 | g17163 | g17163.t1 | exon | g17163.t1.exon1 | 12688307 | 12688492 |
| chr_4 | g17163 | g17163.t1 | cds | g17163.t1.CDS1 | 12688307 | 12688492 |
| chr_4 | g17163 | g17163.t1 | exon | g17163.t1.exon2 | 12688542 | 12688970 |
| chr_4 | g17163 | g17163.t1 | cds | g17163.t1.CDS2 | 12688542 | 12688970 |
| chr_4 | g17163 | g17163.t1 | exon | g17163.t1.exon3 | 12689295 | 12690072 |
| chr_4 | g17163 | g17163.t1 | cds | g17163.t1.CDS3 | 12689295 | 12690072 |
| chr_4 | g17163 | g17163.t1 | exon | g17163.t1.exon4 | 12690124 | 12690335 |
| chr_4 | g17163 | g17163.t1 | cds | g17163.t1.CDS4 | 12690124 | 12690335 |
| chr_4 | g17163 | g17163.t1 | TSS | g17163.t1 | 12690401 | 12690401 |
Sequences
>g17163.t1 Gene=g17163 Length=1605
ATGGACACTTATTTATTAATCATTTGGGCTGTCATTTTTCTTGGATATTTCATTTACAAA
AAAGCAACAAAAAATTTAAAATTTTTTGAAAAGCATGGAATTCCATATGAAAAACCACTG
CCATTTTTCGGCAATTCATTAAAACTTTTAATGAAAAAGGAAAATATGATTGAATCTATT
GTGAATCCATATGAAAAATTCAAAAATAGTTTTGTTTATGGAGTTTTTGGAATGAACATT
CCCAATTTTGTTATCAGTGATCCAGAATTGATAAAACAAATAACAATTAAAGATTTTGAT
AGCTTTGTCAATCATGATAAGAGCTTTAATGAAGATGTCGATAAATTTTCAAGCAAAATG
TTGTTTACATTGGTAGACGAGAAATGGCGGCATATGAGGACAGTTTTAAGTCCAATTTTT
ACAAGTTCCAAAATGAAAATGATGTTTGGAATTTTATCAGAATGTGCAAATGAATTTGTT
GAACATCATGAAGAAAAAGCTAAGCATGGAAAAGTCATTGTAAATTGTGTTGAAACTTAT
CCACGTTACACTGTTGATGGAATTTCTACTGCTGTTCTTGGATTTAAAGGTGACTGCATT
AAAAATGAAGACAGCAAGCTCTATAAATTTGCAATGCGAATGAGAAAGCCAACATTTATA
ACAAACATAAAAATTATGATTTTTCTTATATCTCGTTGGTTTTATGTCAAACTTGGCTTA
CAAATGACATTGAAAGAAGTTTATGATTTTTTCTATAATGCAATCATTAAAGTCATGAAT
GAACGAGAAGAAAAAGGAATTTTTCGACCTGATGTTGTGCAATTGCTGCTTCAAGCAAGA
CAAGGACAATTGCAAAAAGAAAGTGACATCAATGAAAAAGAATTGTCAAACTTTTCTGCT
AATATTGAATATGATACTGGTGCTCAAAATAAGAAGATAACAAATTGGACCGATGAACAT
TATATGGCTCAGGGATTTTTATTCTTTGCAGCTGGATTCGACACAACAAACACTTTGCTT
CAAATAACCAGTTATTGTTTAGCAAAAAATAAAGAAGTTCAAGATGAATTAATAAGAGAA
ATTGATGATGTTCTTCAAGAACTTGATGCAAAGCCAATCACATATGAAGCACTGCATAAA
ATGAAATATTTGGATATGGTAATTTCTGAAGCATTAAGAATTTGGCCACCAGCATTTGCA
ACATCAAGACAATGTAATAAAGATTATGATTTAAAATTAAAAGATGGAAAAGTCATTAAA
ATTAAGGATGGTGATCTCATTTTGATACCAACATATGCAATTCATCATGATGAAAGATTT
TTCACTAATCCAGAAAAATTTGATCCACTTCGATTTAGTGATGAAAATAAAGAATCTATT
GCTGTTGGGTCATATATACCTTTTGGATTAGGTCCAAGAGCTTGTATTGGAAGTCGTTTT
GCATTAATGGAAGCAAAATTGCTCATTTTTAATGTTATGAGCAAATTCACAATGGAAGTT
TGTGATAAAACAACAGAAAAGCTTGAATTGGTTCCATCAATGACAGAAGTTACTTTTAAA
AATGGAATTTTTGTTGAATATCGTCCACGAAAGAAGAACAACTAG
>g17163.t1 Gene=g17163 Length=534
MDTYLLIIWAVIFLGYFIYKKATKNLKFFEKHGIPYEKPLPFFGNSLKLLMKKENMIESI
