General information for g1828.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein unc-13 homolog 4B.
Parent gene
- The parent gene g1828 is located on chr_3: 13218929..13219765 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1828 | g1828.t1 | TTS | g1828.t1 | 13216163 | 13216163 |
| chr_3 | g1828 | g1828.t1 | isoform | g1828.t1 | 13216251 | 13219765 |
| chr_3 | g1828 | g1828.t1 | exon | g1828.t1.exon1 | 13216251 | 13216384 |
| chr_3 | g1828 | g1828.t1 | cds | g1828.t1.CDS1 | 13216251 | 13216384 |
| chr_3 | g1828 | g1828.t1 | exon | g1828.t1.exon2 | 13216444 | 13218041 |
| chr_3 | g1828 | g1828.t1 | cds | g1828.t1.CDS2 | 13216444 | 13218041 |
| chr_3 | g1828 | g1828.t1 | exon | g1828.t1.exon3 | 13218111 | 13219174 |
| chr_3 | g1828 | g1828.t1 | cds | g1828.t1.CDS3 | 13218111 | 13219174 |
| chr_3 | g1828 | g1828.t1 | exon | g1828.t1.exon4 | 13219225 | 13219525 |
| chr_3 | g1828 | g1828.t1 | cds | g1828.t1.CDS4 | 13219225 | 13219525 |
| chr_3 | g1828 | g1828.t1 | exon | g1828.t1.exon5 | 13219635 | 13219765 |
| chr_3 | g1828 | g1828.t1 | cds | g1828.t1.CDS5 | 13219635 | 13219765 |
| chr_3 | g1828 | g1828.t1 | TSS | g1828.t1 | 13219895 | 13219895 |
Sequences
>g1828.t1 Gene=g1828 Length=3228
ATGGATAAAGTAAAAGGAAGTAGAAGCACAGAAATACTGCCAGACTTTCGAAAAAGAAAC
ATATCGGGTGTATTCTCATCTTGGCGACCAAAAAAAGAAGAGAATAAACAGAATGTCGAA
ACTAAAAAGAAATCATCTGGATCAAAGTCAATTTTTTCAACATTAATGTTAGCGAATGTT
GAAGATCGTCTATATTTGGATCCATCGGGTTCAATGGAATCAATGACATTCGAGGCACAA
AAGGAAAAATTAAAAAGTAAACTACATCGAGTTAAACTTGAAAAATCAAAAATACCGCAA
ATTATTGTCACTAATTCCAATAATCAAAAGCAACCAAACAATAATAAAAATGTTGTGGAA
GAAGAAGAAGAAGAGGAGGAAATTGAATTGCCGAAACCAGTTTCTTTCATTGTATTGAAT
GTAGATCCATATATTCTAGATATATATCAAGACATTCTGTATAATATTACACACCAACTA
CAGTCATGTGACGACAATGATGAACCAGAAATTCAGCCTGAGGTTGCACTTAATTACGTT
CGAGATGCATTTGGCGTTAATGAATGTAAACATTTAGAATTGATGGAGAAAATAAAGCAG
CGAGAAAAGCCAGAAGATTGCGTCAAAGCAGACAGCGGTGAAATAGACAGCAGTACTGCA
CCCTTTGCAGTGCTCTCTCCTAAAATTAAAAATTATGAAAGTTTAAATGCTCATTGTGAT
TTGATAAAAATTTTACTTAAACACGAATTGGAAACATCAAAAACACCTCATTATTATTGG
ATGGGAAAATTTAGTTTCCTAGCTTCAAAAATTTTATCACTTCATGCAGACTTTCAATTT
TTAGATGAGCTGCAAGTTTCTTTTGCTAAATGGATAGCATTTGTAGAAATTCATAGACAG
TACCCATTACATCTAAAAGTATTTGAAGATACACTTGATAAAATTGTAGACGTTTACAAA
GATGAAGAGACGTCAACAGCACTTTTACCCGCTAGGATGTCTACAACTCTCACTTTCATT
CCTGCATGCTTCGGATTCACTGGTGCAATTAAACCTGGACTTAAAATTGAAGAATTAAAA
AATCGTACGAGCGATCACTTGAAAATTAAAGATGATGAAGTAATCAAACAATTTTGGAAT
