General information for g17450.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glutathione S-transferase E14.
Parent gene
- The parent gene g17450 is located on chr_4: 13573365..13574377 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17450 | g17450.t1 | isoform | g17450.t1 | 13570081 | 13573365 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon1 | 13570081 | 13570178 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS1 | 13570081 | 13570178 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon2 | 13570237 | 13570486 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS2 | 13570237 | 13570486 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon3 | 13570706 | 13571003 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS3 | 13570706 | 13571003 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon4 | 13571190 | 13571336 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS4 | 13571190 | 13571336 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon5 | 13571551 | 13572325 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS5 | 13571551 | 13572325 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon6 | 13572453 | 13572568 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS6 | 13572453 | 13572568 |
| chr_4 | g17450 | g17450.t1 | exon | g17450.t1.exon7 | 13573196 | 13573365 |
| chr_4 | g17450 | g17450.t1 | cds | g17450.t1.CDS7 | 13573196 | 13573365 |
| chr_4 | g17450 | g17450.t1 | TTS | g17450.t1 | 13573549 | 13573549 |
| chr_4 | g17450 | g17450.t1 | TSS | g17450.t1 | NA | NA |
Sequences
>g17450.t1 Gene=g17450 Length=1854
ATGTCAAACAAACTAGTACTTTACTATGTCTCATCGAGTCCATCATGTCGTGCTGCTTAT
TTAGTGGCACGTGAACTTGGTCTCGATGTTGAACTTAAGTACATAAATTTATTTAGAGAA
GACAATTTGTCACCTGAATATTTAGCAATAAATCCATCAGGAACCGTTCCTTCTTTAGTT
GATGGTGATGTTACTGTTTGGGATAGCCATGCAATTATGATTTATTTGACTGAAAAATTT
GATAATAATTCTCAACTTTATCCAAGAGATTTTTTTAAACGAACTTTGATTAATGAACGA
TTGTTTTTTGAAGCAAGTTATTTATTTGCTAGACTTTTTGAAATTTGTGATCCACTTTGT
GATGGACGTGAAAAAATAATTTCGCAAGATAAAATCGATCGAGTCATTCGTGGTTATAGA
AGTGTTGAAGATTTCTTTTTTGATGATAATCAATTCATTGCTGCTCCAATAATGACTTTG
GCTGATTTTAGTATGTGGACAACTCTCATAACTCTCAATTATCTCGTGCCTATTAATGAA
GAAAAATTTCCTAAATTGACAAAATATTTGAAAATGTTGGAAAAACATGAAGGATATGAA
ATTAATCGAGACGGTGCTGAACGACATGCAAATTATGTTCTGAAATGTCTCCATGGAATG
GAATACAATGGAAAAATTGCTGTTGAAATTTTCAGAGAATCAAAAAATTATTCTCATATC
ATTAATGAATGGGATCACATTACAGCTAAAGAATTTGAAGCTAAAAAAGATGAAAATAAT
GAAGAAGAAAAAGTTATAAACAAAATGTCAAACTTATGTCGTGTGTGTGTAACAGAAAGC
TCTGACACAGTTGAGCTCAATGAATATCGTGATGGCTTGCCAATTTCAGTCATCGTAATG
ATAATTCTGCCTGTAAAGATCTATGAAGGCGAAGAATTGAAACTTCCAAAGAAAATTTGT
GGAAAGTGCCTCGAAATTATTTTAAACGCTTACAATTTGCGTTCTGTGAGTTTAAATAGT
GAACAATTTTTAAAATCAAATGAAATTGACATCAAAAATGAAGTTATTGAAAAACCAATT
