General information for g17821.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Tyrosine-protein kinase Shark.
Parent gene
- The parent gene g17821 is located on HiC_scaffold_384: 114..3301 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| HiC_scaffold_384 | g17821 | g17821.t2 | isoform | g17821.t2 | 114 | 1743 |
| HiC_scaffold_384 | g17821 | g17821.t2 | exon | g17821.t2.exon1 | 114 | 1743 |
| HiC_scaffold_384 | g17821 | g17821.t2 | cds | g17821.t2.CDS1 | 491 | 1489 |
| HiC_scaffold_384 | g17821 | g17821.t2 | TSS | g17821.t2 | 2516 | 2516 |
| HiC_scaffold_384 | g17821 | g17821.t2 | TTS | g17821.t2 | 18774 | 18774 |
Sequences
>g17821.t2 Gene=g17821 Length=1630
TTGTTGAATATTTGGAAAAATATGTGCCAGTTTGTAATACATTTAGCCATAAGTGGCATC
ATGGAACATTAGATAGAGAAGGGGCAAGAACTTTGTTATTGAAGAAAAGAAATGAACTTT
ATGAAAAATATCGTGAAGAATATGCATTGGAAGAGAATGTCTATGTTAATGATAATAAAG
AAATTAATGATTTAATTTGTGGATTGTTCTTGGTAAGAACAAGTGAAAGAAATGGTGGAT
TAGATGTCATAACTATGCTTCATGATGACGATGACCTAAATATTAGAAATTATGTAATTC
ATAAATCTGACAATTTAAAATATTTTTTATACGATGATGGACCATTTTTCACTTCATTAG
AACATCTTATAAGCTATTACATGACTCATGCTGATGGGCTTCCGGCTAAATTAACACAAT
TTGTGTCTCCAATCGTTCGTCCACCAATTCCAACTTTACAGAAAAAGTCAAAATCTGTGA
TGCAATCTCCAGTTGTTAGTACAAATTCAATATCATCACCTGAAAGTGCTAACAATTCAA
TGACAATAGAGTCTCAAACAAAAGCAAAATCATCAGTTTTCACTTTATTCAAGAAAAAAA
AGCATTCCCTTCCAACTTCACTCTCTAAAGAAGACAAACCATCAGTTGATTTGTCAATGG
ATTCTTTCAATAAATCATTGTCATTTTCAACGAATTTTCTTACAAATTATGGCGTTCCTC
CGACATCAAAGAGGCCATCAACACAAGAAGATCAATTTACAGAAAGCGATCAATTTTTGA
TCAACAATCAGACAGTTCAAGACTCAAAACCACAAGAAATGATTTATTTCATGGATCCAC
CAAAAAGGAATAAAATAGAATGGAATCAACTTGATCCGGAATTGTATGAAACGCAAAAGA
ACTTTTTAAAAGAAATTACTCGAAGTTCTGGTGCAAATTATTATGTCTCAAAAGATGATC
TACATTTTGACAATGAAATTGGTTCAGGAGAATTTGGAAATGTCTTAAGGGGAATTTTTA
AATTATCAAATGGCAAGAAAATTTGGGTTGCAATAAAAACTTTACATGAGAGGCATTATG
AAGAAAATCTTCCAGAATTTTTAAAAGAAGCTTCAGTTATGATAAAATTAGACAATTCCT
ATGTCGTAAAACTAATTGTTCAAGAGCTCTGTAGTTTTGGATCACTCGCTGAATATTTAG
TAGAAAACAAAGATGATATCGATTATAAATTAATTGACTTGTGGGCGTCTTAAATTGCAC
AAGGTATGGAATATCTTGAATCGAAGAGATTTGTACATCGTGATCTTGCAGCTCGCAATA
TTTACTCGTCTCGAAGCAACATTGCAAAATATCTAACTTTGGTCTCTCACGTGCCATTGG
AATCGATAAAGATTTCTATCAATCAAGTACTGGTGGACGCTGGCCATTAAAATGGTATGC
GCCTGAATCTTTCTGTGGAAAATTTTCTCATTCAAGTGATGTGTGGAGTTTCGGTATAAC
TTTGTGGGAAATTTATTCAAAAAGTGATGTACCCTATGATGATTTGTCTGGAAGTGAAGT
CAATGAATTAATTGAGAAAGGTCAGCGTCTTGCAAGACCAGAAGGTTGTCCATCCAGAAG
ATGTGTATAG
>g17821.t2 Gene=g17821 Length=332
MLHDDDDLNIRNYVIHKSDNLKYFLYDDGPFFTSLEHLISYYMTHADGLPAKLTQFVSPI
VRPPIPTLQKKSKSVMQSPVVSTNSISSPESANNSMTIESQTKAKSSVFTLFKKKKHSLP
TSLSKEDKPSVDLSMDSFNKSLSFSTNFLTNYGVPPTSKRPSTQEDQFTESDQFLINNQT
VQDSKPQEMIYFMDPPKRNKIEWNQLDPELYETQKNFLKEITRSSGANYYVSKDDLHFDN
EIGSGEFGNVLRGIFKLSNGKKIWVAIKTLHERHYEENLPEFLKEASVMIKLDNSYVVKL
IVQELCSFGSLAEYLVENKDDIDYKLIDLWAS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g17821.t2 | Gene3D | G3DSA:3.30.505.10 | SHC Adaptor Protein | 1 | 58 | 5.4E-9 |
| 7 | g17821.t2 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 197 | 307 | 3.4E-18 |
| 2 | g17821.t2 | PANTHER | PTHR24418 | TYROSINE-PROTEIN KINASE | 16 | 331 | 1.2E-26 |
| 3 | g17821.t2 | PANTHER | PTHR24418:SF372 | TYROSINE-PROTEIN KINASE SHARK | 16 | 331 | 1.2E-26 |
| 1 | g17821.t2 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 236 | 323 | 7.8E-13 |
| 6 | g17821.t2 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 242 | 268 | - |
| 9 | g17821.t2 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 236 | 332 | 8.598 |
| 5 | g17821.t2 | SUPERFAMILY | SSF55550 | SH2 domain | 10 | 254 | 7.33E-9 |
| 4 | g17821.t2 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 221 | 325 | 6.53E-16 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
