General information for g17252.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Enteropeptidase.
Parent gene
- The parent gene g17252 is located on chr_4: 12949406..12950963 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17252 | g17252.t1 | TTS | g17252.t1 | 12947986 | 12947986 |
| chr_4 | g17252 | g17252.t1 | isoform | g17252.t1 | 12948180 | 12950418 |
| chr_4 | g17252 | g17252.t1 | exon | g17252.t1.exon1 | 12948180 | 12949072 |
| chr_4 | g17252 | g17252.t1 | cds | g17252.t1.CDS1 | 12948180 | 12949072 |
| chr_4 | g17252 | g17252.t1 | exon | g17252.t1.exon2 | 12949128 | 12949430 |
| chr_4 | g17252 | g17252.t1 | cds | g17252.t1.CDS2 | 12949128 | 12949430 |
| chr_4 | g17252 | g17252.t1 | exon | g17252.t1.exon3 | 12949487 | 12950086 |
| chr_4 | g17252 | g17252.t1 | cds | g17252.t1.CDS3 | 12949487 | 12950086 |
| chr_4 | g17252 | g17252.t1 | exon | g17252.t1.exon4 | 12950196 | 12950418 |
| chr_4 | g17252 | g17252.t1 | cds | g17252.t1.CDS4 | 12950196 | 12950418 |
| chr_4 | g17252 | g17252.t1 | TSS | g17252.t1 | 12950974 | 12950974 |
Sequences
>g17252.t1 Gene=g17252 Length=2019
ATGATTAAATTTTGGCTTTTTTGGATATTTTTAATTTTTACAATTCCTTTTGTAATCTCA
AATGAAGTCATTGTTACAATTGCCTTTAATGATGACTATAATGGTGTTAAAGAAAGTTCA
CCAGGTGAATTTCCATTTCATGCTGCAATATATAGAAGTGACCACAGAGGTCTTAATTAT
ACATGCGGTGCATCATTGATAACTGAATACAATCTTTTAACGGCTGCTCATTGTTTTGAA
TACAGAGGTTTTTTCATAGAAAGTTATTCTGCAGTTCTTGGAGTTTATGATTTGACCGAC
TTAGCAGAAGAATCAAGAGTTTACAGAAAATTTTCATCAATTGATATTCATGATGAATTT
CAACTTATATCAAATGATGCTCTTAGCAATGCTGACATAGCTGTTGTGACAATGAACAAA
AAAGTTAAATTTTCTGATTTCATAAAACCTGTCAAACTTCCATCACCATACATGCAAATA
ATTGGTCTTATTGGGACAATTTTCGGTTATGGTCAAACATCCTCAGGTTCACAACATACA
AACAAACCACAAAAAGGTGAACTAAAAATTATTAGCAGTCATGAATGTCAGAAGAAAGGT
CAAGATTATCCTGGGATTTTATCATCAAGAAGTTTCTGTGCTGGTGATAGTTTTGCAACT
CCTTGTCAAGGCGATTCTGGTGGTGGATTAGTTATAAAAAATGATGAAGGTGAATTTGAA
ATTATTGGAATTGTTTCACATAGACTTTCTAAAAATGAATGCAGTCCAAATGATTATGCA
GTTTTTGTTGATGTCTCAAGATTTACAGTTTGGATTCAATCAAAACTTCATCCTTGCTTT
TCATCACCTTGTGGACAAAATGGAAAATGCATAAATATTGATGACAGAGTCACTTGTGTT
TGTCACGATGATTTTATTGGAATTTATCCAAATTGTGAAAAAATTGAATGTTGGAAAGTT
ACAGATTGTGCTGATGATCGAGCTTGTCTAAATAATAAATGTTTTGATTTATGTGCTTTA
GAAAATTGTGGACCAAATTCTGTTTGCCTCATGGAAGACCATAAGAAAATTTGTGAATGT
CAATTGAACTTTAAACTGGAAAAAAATCAATGCACATTAAAATCAAATCTTCTCGATGGC
AGCTTGTTTAGTTTTAACATACCAAAAAATTGTAATTTTGGAGAATGTGGATCTTTAAAT
TATCTCACTTTTTTCGTCTCTTCAAATACGAAAATTTTAATAACTGATGCAGAAGTAATG
TCAAAACATCAAAATGATAAAATTGATGGTTTTCGAATTTATTGTGATGATTGCGATAAA
AAGGGAAAACAAGACATTTTTCATCTTCCAATCAATGTCATTAAAATTTTTCCAAATTTA
CTTGCTTATTTTGCACATCATACAAAAGTTAAAAAATTAACTCGTGAAAATTTCAAGGAA
ATGAAGAAATTAGATCAATTGAATCTTCATACTAATGAAATTGAAGAAATTGATGATGAT
GCATTTGATGATTTGATAAAACTTGAATATCTTAATCTGTCTGAAAATAATTTGAAAATT
TTGAATCCAAGGATTTTTTTGGCTTTGAGAAATTTGAAATATTTTCATGTGTCTAAAAAT
CAACTGACTTCATTAACTGCTCATGTTTTTGAAAATTTGGTTAATCTTAAACATTTTATA
CTCAACGAGAATTACATAAAATCTTTTCCATCTGGAATTTTTGATAGATTAACTAACCTT
TCAATCATTGATACTCATAACAATCAATTTACAACTTTTGATGAAAATATTTTCAAAAAT
AATTTCAAGCTTGAAGGAATTTATTTATGGAACAATAAAATAACAAAATTGAGCTACAAA
TTATTTGAAAATAAAGAATTTTTAAAACGAGTTGATTTAAGTAGAAATATTTGCATTAAT
TTACTTTTGTATGTCAATAATGATAAAGGTGAACACATCAATTTACCAGAAGAAAAAAGA
AATGAATTGAAAAATTTACTTATCGGAAAATGTTCATAA
>g17252.t1 Gene=g17252 Length=672
MIKFWLFWIFLIFTIPFVISNEVIVTIAFNDDYNGVKESSPGEFPFHAAIYRSDHRGLNY
TCGASLITEYNLLTAAHCFEYRGFFIESYSAVLGVYDLTDLAEESRVYRKFSSIDIHDEF
QLISNDALSNADIAVVTMNKKVKFSDFIKPVKLPSPYMQIIGLIGTIFGYGQTSSGSQHT
NKPQKGELKIISSHECQKKGQDYPGILSSRSFCAGDSFATPCQGDSGGGLVIKNDEGEFE
IIGIVSHRLSKNECSPNDYAVFVDVSRFTVWIQSKLHPCFSSPCGQNGKCINIDDRVTCV
CHDDFIGIYPNCEKIECWKVTDCADDRACLNNKCFDLCALENCGPNSVCLMEDHKKICEC
QLNFKLEKNQCTLKSNLLDGSLFSFNIPKNCNFGECGSLNYLTFFVSSNTKILITDAEVM
SKHQNDKIDGFRIYCDDCDKKGKQDIFHLPINVIKIFPNLLAYFAHHTKVKKLTRENFKE
MKKLDQLNLHTNEIEEIDDDAFDDLIKLEYLNLSENNLKILNPRIFLALRNLKYFHVSKN
QLTSLTAHVFENLVNLKHFILNENYIKSFPSGIFDRLTNLSIIDTHNNQFTTFDENIFKN
NFKLEGIYLWNNKITKLSYKLFENKEFLKRVDLSRNICINLLLYVNNDKGEHINLPEEKR
NELKNLLIGKCS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g17252.t1 | CDD | cd00190 | Tryp_SPc | 35 | 275 | 9.56265E-54 |
| 23 | g17252.t1 | CDD | cd00053 | EGF | 281 | 313 | 0.0014144 |
| 15 | g17252.t1 | Gene3D | G3DSA:2.40.10.10 | - | 37 | 266 | 2.1E-51 |
| 16 | g17252.t1 | Gene3D | G3DSA:2.40.10.10 | - | 45 | 271 | 2.1E-51 |
| 12 | g17252.t1 | Gene3D | G3DSA:2.10.25.10 | Laminin | 272 | 314 | 1.8E-6 |
| 14 | g17252.t1 | Gene3D | G3DSA:3.80.10.10 | Ribonuclease Inhibitor | 434 | 540 | 4.9E-17 |
| 13 | g17252.t1 | Gene3D | G3DSA:3.80.10.10 | Ribonuclease Inhibitor | 541 | 662 | 1.0E-19 |
| 3 | g17252.t1 | PANTHER | PTHR24260 | - | 37 | 276 | 9.9E-48 |
| 4 | g17252.t1 | PANTHER | PTHR24260:SF129 | MODULAR SERINE PROTEASE | 37 | 276 | 9.9E-48 |
| 7 | g17252.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 63 | 78 | 4.6E-7 |
| 5 | g17252.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 128 | 142 | 4.6E-7 |