VNPYEKFKNSFVYGVFGMNIPNFVISDPELIKQITIKDFDSFVNHDKSFNEDVDKFSSKM
LFTLVDEKWRHMRTVLSPIFTSSKMKMMFGILSECANEFVEHHEEKAKHGKVIVNCVETY
PRYTVDGISTAVLGFKGDCIKNEDSKLYKFAMRMRKPTFITNIKIMIFLISRWFYVKLGL
QMTLKEVYDFFYNAIIKVMNEREEKGIFRPDVVQLLLQARQGQLQKESDINEKELSNFSA
NIEYDTGAQNKKITNWTDEHYMAQGFLFFAAGFDTTNTLLQITSYCLAKNKEVQDELIRE
IDDVLQELDAKPITYEALHKMKYLDMVISEALRIWPPAFATSRQCNKDYDLKLKDGKVIK
IKDGDLILIPTYAIHHDERFFTNPEKFDPLRFSDENKESIAVGSYIPFGLGPRACIGSRF
ALMEAKLLIFNVMSKFTMEVCDKTTEKLELVPSMTEVTFKNGIFVEYRPRKKNN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g17163.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 534 | 1.0E-121 |
| 2 | g17163.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 7 | 525 | 7.4E-149 |
| 7 | g17163.t1 | PRINTS | PR00463 | E-class P450 group I signature | 320 | 337 | 3.3E-16 |
| 9 | g17163.t1 | PRINTS | PR00385 | P450 superfamily signature | 331 | 348 | 6.3E-11 |
| 4 | g17163.t1 | PRINTS | PR00463 | E-class P450 group I signature | 340 | 366 | 3.3E-16 |
| 11 | g17163.t1 | PRINTS | PR00385 | P450 superfamily signature | 386 | 397 | 6.3E-11 |
| 6 | g17163.t1 | PRINTS | PR00463 | E-class P450 group I signature | 430 | 454 | 3.3E-16 |
| 5 | g17163.t1 | PRINTS | PR00463 | E-class P450 group I signature | 465 | 475 | 3.3E-16 |
| 8 | g17163.t1 | PRINTS | PR00385 | P450 superfamily signature | 466 | 475 | 6.3E-11 |
| 3 | g17163.t1 | PRINTS | PR00463 | E-class P450 group I signature | 475 | 498 | 3.3E-16 |
| 10 | g17163.t1 | PRINTS | PR00385 | P450 superfamily signature | 475 | 486 | 6.3E-11 |
| 1 | g17163.t1 | Pfam | PF00067 | Cytochrome P450 | 35 | 511 | 7.6E-80 |
| 16 | g17163.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 18 | g17163.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 22 | - |
| 14 | g17163.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 23 | 218 | - |
| 17 | g17163.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 219 | 236 | - |
| 15 | g17163.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 237 | 534 | - |
| 21 | g17163.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 468 | 477 | - |
| 12 | g17163.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 34 | 530 | 4.85E-103 |
| 20 | g17163.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 19 | - |
| 19 | g17163.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 219 | 236 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K17861 (cytochrome P450 family 13)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