TCTAATAATCTACTTCATGAGTCTTTTTTGAAATTCATTCATGATCTTCATTATAAACAT
CATGATGAAGAGAATGTAATTTTTAAAGCAATAATTTTGAAAAATATGTTTGAGATTGTA
AATAGAATCAAGAAAATTTTTCTGCCAAAAGACATTGAACAACTTAATTTTGAAAAACTT
TTAAAAAATGCATTGAATGATGGCACTACAGAACATCTCAAAAGAAATATGAAACATAGA
GCCCTTAAGAAATCAAGAAATTGCAAAGTACGTCTCGATGAATTAATTCGATTACTTAAA
TTCAGCGAAGAGCACATGAAAAGCATTGTCAATGAGTATGCACATGTTTTTGAATCCTTC
TGTAAATTCTCATTCACAGAGTACATTTATTCTATCTATGATTCACAATTGGCAAAAATA
ATAAAACCGATTGTAATAGAAATTTGTAGCACCAAAGAAGGAAACATTGACAATTTTCAA
AAAGATGATCAAACGATTGCAACAAAATTGTTTGAATTATATCGTGAGATAAAAAAGTTT
TCTGATCAGGGTTTAGAATATTACTATGGAGAACATGATTTACAGATGAAAGATTATTCT
AGATGGTTCACTGCCGGAATTGATAAGTGGTGCAAAGTTTCACTTTTTAATGCTACATCT
AGAATCAATAAAGCTATTGAAGTTGATAATTTAAAGCCAGAATTAAAAGAATCAAAACGA
TCATCTTCAGCTATTGACATGATGCAAATTCTTTATTCTGTTAAGTCATTTTGGGAGAAA
CTACAATGGCCTGACGCAAATAAAGAAGCTGAAATTGCTAAGCATGTTGCTGGTGATTTA
TGCCGTTTTGCAATAGATTATTTTGACAAATTTAGCTCAAAAGTACAAAAATCTGAAGCT
ATGAATATTACGGGTATATTTAAAGTGCCATTAGAATTATCAATTGCTGTTGCCAATTTT
GAATACATCTCTGAACAAATTCAAACATTAATTAAAGGTATGACAGAGGGAAAAGTTGAA
GATACAAGTAGCATCGAAAAACTTATTAATAACATGCTGGTACATTTAGAAAACACTATC
AAACAATTGCTCGAAATAACTCTCAGTAAATCAATAGAAACAATGCAAAAATTGATGCTT
GAAGGTGCTCAGAATAATAAAGTTGGCGACGATATTGCTGACAGACTGATGACCTATATC
GAGGACATGCTTTCAACTCTTGTAGAAGATTTACCAAGAAAAAATTTTGAAGTCGCCAAA
TCTATTATTTGGCAACAAAATTTACAAATTTTGTCTGAAATAATTCAAGTCTCATTAACG
AGACAGCAACCGCCTGCATTTTTTACAAATCTTCGCAGAATCTTGTCCATATTAAAAGAA
ATATTTCATTTTTCTGAAGAAAATGCAGACTATGTAATGAAGAAGAAAGTTGAAAGAATT
GATTATTTACTTGAATGCTATGGCCTTAATACATCTCGTCTGATTCATCAATACTTTAAA
GAGCGTTATGAACTTCAACTTCAGATTAACAAACAACCATTTAACCCATACGGAGTTCTC
TCTGTCTATTGCTTTTTTGTGAAAAACACTTTAAAATTGGAAATATTAAATGCAAGAAAT
CTCATTCCAATTGGACCAAATAAGAAATGTGATTCGTTTATAAAAATTCAATTGATTCCA
GAAAAGTATTTTTCGACTTTTCCCAGTTATAAAACGAAAGTGCAACAAAATACTCACTTC
CCACTCTACGATGAAATTTTTGAATATGAATTAACTGATGAGCAACGAAAAATTGAGGAT
GCCATAATATTCTTCAATATCAAAGACAAATATCTTTTAGGTAGTAATGAATGCATTGCT
GAAGCATTTTTAGCATTTCGCGATATTCCAGAATATACTATAAAAGACAACTTAAAACAG
ATTCATTTGACATTGACACGACTACAATGCGATGAACTTGAGTCATTAAAAGCATTACAA
CATCGTGCTCAAACAGGGGATAAAGCGGCTAAAGATTTTCTCTCAAAACTTAGATCGCGA
ACAATAATCTCACCAAAAAAGATGAAACTTCCAGTGACACAATTATAA
>g1828.t1 Gene=g1828 Length=1075
MDKVKGSRSTEILPDFRKRNISGVFSSWRPKKEENKQNVETKKKSSGSKSIFSTLMLANV
EDRLYLDPSGSMESMTFEAQKEKLKSKLHRVKLEKSKIPQIIVTNSNNQKQPNNNKNVVE
EEEEEEEIELPKPVSFIVLNVDPYILDIYQDILYNITHQLQSCDDNDEPEIQPEVALNYV
RDAFGVNECKHLELMEKIKQREKPEDCVKADSGEIDSSTAPFAVLSPKIKNYESLNAHCD