| 6 | g17252.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 219 | 231 | 4.6E-7 |
| 2 | g17252.t1 | Pfam | PF00089 | Trypsin | 35 | 272 | 3.3E-39 |
| 1 | g17252.t1 | Pfam | PF13855 | Leucine rich repeat | 483 | 542 | 1.7E-13 |
| 18 | g17252.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 19 | g17252.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 20 | g17252.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 21 | g17252.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 17 | g17252.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 672 | - |
| 34 | g17252.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 73 | 78 | - |
| 41 | g17252.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 18 | 277 | 23.491 |
| 42 | g17252.t1 | ProSiteProfiles | PS50026 | EGF-like domain profile. | 275 | 313 | 13.245 |
| 36 | g17252.t1 | ProSiteProfiles | PS51450 | Leucine-rich repeat profile. | 483 | 504 | 7.011 |
| 40 | g17252.t1 | ProSiteProfiles | PS51450 | Leucine-rich repeat profile. | 507 | 528 | 7.412 |
| 39 | g17252.t1 | ProSiteProfiles | PS51450 | Leucine-rich repeat profile. | 531 | 552 | 5.756 |
| 38 | g17252.t1 | ProSiteProfiles | PS51450 | Leucine-rich repeat profile. | 555 | 576 | 5.879 |
| 37 | g17252.t1 | ProSiteProfiles | PS51450 | Leucine-rich repeat profile. | 579 | 600 | 4.793 |
| 35 | g17252.t1 | ProSiteProfiles | PS51450 | Leucine-rich repeat profile. | 603 | 624 | 5.987 |
| 25 | g17252.t1 | SMART | SM00020 | trypsin_2 | 33 | 272 | 2.3E-45 |
| 33 | g17252.t1 | SMART | SM00181 | egf_5 | 278 | 313 | 0.0014 |
| 32 | g17252.t1 | SMART | SM00181 | egf_5 | 337 | 372 | 22.0 |
| 29 | g17252.t1 | SMART | SM00369 | LRR_typ_2 | 481 | 504 | 0.75 |
| 28 | g17252.t1 | SMART | SM00369 | LRR_typ_2 | 505 | 528 | 0.36 |
| 27 | g17252.t1 | SMART | SM00369 | LRR_typ_2 | 529 | 552 | 0.14 |
| 31 | g17252.t1 | SMART | SM00369 | LRR_typ_2 | 553 | 576 | 0.42 |
| 30 | g17252.t1 | SMART | SM00369 | LRR_typ_2 | 577 | 600 | 0.67 |
| 26 | g17252.t1 | SMART | SM00369 | LRR_typ_2 | 603 | 624 | 210.0 |
| 9 | g17252.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 28 | 276 | 7.07E-55 |
| 10 | g17252.t1 | SUPERFAMILY | SSF57196 | EGF/Laminin | 273 | 309 | 5.08E-6 |
| 8 | g17252.t1 | SUPERFAMILY | SSF52058 | L domain-like | 445 | 641 | 5.35E-36 |
| 11 | g17252.t1 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 20 | - |
| 24 | g17252.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
