General information for g194.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase.
Parent gene
- The parent gene g194 is located on chr_3: 1641991..1642590 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g194 | g194.t1 | TSS | g194.t1 | 1640059 | 1640059 |
| chr_3 | g194 | g194.t1 | isoform | g194.t1 | 1640190 | 1641991 |
| chr_3 | g194 | g194.t1 | exon | g194.t1.exon1 | 1640190 | 1640428 |
| chr_3 | g194 | g194.t1 | cds | g194.t1.CDS1 | 1640190 | 1640428 |
| chr_3 | g194 | g194.t1 | exon | g194.t1.exon2 | 1640497 | 1641630 |
| chr_3 | g194 | g194.t1 | cds | g194.t1.CDS2 | 1640497 | 1641630 |
| chr_3 | g194 | g194.t1 | exon | g194.t1.exon3 | 1641916 | 1641991 |
| chr_3 | g194 | g194.t1 | cds | g194.t1.CDS3 | 1641916 | 1641991 |
| chr_3 | g194 | g194.t1 | TTS | g194.t1 | 1642202 | 1642202 |
Sequences
>g194.t1 Gene=g194 Length=1449
ATGTTGTTTTGTCCGTGTGCTTTGTTAAATCTCGTATATATAGGAATTTTCATCGGAAGT
TTACTGATTTTCGCATTAATTTTCCTCGTCATCATATTAAAACAAATTATCAGTAAAGAG
AAAAATAAAAGACTTGCAACATCATCACAGCAATGTATTGGCATTTTTCATCCATATTGT
AATGCTGGTGGAGGAGGCGAAAGAGTTTTATGGTGTGCTATTCGAGCATTGCAAAACACA
TATGAAGGAACTCGATTCATATTGTACACAGGCGATAAAGAAGCGTCACCCAAACAAATT
TTATCAAAATGCCGTAACAATTTTAATATCGAAATTGATGCTAATCGATTAGAATTTATC
TTTTTAAACCAAAGAAAATGGGTTGAAGCAGAAAAATATCCCTTTTTCACTCTTCTTGGT
CAAAGTTTAGGATCGATGATTTTAGGCTTTGAAGCATTACTTAAGTTTCAACCTGATATT
TATATTGATACAATGGGTTATGCATTTACGTATCCTATTTTTAAATACATTGGTAAAGCT
CGTGTTTCTTCATATACACACTATCCGACAATAAGTACTGACATGTTAAGACGAGTGGCA
AATAGAACGTTAACTTATAATAATCGAAGTCATGTAGCAAGAAATCCATTCCTCACATGG
GCAAAACTTTCATATTATCGTCTCTTTGCATCATTCTATGGTATTGTTGGACGATGCGCA
GAAACAGTCATGGTAAATTCTTCATGGACTGAAAATCACATCAATTCTATATGGAATATA
CCACTTAAAACGCATCGCGTTTATCCACCATGTGAAGTATCTCATTTGAAAGAGATTCAA
CATGTACCAAGTAGTGATGGAAACATTTATATTCTGTCAGTTGGACAATATCGGCCTGAA
AAAGATCATCCTTTGATTCTCCAAAGCCTCTACGAACTGAGAACTTTGCTTGAGCGTGAT
GAAGAGCTATGGGAAAGAATTCGTTTAATTTTTGTCGGCTCATGTCGTAATGATGAGGAT
CTCGAACGTGTTCAGAATTTGCGAGATTTCAGTCGACACTTGGCATTAGATGAACATGTT
GAATTCAAAGTGAATGCCTCTTATCAAGAATTGATTCAATGTTACCAAAAATCGCTAATT
GGTATTCATGCAATGTGGAATGAACATTTCGGCATAAGTGTTGTTGAACTAATGGCTGCT
GGTCTTATCATGATTGCGAATAGATCGGGTGGACCGAGAATGGATATAATTGAAACATCA
GAAGGTAGTCAAACTGGTTACCTTGCCGATAGCGCTGAAGACTATGCTCGCTGTCTAGAA
ACAATTTTATACAACAGCAGAGAGCAAAATGATAAAATTAGAAATGCTGCAAGGGCATCG
GTTGATCGCTTTTCCGAGGTGGAATTTGAAAAGAATTTTCTACGAGTCGTCGGTCCGATG
TTTGACTAA
>g194.t1 Gene=g194 Length=482
MLFCPCALLNLVYIGIFIGSLLIFALIFLVIILKQIISKEKNKRLATSSQQCIGIFHPYC
NAGGGGERVLWCAIRALQNTYEGTRFILYTGDKEASPKQILSKCRNNFNIEIDANRLEFI
FLNQRKWVEAEKYPFFTLLGQSLGSMILGFEALLKFQPDIYIDTMGYAFTYPIFKYIGKA
RVSSYTHYPTISTDMLRRVANRTLTYNNRSHVARNPFLTWAKLSYYRLFASFYGIVGRCA
ETVMVNSSWTENHINSIWNIPLKTHRVYPPCEVSHLKEIQHVPSSDGNIYILSVGQYRPE
KDHPLILQSLYELRTLLERDEELWERIRLIFVGSCRNDEDLERVQNLRDFSRHLALDEHV
EFKVNASYQELIQCYQKSLIGIHAMWNEHFGISVVELMAAGLIMIANRSGGPRMDIIETS
EGSQTGYLADSAEDYARCLETILYNSREQNDKIRNAARASVDRFSEVEFEKNFLRVVGPM
FD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g194.t1 | CDD | cd03806 | GT4_ALG11-like | 53 | 472 | 0.0 |
| 6 | g194.t1 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 271 | 464 | 8.0E-29 |
| 3 | g194.t1 | PANTHER | PTHR45919 | GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE | 14 | 481 | 1.0E-185 |
| 2 | g194.t1 | Pfam | PF15924 | ALG11 mannosyltransferase N-terminus | 51 | 258 | 9.8E-94 |
| 1 | g194.t1 | Pfam | PF00534 | Glycosyl transferases group 1 | 280 | 457 | 5.9E-23 |
| 8 | g194.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 11 | - |
| 9 | g194.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 33 | - |
| 7 | g194.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 34 | 482 | - |
| 5 | g194.t1 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 55 | 475 | 3.81E-41 |
| 4 | g194.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004377 | GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity | MF |
| GO:0016757 | glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03844 (alpha-1,2-mannosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
