Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Pyrimidodiazepine synthase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g17215 g17215.t14 isoform g17215.t14 12845541 12846442
chr_4 g17215 g17215.t14 exon g17215.t14.exon1 12845541 12846067
chr_4 g17215 g17215.t14 TSS g17215.t14 12845542 12845542
chr_4 g17215 g17215.t14 cds g17215.t14.CDS1 12845593 12846067
chr_4 g17215 g17215.t14 exon g17215.t14.exon2 12846258 12846442
chr_4 g17215 g17215.t14 cds g17215.t14.CDS2 12846258 12846442
chr_4 g17215 g17215.t14 TTS g17215.t14 12846528 12846528

Sequences

>g17215.t14 Gene=g17215 Length=712
AGGTTCATCCCAACCAACATTTCCTGACAATGAAATTCTTCGACTTTATTCAATGAAATA
TTGTCCTTATGCTCATCGAGCTCATTTGGTACTTGATGCAAAAAACATCCCTTATCACAC
TGTTTTTATTAATTTAACTGAGAAACCAGAATGGCTTTCCAAAGTTTCAGCACTCACAAA
AGTTCCAGCTTTAGAATTACCAGGAGTTAAAGGAGATTCTTTGATTGAATCTTTAATTAT
TTGTGACTACATTGATGAAAAATATCCACAAAATTCTTTGAATTCTAAAGATCCATTAGA
GAAAGCGCGTGACAGAATTTTAGTGCAAAAATTTGAAACTATATCACCAGTTTTTGCTCG
TTTTGTATTTTGGAAGAAAGAAGAAGATAAAGGTGAACTTTTGAAAAAACTTTATGCAGG
ATTGAAGAATTTTGATGACGAATTAAATGAAAGAAATTCCAAATTCTTTGGTGGTGAAAA
ACCTGGCATGACTGATTATATGATTTGGCCTTGGTTTGAAAGATTTGAAGTTTTAAATTT
CATGTTGGAAGAAAAATTCAAATTGGACAAAGATCGTTTTCCAAAATTGTCAGAATGGTT
TGATGGAATGATGAATGATGAAGCTGTAAGAAGAAATTTCATAGACATTGACGATCATTA
TAGATTTGTGACAACAAGAGATTATGACAATGCTGCTGAAAATAAATTGTAA

>g17215.t14 Gene=g17215 Length=219
MKYCPYAHRAHLVLDAKNIPYHTVFINLTEKPEWLSKVSALTKVPALELPGVKGDSLIES
LIICDYIDEKYPQNSLNSKDPLEKARDRILVQKFETISPVFARFVFWKKEEDKGELLKKL
YAGLKNFDDELNERNSKFFGGEKPGMTDYMIWPWFERFEVLNFMLEEKFKLDKDRFPKLS
EWFDGMMNDEAVRRNFIDIDDHYRFVTTRDYDNAAENKL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g17215.t14 Gene3D G3DSA:3.40.30.10 Glutaredoxin 1 208 0.000
8 g17215.t14 Gene3D G3DSA:1.20.1050.10 - 77 197 0.000
3 g17215.t14 PANTHER PTHR43968 - 1 208 0.000
5 g17215.t14 PRINTS PR01625 Omega-class glutathione S-transferase signature 71 85 0.000
4 g17215.t14 PRINTS PR01625 Omega-class glutathione S-transferase signature 138 157 0.000
2 g17215.t14 Pfam PF13417 Glutathione S-transferase, N-terminal domain 1 74 0.000
1 g17215.t14 Pfam PF00043 Glutathione S-transferase, C-terminal domain 97 189 0.000
11 g17215.t14 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 1 75 17.697
10 g17215.t14 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 80 209 17.747
12 g17215.t14 SFLD SFLDG00358 Main (cytGST) 2 188 0.000
13 g17215.t14 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 2 188 0.000
6 g17215.t14 SUPERFAMILY SSF52833 Thioredoxin-like 2 95 0.000
7 g17215.t14 SUPERFAMILY SSF47616 GST C-terminal domain-like 79 208 0.000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004364 glutathione transferase activity MF
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF
GO:0005737 cytoplasm CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values