Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Pyrimidodiazepine synthase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g17215 g17215.t3 isoform g17215.t3 12844525 12846062
chr_4 g17215 g17215.t3 exon g17215.t3.exon1 12844525 12844598
chr_4 g17215 g17215.t3 TSS g17215.t3 12844546 12844546
chr_4 g17215 g17215.t3 exon g17215.t3.exon2 12844950 12845049
chr_4 g17215 g17215.t3 cds g17215.t3.CDS1 12845019 12845049
chr_4 g17215 g17215.t3 exon g17215.t3.exon3 12845543 12846062
chr_4 g17215 g17215.t3 cds g17215.t3.CDS2 12845543 12846060
chr_4 g17215 g17215.t3 TTS g17215.t3 12846528 12846528

Sequences

>g17215.t3 Gene=g17215 Length=694
AACTTGACTCAAGATTGTGTTCTTTTAGAGAATTTTACAAAAAAAAGTTTGAAAGAATTT
TTTGATATCAACAGGTATAAAAATATAAATACCATACCAAAAACCATTCAGACATAAATT
GAATGTCAGCAAGAAGACTAGCAATGACTATTAAAACACTTCATTTAACAAAAGGTTCAT
CCCAACCAACATTTCCTGACAATGAAATTCTTCGACTTTATTCAATGAAATATTGTCCTT
ATGCTCATCGAGCTCATTTGGTACTTGATGCAAAAAACATCCCTTATCACACTGTTTTTA
TTAATTTAACTGAGAAACCAGAATGGCTTTCCAAAGTTTCAGCACTCACAAAAGTTCCAG
CTTTAGAATTACCAGGAGTTAAAGGAGATTCTTTGATTGAATCTTTAATTATTTGTGACT
ACATTGATGAAAAATATCCACAAAATTCTTTGAATTCTAAAGATCCATTAGAGAAAGCGC
GTGACAGAATTTTAGTGCAAAAATTTGAAACTATATCACCAGTTTTTGCTCGTTTTGTAT
TTTGGAAGAAAGAAGAAGATAAAGGTGAACTTTTGAAAAAACTTTATGCAGGATTGAAGA
ATTTTGATGACGAATTAAATGAAAGAAATTCCAAATTCTTTGGTGGTGAAAAACCTGGCA
TGACTGATTATATGATTTGGCCTTGGTTTGAAAG

>g17215.t3 Gene=g17215 Length=183
MTIKTLHLTKGSSQPTFPDNEILRLYSMKYCPYAHRAHLVLDAKNIPYHTVFINLTEKPE
WLSKVSALTKVPALELPGVKGDSLIESLIICDYIDEKYPQNSLNSKDPLEKARDRILVQK
FETISPVFARFVFWKKEEDKGELLKKLYAGLKNFDDELNERNSKFFGGEKPGMTDYMIWP
WFE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g17215.t3 Gene3D G3DSA:3.40.30.10 Glutaredoxin 19 103 0.000
8 g17215.t3 Gene3D G3DSA:1.20.1050.10 - 105 183 0.000
2 g17215.t3 PANTHER PTHR43968 - 6 183 0.000
5 g17215.t3 PRINTS PR01625 Omega-class glutathione S-transferase signature 21 36 0.000
4 g17215.t3 PRINTS PR01625 Omega-class glutathione S-transferase signature 98 112 0.000
3 g17215.t3 PRINTS PR01625 Omega-class glutathione S-transferase signature 165 183 0.000
1 g17215.t3 Pfam PF13417 Glutathione S-transferase, N-terminal domain 25 101 0.000
11 g17215.t3 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 21 102 19.260
10 g17215.t3 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 107 183 13.238
12 g17215.t3 SFLD SFLDG00358 Main (cytGST) 23 183 0.000
13 g17215.t3 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 23 183 0.000
6 g17215.t3 SUPERFAMILY SSF52833 Thioredoxin-like 24 122 0.000
7 g17215.t3 SUPERFAMILY SSF47616 GST C-terminal domain-like 106 182 0.000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004364 glutathione transferase activity MF
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF
GO:0005737 cytoplasm CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values