General information for g1841.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phosphorylase b kinase gamma catalytic chain, liver/testis isoform.
Parent gene
- The parent gene g1841 is located on chr_3: 13260701..13261369 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1841 | g1841.t21 | TTS | g1841.t21 | 13254002 | 13254002 |
| chr_3 | g1841 | g1841.t21 | isoform | g1841.t21 | 13254268 | 13260812 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon1 | 13254268 | 13254402 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS1 | 13254268 | 13254402 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon2 | 13254475 | 13254633 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS2 | 13254475 | 13254633 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon3 | 13258760 | 13258857 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS3 | 13258760 | 13258857 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon4 | 13258945 | 13259081 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS4 | 13258945 | 13259081 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon5 | 13259167 | 13259257 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS5 | 13259167 | 13259257 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon6 | 13259319 | 13259406 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS6 | 13259319 | 13259406 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon7 | 13259461 | 13259657 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS7 | 13259461 | 13259657 |
| chr_3 | g1841 | g1841.t21 | exon | g1841.t21.exon8 | 13259736 | 13260812 |
| chr_3 | g1841 | g1841.t21 | cds | g1841.t21.CDS8 | 13259736 | 13259799 |
| chr_3 | g1841 | g1841.t21 | TSS | g1841.t21 | 13261336 | 13261336 |
Sequences
>g1841.t21 Gene=g1841 Length=1982
ATGGCAAAAGATGAGCCAGATGATGATACACTTCCTGACAAAGACGCAGCGAAAGGTTTC
TACGCAAAATATGAACCAAAAGAGGTTCTTGGACGGTGAGTTTATTTATAGAATTTACAC
ATGTACGAGTTCATTTTCCGACAAAAGCAACGAATTGACCTTGAGTCAGATTCGCTTTCG
CACTCTGCTTCTTGTTTTTTTTACTTATCACTTTGCATATTAACACATGACAAGAGTCAC
ACTCAAAAAAATCATGTGCCGCAATGATGACGAAATAAAACGAGCCCCAAAAATTTCAAC
CAATTGCTATGAGAAAGAAAAAAAAATATTCCATTCAACTTCTTCTTCGTGCCGAAAAAC
TTGTTTCAATTTATCGGGTCTATTTATATTTAGCGCTTTATAAGCAAATAAAAAATTTCC
AATTTTCAGCGTTCTTTTTCTTTTCGTAGAATCAACGAAAGTGCAATAAATTCAAATAAC
AATGAATTGTGTCAGTTCTTATACATATTCATATTATTGCTGAGATCATGTGAAAATTGA
TACAATAATAATACTTGTAGTGCATTAGCTTTATTTAAATTTTGCACACCGGTCGAAAAG
ATTTACAAAGCTCATTAGACATTTGATATTTTTATTATGGAGCTTATAAAAGATATTTAT
TTATTCTAGAAACAAACTGTAATAATAGTAAAAAAATTCCTCTAATCATGCTTTCTCTCA
TTCATATTTCAATGTCATGATCACCATGAATAATAAAAAATGAAAAATTCTATATTTGCG
CGAAAAATATTTAAAGCTACCCAGGCTTGTCTAGTTCTCGATAATTCATTCGATTATTGT
TTGAAAATAGCTACAAAACAGGTGTGTGAAAGTAAATTATTTCTTATCAATACTTTTTTA
TTTTTAGTGGCATTTCATCGGTCGTACGTCGATGTATTGATAAGGAAACTGGTAAAGAGT
ATGCGGCAAAGATTATTGATTTGGGAGCATCGGACGTAAATGATTCACATCAGATGCTCG
AAGCGACTCGTCAAGAAATTAATATTTTGAGGCAAGTTATGGGTCATCCTTATATAATTG
AATTGCAAGATGTATTCGAATCTGATGCCTTTATTTTCCTTGTTTTTGAGCTATGTCGTC
ATGGTGAATTGTTTGATTATCTTACATCTGTAGTTACATTATCTGAGAAAAAGACACGTT
ATATTATGAGACAAATTTTTGAAGGTGTTGATTATATTCATTCACGCAATATCGTCCATC
GTGACCTGAAACCCGAAAATATACTCCTCGATGACAATCTTAATGTCAAAATCACAGACT