LIKILLKHELETSKTPHYYWMGKFSFLASKILSLHADFQFLDELQVSFAKWIAFVEIHRQ
YPLHLKVFEDTLDKIVDVYKDEETSTALLPARMSTTLTFIPACFGFTGAIKPGLKIEELK
NRTSDHLKIKDDEVIKQFWNSNNLLHESFLKFIHDLHYKHHDEENVIFKAIILKNMFEIV
NRIKKIFLPKDIEQLNFEKLLKNALNDGTTEHLKRNMKHRALKKSRNCKVRLDELIRLLK
FSEEHMKSIVNEYAHVFESFCKFSFTEYIYSIYDSQLAKIIKPIVIEICSTKEGNIDNFQ
KDDQTIATKLFELYREIKKFSDQGLEYYYGEHDLQMKDYSRWFTAGIDKWCKVSLFNATS
RINKAIEVDNLKPELKESKRSSSAIDMMQILYSVKSFWEKLQWPDANKEAEIAKHVAGDL
CRFAIDYFDKFSSKVQKSEAMNITGIFKVPLELSIAVANFEYISEQIQTLIKGMTEGKVE
DTSSIEKLINNMLVHLENTIKQLLEITLSKSIETMQKLMLEGAQNNKVGDDIADRLMTYI
EDMLSTLVEDLPRKNFEVAKSIIWQQNLQILSEIIQVSLTRQQPPAFFTNLRRILSILKE
IFHFSEENADYVMKKKVERIDYLLECYGLNTSRLIHQYFKERYELQLQINKQPFNPYGVL
SVYCFFVKNTLKLEILNARNLIPIGPNKKCDSFIKIQLIPEKYFSTFPSYKTKVQQNTHF
PLYDEIFEYELTDEQRKIEDAIIFFNIKDKYLLGSNECIAEAFLAFRDIPEYTIKDNLKQ
IHLTLTRLQCDELESLKALQHRAQTGDKAAKDFLSKLRSRTIISPKKMKLPVTQL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g1828.t1 | Gene3D | G3DSA:1.10.357.50 | - | 574 | 751 | 4.4E-15 |
| 14 | g1828.t1 | Gene3D | G3DSA:2.60.40.150 | - | 883 | 1024 | 3.5E-16 |
| 12 | g1828.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 24 | 48 | - |
| 10 | g1828.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 29 | 43 | - |
| 11 | g1828.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 103 | 126 | - |
| 2 | g1828.t1 | PANTHER | PTHR45999:SF2 | PROTEIN UNC-13 HOMOLOG 4B | 27 | 205 | 4.6E-160 |
| 5 | g1828.t1 | PANTHER | PTHR45999 | UNC-13-4A, ISOFORM B | 27 | 205 | 4.6E-160 |
| 3 | g1828.t1 | PANTHER | PTHR45999:SF2 | PROTEIN UNC-13 HOMOLOG 4B | 234 | 345 | 4.6E-160 |
| 6 | g1828.t1 | PANTHER | PTHR45999 | UNC-13-4A, ISOFORM B | 234 | 345 | 4.6E-160 |
| 4 | g1828.t1 | PANTHER | PTHR45999:SF2 | PROTEIN UNC-13 HOMOLOG 4B | 365 | 1062 | 4.6E-160 |
| 7 | g1828.t1 | PANTHER | PTHR45999 | UNC-13-4A, ISOFORM B | 365 | 1062 | 4.6E-160 |
| 1 | g1828.t1 | Pfam | PF00168 | C2 domain | 910 | 1010 | 1.2E-6 |
| 17 | g1828.t1 | ProSiteProfiles | PS51258 | Munc13-homology domain 1 (MHD1) profile. | 551 | 671 | 16.072 |
| 16 | g1828.t1 | ProSiteProfiles | PS51259 | Munc13-homology domain 2 (MHD2) profile. | 770 | 878 | 11.679 |
| 15 | g1828.t1 | ProSiteProfiles | PS50004 | C2 domain profile. | 907 | 1003 | 11.522 |
| 9 | g1828.t1 | SMART | SM00239 | C2_3c | 910 | 1018 | 1.3E-8 |
| 8 | g1828.t1 | SUPERFAMILY | SSF49562 | C2 domain (Calcium/lipid-binding domain, CaLB) | 902 | 1015 | 8.02E-16 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