GAGATTACAGAAATAAAAGAAAAATTTACAAAATTTTTTGATGAGCCAGAAAAAGAACAA
AATGAAGAAATTTTTTCAGAACAAATTTCAATTGATTTGTATATGAAAAATAAAAAACGT
TCAACATGTTGGCAATATTTTGGTCGATTACTTAATGAATATCGAGATGAAATCGATACT
GACTTTAATTACTGCTCACTATGTTTAAAAGAGAAGAAAATTACAAAATACAAATGTTCA
ACAGCAACTTCTGCAATGATTCAACATTTACAAGTAATTCATAGAATTGGCGGACAAGAA
GCTGGCAGTAAAATGATGATAAATAAAGTTCAGGAAGAATACAAACAAAAGACAATAATA
GCTGATAAAAATTGCTCGGAAGCTTCAGAAACACAATGCAAAATTTGTGGAAAAAATTTT
AGTAACCATCATACTTTGAATAAGCATTTACAAACTCATTCTGGACCATCTTTTTATTCA
TGTGATAGTTGTCCAGCAAGATTCATTTACCTTGAAAATCTTCAACGGCATCAAAAAGTT
CATGACACAAATCATTTTTCACAATACATTTGTGACATTTGTGGTAATGATTATGCTTCT
AAAGATGATCTTCATAGACATTTACAAACGAACCACGTTGGTAATACATCCTATACTTGT
AAATGTTGTTCAGCTTCATTCAGACTTAAAATACAATTAAAAAATCATTACAAATCTCAT
CACAAAAATAGTTATTACACAGAGGCAGAAGGCAGTGGAATAAGTAATTTGTAG
>g17450.t1 Gene=g17450 Length=617
MSNKLVLYYVSSSPSCRAAYLVARELGLDVELKYINLFREDNLSPEYLAINPSGTVPSLV
DGDVTVWDSHAIMIYLTEKFDNNSQLYPRDFFKRTLINERLFFEASYLFARLFEICDPLC
DGREKIISQDKIDRVIRGYRSVEDFFFDDNQFIAAPIMTLADFSMWTTLITLNYLVPINE
EKFPKLTKYLKMLEKHEGYEINRDGAERHANYVLKCLHGMEYNGKIAVEIFRESKNYSHI
INEWDHITAKEFEAKKDENNEEEKVINKMSNLCRVCVTESSDTVELNEYRDGLPISVIVM
IILPVKIYEGEELKLPKKICGKCLEIILNAYNLRSVSLNSEQFLKSNEIDIKNEVIEKPI
EITEIKEKFTKFFDEPEKEQNEEIFSEQISIDLYMKNKKRSTCWQYFGRLLNEYRDEIDT
DFNYCSLCLKEKKITKYKCSTATSAMIQHLQVIHRIGGQEAGSKMMINKVQEEYKQKTII
ADKNCSEASETQCKICGKNFSNHHTLNKHLQTHSGPSFYSCDSCPARFIYLENLQRHQKV
HDTNHFSQYICDICGNDYASKDDLHRHLQTNHVGNTSYTCKCCSASFRLKIQLKNHYKSH
HKNSYYTEAEGSGISNL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g17450.t1 | CDD | cd03045 | GST_N_Delta_Epsilon | 5 | 78 | 1.60972E-31 |
| 21 | g17450.t1 | CDD | cd03177 | GST_C_Delta_Epsilon | 93 | 207 | 5.65764E-22 |
| 15 | g17450.t1 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 82 | 2.5E-25 |
| 14 | g17450.t1 | Gene3D | G3DSA:1.20.1050.10 | - | 83 | 221 | 1.1E-30 |
| 12 | g17450.t1 | Gene3D | G3DSA:3.30.160.60 | Classic Zinc Finger | 464 | 545 | 1.4E-13 |
| 13 | g17450.t1 | Gene3D | G3DSA:3.30.160.60 | Classic Zinc Finger | 546 | 613 | 4.0E-10 |
| 5 | g17450.t1 | PANTHER | PTHR43969:SF4 | FI01423P-RELATED | 3 | 214 | 1.8E-57 |
| 6 | g17450.t1 | PANTHER | PTHR43969 | GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED | 3 | 214 | 1.8E-57 |
| 2 | g17450.t1 | Pfam | PF13417 | Glutathione S-transferase, N-terminal domain | 7 | 83 | 4.6E-15 |
| 1 | g17450.t1 | Pfam | PF07776 | Zinc-finger associated domain (zf-AD) | 272 | 345 | 1.0E-7 |
| 3 | g17450.t1 | Pfam | PF00096 | Zinc finger, C2H2 type | 492 | 513 | 0.0014 |
| 4 | g17450.t1 | Pfam | PF00096 | Zinc finger, C2H2 type | 519 | 541 | 0.008 |
| 17 | g17450.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 18 | g17450.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 19 | g17450.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 13 | - |
| 20 | g17450.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 16 | g17450.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 617 | - |
| 31 | g17450.t1 | ProSitePatterns | PS00028 | Zinc finger C2H2 type domain signature. | 493 | 513 | - |
| 32 | g17450.t1 | ProSitePatterns | PS00028 | Zinc finger C2H2 type domain signature. | 521 | 541 | - |
| 33 | g17450.t1 | ProSitePatterns | PS00028 | Zinc finger C2H2 type domain signature. | 551 | 572 | - |
| 35 | g17450.t1 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 3 | 84 | 22.12 |
| 34 | g17450.t1 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 90 | 213 | 11.02 |
| 40 | g17450.t1 | ProSiteProfiles | PS50808 | Zinc finger BED-type profile. | 398 | 461 | 9.979 |
| 36 | g17450.t1 | ProSiteProfiles | PS50157 | Zinc finger C2H2 type domain profile. | 491 | 518 | 12.217 |
| 39 | g17450.t1 | ProSiteProfiles | PS50157 | Zinc finger C2H2 type domain profile. | 519 | 541 | 10.554 |
| 37 | g17450.t1 | ProSiteProfiles | PS50157 | Zinc finger C2H2 type domain profile. | 549 | 577 | 10.928 |
| 38 | g17450.t1 | ProSiteProfiles | PS50157 | Zinc finger C2H2 type domain profile. | 578 | 600 | 9.806 |
| 41 | g17450.t1 | SFLD | SFLDG00358 | Main (cytGST) | 4 | 215 | 0.0 |
| 42 | g17450.t1 | SFLD | SFLDG01153 | Main.4: Theta-like | 4 | 215 | 0.0 |
| 23 | g17450.t1 | SMART | SM00868 | zf_AD_2 | 272 | 347 | 3.0E-4 |
| 25 | g17450.t1 | SMART | SM00614 | bed5 | 398 | 457 | 0.002 |
| 26 | g17450.t1 | SMART | SM00355 | c2h2final6 | 423 | 454 | 270.0 |
| 24 | g17450.t1 | SMART | SM00614 | bed5 | 465 | 516 | 1.1 |
| 27 | g17450.t1 | SMART | SM00355 | c2h2final6 | 491 | 513 | 0.01 |
| 30 | g17450.t1 | SMART | SM00355 | c2h2final6 | 519 | 541 | 0.026 |
| 29 | g17450.t1 | SMART | SM00355 | c2h2final6 | 549 | 572 | 0.019 |
| 28 | g17450.t1 | SMART | SM00355 | c2h2final6 | 578 | 600 | 0.17 |
| 7 | g17450.t1 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 4 | 88 | 8.42E-26 |
| 8 | g17450.t1 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 83 | 200 | 9.75E-14 |
| 9 | g17450.t1 | SUPERFAMILY | SSF57716 | Glucocorticoid receptor-like (DNA-binding domain) | 271 | 345 | 7.13E-7 |
| 10 | g17450.t1 | SUPERFAMILY | SSF57667 | beta-beta-alpha zinc fingers | 492 | 541 | 6.18E-12 |
| 11 | g17450.t1 | SUPERFAMILY | SSF57667 | beta-beta-alpha zinc fingers | 528 | 587 | 9.42E-9 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005634 | nucleus | CC |
| GO:0003677 | DNA binding | MF |
| GO:0005515 | protein binding | MF |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K00799 (glutathione S-transferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