TTGGGTTTGCTCGTCACTTAACCGATGGCATGAAACTTTATGACTTATGTGGCACACCTG
GGTATCTTGCACCCGAAACTCTTAAATGTAACATGTTCGAAGACGCACCAGGCTATTCAA
AAGAAGTCGATATGTGGGCATGTGGTGTTATTATGTTCACACTTCTTGTCGGTTGCCCTC
CATTCTGGCATCGAAAGCAGATGATAATGCTGCGAAATATCATGGAAGGAAAGTACAGTT
TTATGTCACCCGAGTGGTCAGATATTTCAGAGGATCCAAAAGATCTGATAAGAAAATGTC
TTGTGGTTGATCCCGAGAAGCGTATAACTGTTCGCGAAGCTTTGAAGCATCCATTCTTTA
ACACTGTAATGCGTCGTCAAAGTCAATTTAATGCTAGGAAGAAATTTCAATTTGCTATAC
TATGCGTACGAGCTATGATAAGAATACAAAGATTGCGCTACACACCTGAACCGCTCGACA
TCGAAGAAGCCTTACGCGATCCATATCGTGTCAAAGTATTGCGTAAAGTCATCGATGCAT
GTGCTTTCCGCGTTTACGGCCATTGGGTCAAGAAAGGCGAGGGACAGAATCGTGCTGCTC
TCTTTGAAAACCAACCAAAAGTTGAACTTCATAACCTTTACATTCATCAGTTGCAAAAAT
AA
>g1841.t21 Gene=g1841 Length=322
MLEATRQEINILRQVMGHPYIIELQDVFESDAFIFLVFELCRHGELFDYLTSVVTLSEKK
TRYIMRQIFEGVDYIHSRNIVHRDLKPENILLDDNLNVKITDFGFARHLTDGMKLYDLCG
TPGYLAPETLKCNMFEDAPGYSKEVDMWACGVIMFTLLVGCPPFWHRKQMIMLRNIMEGK
YSFMSPEWSDISEDPKDLIRKCLVVDPEKRITVREALKHPFFNTVMRRQSQFNARKKFQF
AILCVRAMIRIQRLRYTPEPLDIEEALRDPYRVKVLRKVIDACAFRVYGHWVKKGEGQNR
AALFENQPKVELHNLYIHQLQK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g1841.t21 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 1 | 45 | 2.4E-13 |
| 13 | g1841.t21 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 46 | 232 | 1.9E-75 |
| 2 | g1841.t21 | PANTHER | PTHR44167 | OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED | 1 | 319 | 8.8E-189 |
| 3 | g1841.t21 | PANTHER | PTHR44167:SF7 | PHOSPHORYLASE KINASE CATALYTIC SUBUNIT GAMMA 1 | 1 | 319 | 8.8E-189 |
| 10 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 51 | 72 | 1.9E-38 |
| 8 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 214 | 225 | 1.9E-38 |
| 5 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 230 | 245 | 1.9E-38 |
| 7 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 246 | 264 | 1.9E-38 |
| 9 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 265 | 279 | 1.9E-38 |
| 4 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 280 | 295 | 1.9E-38 |
| 6 | g1841.t21 | PRINTS | PR01049 | Phosphorylase kinase family signature | 296 | 309 | 1.9E-38 |
| 1 | g1841.t21 | Pfam | PF00069 | Protein kinase domain | 4 | 222 | 6.9E-70 |
| 15 | g1841.t21 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 144 | - |
| 16 | g1841.t21 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 145 | 165 | - |
| 14 | g1841.t21 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 166 | 322 | - |
| 17 | g1841.t21 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 80 | 92 | - |
| 19 | g1841.t21 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 222 | 48.725 |
| 18 | g1841.t21 | SMART | SM00220 | serkin_6 | 3 | 222 | 6.5E-81 |
| 11 | g1841.t21 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 3 | 241 | 6.39E-80 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005978 | glycogen biosynthetic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0004689 | phosphorylase kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0005516 | calmodulin binding | MF |
| GO:0005964 | phosphorylase kinase complex | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed