General information for g17221.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Plasma kallikrein.
Parent gene
- The parent gene g17221 is located on chr_4: 12861807..12865322 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17221 | g17221.t1 | isoform | g17221.t1 | 12861807 | 12865322 |
| chr_4 | g17221 | g17221.t1 | exon | g17221.t1.exon1 | 12861807 | 12862641 |
| chr_4 | g17221 | g17221.t1 | cds | g17221.t1.CDS1 | 12861807 | 12862641 |
| chr_4 | g17221 | g17221.t1 | exon | g17221.t1.exon2 | 12863910 | 12863924 |
| chr_4 | g17221 | g17221.t1 | cds | g17221.t1.CDS2 | 12863910 | 12863924 |
| chr_4 | g17221 | g17221.t1 | exon | g17221.t1.exon3 | 12863988 | 12863994 |
| chr_4 | g17221 | g17221.t1 | cds | g17221.t1.CDS3 | 12863988 | 12863994 |
| chr_4 | g17221 | g17221.t1 | exon | g17221.t1.exon4 | 12864298 | 12864909 |
| chr_4 | g17221 | g17221.t1 | cds | g17221.t1.CDS4 | 12864298 | 12864909 |
| chr_4 | g17221 | g17221.t1 | exon | g17221.t1.exon5 | 12865031 | 12865322 |
| chr_4 | g17221 | g17221.t1 | cds | g17221.t1.CDS5 | 12865031 | 12865322 |
| chr_4 | g17221 | g17221.t1 | TSS | g17221.t1 | NA | NA |
| chr_4 | g17221 | g17221.t1 | TTS | g17221.t1 | NA | NA |
Sequences
>g17221.t1 Gene=g17221 Length=1761
ATGAATCAATTTTCAATTCTTCTTTTCTGTGCAATTTTTGTATCAAATATTTTACAAATT
TCAACTGAAATTATCAAAAATAAAATCGAACTGTTAGAAATTGATGATCAAAATTTTATA
AACAAAAAAGAACCTGTAAGTGGGATTGGATTTATTGAAACTTTTAATGGAACAAGAGAA
AGTAAACCTAGTGAATGGCCTTGGCATGTTGCATTATATAGAAAAATAAATAATAAAAAT
TCAAACTATTTTTGTGGAGCTTCTTTGATTTCTGATCAACATTTATTAACAGCTGCTCAT
TGTTTTAAATATTATGAAATTTTTGATCAAATTTCTGACTATCATGCAATTCTTGGAGTT
TATAATTTAAGCAATAATAATGAAGACTCAAGAATGTATCGAAATTTTTCGTCAATTGAA
ATTCATGAAGAATTTAAACTTCTTATAAATGATTCATTAAGTAATGCTGATATTGCTATT
ATAAAAATGAACAAAAAAGTTAAATTTTCTGACCTCATAAAACCTGTCAGACTTCCATCT
CTTAACATGCAAAAAACTGCTCTTATTGGAACAATCGTTGGCTATGGCGCTACAAATGGA
AGTGAAAAACTAACAAATATACTACAAAAAGGTGAACTAAAAATTATCAATATCGATGAA
TGTCAAAAAAAAGAACGAGCATATACTGGAATTTTATCATCAAGAAGTTTCTGTGCTGGT
GAAACTGATGCAACTCCATGTAAAGGCGATTCCGGTGGTGGAATTTTTGTTAAGAACAAT
CGAACTGGAGAATTTGAAATTGTTGGAATTGCCTCAATTAGACTTACTAAAATTGAAAGT
TGCGATCCAAATGATTATGCAATTTTTGTAAATGTTTCAAAATTTTTTGATTGGATTTGG
TCAAATAGCAAATTTCACACGGGCGGGACTTCAAAGAATCCAACAAAAATTATCATTCTG
ACAATTTTCCTTCTTTTTCTCACTCAAAAATCTTTTTCTTTACGCCTCGATTGCAATTTT
GTAAATTTTTTCTGGGCAACACTTTCTCATACTTATTCATGTGATGCAACTATAGAAAAA
TGTGTAAATGACGATGACGACACTTGTGAATCAAACAGACCAGAAGTCAATGCAATTTAT
GGCTTACATGAAGAAAATGAAAGAAATAAATTGAGACAAAGTGATGAAACTGTTCATGGA
CTTTTAATTGTTCATCAGCGGCATGTGACAAAATTTCCTCGAAATATTGAAGGATATTTT
CCAAATTTACTTGGTATTGATATGGAAGGTTGCGGATTGACGACAATTTCAGCTAGAGAT
TTAGCGCCATTCAAGAATCTTCGACAAATTTCATTGGAAGACAATAAAATCACAGAACTT
GCATCAAATTTATTTCATCATAATCCAAACTTGCGTAGAATTGACTTGGAAGGGAACAAC
ATTATTCACATTGGTCTTGATATTTTTAGACATTTAAATCAACTCGAAATTGTTTATTTA
CGTTACAATCCATGCACAAATGATAATTCTCTTGCACTCAATAGAGCTGAAATGAAAGTC
CTCGAATGGGAAATTCAAGTCGAATGTCCTGCAACTTTAAATATGCTTTCACATGCACTT
GTTGGATCGAAAACTTTTAAGGAAACTTTCGAAAGATTGTCTGAAGAATATTTTGGAATT
GTTCGTGAAGAAATGATGGAACAAATTAAAGACTTAAAGAAACAATTTGAAAATTTGAAG
AAAAATAGTAGAATAAGATAA
>g17221.t1 Gene=g17221 Length=586
MNQFSILLFCAIFVSNILQISTEIIKNKIELLEIDDQNFINKKEPVSGIGFIETFNGTRE
SKPSEWPWHVALYRKINNKNSNYFCGASLISDQHLLTAAHCFKYYEIFDQISDYHAILGV
YNLSNNNEDSRMYRNFSSIEIHEEFKLLINDSLSNADIAIIKMNKKVKFSDLIKPVRLPS
LNMQKTALIGTIVGYGATNGSEKLTNILQKGELKIINIDECQKKERAYTGILSSRSFCAG
ETDATPCKGDSGGGIFVKNNRTGEFEIVGIASIRLTKIESCDPNDYAIFVNVSKFFDWIW
SNSKFHTGGTSKNPTKIIILTIFLLFLTQKSFSLRLDCNFVNFFWATLSHTYSCDATIEK
CVNDDDDTCESNRPEVNAIYGLHEENERNKLRQSDETVHGLLIVHQRHVTKFPRNIEGYF
PNLLGIDMEGCGLTTISARDLAPFKNLRQISLEDNKITELASNLFHHNPNLRRIDLEGNN
IIHIGLDIFRHLNQLEIVYLRYNPCTNDNSLALNRAEMKVLEWEIQVECPATLNMLSHAL
VGSKTFKETFERLSEEYFGIVREEMMEQIKDLKKQFENLKKNSRIR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g17221.t1 | CDD | cd00190 | Tryp_SPc | 59 | 299 | 4.80508E-56 |
| 12 | g17221.t1 | Coils | Coil | Coil | 562 | 582 | - |
| 11 | g17221.t1 | Gene3D | G3DSA:2.40.10.10 | - | 53 | 304 | 1.5E-56 |
| 10 | g17221.t1 | Gene3D | G3DSA:3.80.10.10 | Ribonuclease Inhibitor | 392 | 531 | 3.0E-20 |
| 3 | g17221.t1 | PANTHER | PTHR24260 | - | 44 | 302 | 2.8E-52 |
| 4 | g17221.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 86 | 101 | 1.2E-8 |
| 6 | g17221.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 153 | 167 | 1.2E-8 |
| 5 | g17221.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 244 | 256 | 1.2E-8 |
| 2 | g17221.t1 | Pfam | PF00089 | Trypsin | 56 | 299 | 1.2E-41 |
| 1 | g17221.t1 | Pfam | PF13855 | Leucine rich repeat | 446 | 504 | 1.6E-10 |
| 14 | g17221.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 15 | g17221.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 16 | g17221.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 14 | - |
| 17 | g17221.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 13 | g17221.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 586 | - |
| 22 | g17221.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 96 | 101 | - |
| 23 | g17221.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 54 | 304 | 25.439 |
| 19 | g17221.t1 | SMART | SM00020 | trypsin_2 | 53 | 299 | 7.9E-51 |
| 20 | g17221.t1 | SMART | SM00369 | LRR_typ_2 | 444 | 467 | 0.22 |
| 21 | g17221.t1 | SMART | SM00369 | LRR_typ_2 | 469 | 491 | 23.0 |
| 8 | g17221.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 55 | 303 | 2.77E-60 |
| 7 | g17221.t1 | SUPERFAMILY | SSF52058 | L domain-like | 402 | 512 | 1.81E-18 |
| 9 | g17221.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
