General information for g17446.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Transmembrane protease serine 11A.
Parent gene
- The parent gene g17446 is located on chr_4: 13554148..13556604 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17446 | g17446.t1 | isoform | g17446.t1 | 13552229 | 13554148 |
| chr_4 | g17446 | g17446.t1 | exon | g17446.t1.exon1 | 13552229 | 13553187 |
| chr_4 | g17446 | g17446.t1 | cds | g17446.t1.CDS1 | 13552229 | 13553187 |
| chr_4 | g17446 | g17446.t1 | exon | g17446.t1.exon2 | 13553245 | 13553844 |
| chr_4 | g17446 | g17446.t1 | cds | g17446.t1.CDS2 | 13553245 | 13553844 |
| chr_4 | g17446 | g17446.t1 | exon | g17446.t1.exon3 | 13553932 | 13554148 |
| chr_4 | g17446 | g17446.t1 | cds | g17446.t1.CDS3 | 13553932 | 13554148 |
| chr_4 | g17446 | g17446.t1 | TSS | g17446.t1 | NA | NA |
| chr_4 | g17446 | g17446.t1 | TTS | g17446.t1 | NA | NA |
Sequences
>g17446.t1 Gene=g17446 Length=1776
ATGTTGACTTATTTGTTTATTTGCACAATAATCTTTTCGTTCTCTACACAATCAGACGAT
TGTGGACGTGTTTTTGTTTCACAATCAACAAATATGATTGTCGGCAAAGGAGCGAAAGAT
GTTTATAAAGGAGAATGGCCATGGCATGTTCCAGTGTTTTATGATAATAGCTATATTTGT
GGTTCATCTTTAATTTCTGAAAAGCATTTGTTGACTGCTGCTCATTGTTTAGCTCATGAT
GAAAATGTTGAAAATTATTTCGTCTTACCTGGAAAATTTAACCTAACAAATGATGAAGAA
TCAAATTGGATAAATCGAACGATTTCAAAAATGATAATTCATGACGAATATAATCCAGAT
TCAGCAGCATTTCGAAGCAATTCTGACATTGCTATATTAGTAATGAGCTCAATGGTAATT
TTTAATGATTTTATTTTCCCAATTTGTCTTCCTAATCCTGATAATAAAGTCAATGACTTT
TATGGAACTGTTGTTGGACATGGTTTTAATAATAATGAAACTGAATATGAAAACACACCA
AAATCTGCAGTTTTACACACAATTGGTTCAATGCAGTGCATTTTTAAGCACAAAAAATAT
TCAATTACTGTATCAGAAAGAAGTTTTTGTGCCATCAATCCAATTGCAAGTCCATGTCGA
GGCGATTCTGGTGGTGGATTTTTTGTAAGAAATAATGAAACTGAACAATTTACAATTTAT
GGAGTTGTGTCACAGAAAAATAATTTGAAAAAATGTTCGATTAATGATTTTACTGTTTTT
GTTGATGTTACAAAATTTGCTTATTGGATTCAATCAAAAATTTTAACGAAAACTCTTAAC
TTTTGTGAAATTCAGCCATCACCTTGTGGTCCAAATTCAAAATGCATAAATTTCAATGAG
AACGGCTTCTGTTCTTGTGAAGAAAATTTTCTTGGTTTGCCACCAAATTGCAAGAAACCT
GAATGTATTTCAAATTATGAATGCCCAACTGATAAAACTTGTGTCAAAAATGAATGCATT
GATTTATGTCAATTTAGTAATTGTGGTGAAAACTCAGAATGTATCGTTCAAAATCATAAA
ATGACATGTAAAAATCAAAAAGAGGATTTTGATGAAAAATTGAGGCAATTAGAAGAAAAA
TTAAAAGAATTATCACAAAATGAAAGAAATATTGATGATTTTTGGGATTCAGATGATGAA
ATCTCAATAAGTGAGAGTTTAAAAATGTTGGAAGGTTTACTAAATGAAATAAAAACCCTG
GAAGAAATTTTATCAAAAACTGATGATATATTGAATAGTTCAATAGAAATAACTACAATG
GAAAATACAAAAAATATTCAAGAGTTAAAAGACACAAACATTGAAGAAACATCACAGAAT
GAAATACAAATAGAAATCAATCCAACAACAAAAGCAAAATTTATCTGTCATCTTCCAATT
AGAGGAGGAAATTGTATAGTTTATGAAAAGAAATTCTTTTTCAACAAATTTTATGGAATT
TGTGCACCATTTTTTGGTTGTGGCCCAAATCAAAACAGTTTTGAAACTCAAGAAGAATGT
GAAGAACTTTGCATGAATACTGTTAACTTTTGTGATTTAGCACCACGAAGAGGAAGCTGT
AATGACAAAAATACTAAATGGTTCTTTAATTCTACAGAAATGAAGTGTCAAAAGTTTAGT
TATTCAGGATGTAATGGAAATAGAAATAATTTTGATGATGAAAATTCTTGCATGAAAGCT
TGCTCTCATAAAGTTTTAGAATTTAATTTTAAATAA
>g17446.t1 Gene=g17446 Length=591
MLTYLFICTIIFSFSTQSDDCGRVFVSQSTNMIVGKGAKDVYKGEWPWHVPVFYDNSYIC
GSSLISEKHLLTAAHCLAHDENVENYFVLPGKFNLTNDEESNWINRTISKMIIHDEYNPD
SAAFRSNSDIAILVMSSMVIFNDFIFPICLPNPDNKVNDFYGTVVGHGFNNNETEYENTP
KSAVLHTIGSMQCIFKHKKYSITVSERSFCAINPIASPCRGDSGGGFFVRNNETEQFTIY
GVVSQKNNLKKCSINDFTVFVDVTKFAYWIQSKILTKTLNFCEIQPSPCGPNSKCINFNE
NGFCSCEENFLGLPPNCKKPECISNYECPTDKTCVKNECIDLCQFSNCGENSECIVQNHK
MTCKNQKEDFDEKLRQLEEKLKELSQNERNIDDFWDSDDEISISESLKMLEGLLNEIKTL
EEILSKTDDILNSSIEITTMENTKNIQELKDTNIEETSQNEIQIEINPTTKAKFICHLPI
RGGNCIVYEKKFFFNKFYGICAPFFGCGPNQNSFETQEECEELCMNTVNFCDLAPRRGSC
NDKNTKWFFNSTEMKCQKFSYSGCNGNRNNFDDENSCMKACSHKVLEFNFK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 26 | g17446.t1 | CDD | cd00190 | Tryp_SPc | 33 | 273 | 1.34155E-41 |
| 27 | g17446.t1 | CDD | cd00109 | KU | 475 | 525 | 4.67997E-12 |
| 28 | g17446.t1 | CDD | cd00109 | KU | 529 | 581 | 9.6613E-17 |
| 19 | g17446.t1 | Coils | Coil | Coil | 360 | 394 | - |
| 20 | g17446.t1 | Coils | Coil | Coil | 403 | 430 | - |
| 18 | g17446.t1 | Gene3D | G3DSA:2.40.10.10 | - | 27 | 275 | 8.1E-50 |
| 17 | g17446.t1 | Gene3D | G3DSA:4.10.410.10 | Factor Xa Inhibitor | 451 | 526 | 1.5E-12 |
| 16 | g17446.t1 | Gene3D | G3DSA:4.10.410.10 | Factor Xa Inhibitor | 527 | 589 | 8.4E-18 |
| 4 | g17446.t1 | PANTHER | PTHR24260 | - | 6 | 299 | 6.7E-50 |
| 5 | g17446.t1 | PANTHER | PTHR24260:SF110 | GH19262P | 6 | 299 | 6.7E-50 |
| 8 | g17446.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 61 | 76 | 1.6E-7 |
| 7 | g17446.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 125 | 139 | 1.6E-7 |
| 9 | g17446.t1 | PRINTS | PR00759 | Basic protease (Kunitz-type) inhibitor family signature | 207 | 221 | 6.6E-7 |
| 6 | g17446.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 216 | 228 | 1.6E-7 |
| 11 | g17446.t1 | PRINTS | PR00759 | Basic protease (Kunitz-type) inhibitor family signature | 556 | 566 | 6.6E-7 |
| 10 | g17446.t1 | PRINTS | PR00759 | Basic protease (Kunitz-type) inhibitor family signature | 566 | 581 | 6.6E-7 |
| 1 | g17446.t1 | Pfam | PF00089 | Trypsin | 39 | 270 | 2.6E-37 |
| 3 | g17446.t1 | Pfam | PF00014 | Kunitz/Bovine pancreatic trypsin inhibitor domain | 475 | 525 | 5.3E-10 |
| 2 | g17446.t1 | Pfam | PF00014 | Kunitz/Bovine pancreatic trypsin inhibitor domain | 530 | 581 | 4.8E-16 |
| 22 | g17446.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 23 | g17446.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 24 | g17446.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 13 | - |
| 25 | g17446.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 21 | g17446.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 591 | - |
| 33 | g17446.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 71 | 76 | - |
| 32 | g17446.t1 | ProSitePatterns | PS00280 | Pancreatic trypsin inhibitor (Kunitz) family signature. | 559 | 577 | - |
| 36 | g17446.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 33 | 275 | 22.445 |
| 37 | g17446.t1 | ProSiteProfiles | PS50026 | EGF-like domain profile. | 278 | 318 | 10.298 |
| 34 | g17446.t1 | ProSiteProfiles | PS50279 | Pancreatic trypsin inhibitor (Kunitz) family profile. | 476 | 524 | 11.105 |
| 35 | g17446.t1 | ProSiteProfiles | PS50279 | Pancreatic trypsin inhibitor (Kunitz) family profile. | 531 | 581 | 14.581 |
| 29 | g17446.t1 | SMART | SM00020 | trypsin_2 | 32 | 270 | 3.6E-31 |
| 30 | g17446.t1 | SMART | SM00131 | KU_2 | 474 | 525 | 3.0E-9 |
| 31 | g17446.t1 | SMART | SM00131 | KU_2 | 529 | 582 | 1.3E-18 |
| 14 | g17446.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 13 | 274 | 7.29E-56 |
| 13 | g17446.t1 | SUPERFAMILY | SSF57362 | BPTI-like | 472 | 528 | 4.11E-12 |
| 12 | g17446.t1 | SUPERFAMILY | SSF57362 | BPTI-like | 529 | 584 | 1.56E-15 |
| 15 | g17446.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004867 | serine-type endopeptidase inhibitor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
