Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g194 g194.t2 TSS g194.t2 1640059 1640059
chr_3 g194 g194.t2 isoform g194.t2 1640190 1640804
chr_3 g194 g194.t2 exon g194.t2.exon1 1640190 1640428
chr_3 g194 g194.t2 cds g194.t2.CDS1 1640190 1640428
chr_3 g194 g194.t2 exon g194.t2.exon2 1640497 1640804
chr_3 g194 g194.t2 cds g194.t2.CDS2 1640497 1640803
chr_3 g194 g194.t2 TTS g194.t2 NA NA

Sequences

>g194.t2 Gene=g194 Length=547
ATGTTGTTTTGTCCGTGTGCTTTGTTAAATCTCGTATATATAGGAATTTTCATCGGAAGT
TTACTGATTTTCGCATTAATTTTCCTCGTCATCATATTAAAACAAATTATCAGTAAAGAG
AAAAATAAAAGACTTGCAACATCATCACAGCAATGTATTGGCATTTTTCATCCATATTGT
AATGCTGGTGGAGGAGGCGAAAGAGTTTTATGGTGTGCTATTCGAGCATTGCAAAACACA
TATGAAGGAACTCGATTCATATTGTACACAGGCGATAAAGAAGCGTCACCCAAACAAATT
TTATCAAAATGCCGTAACAATTTTAATATCGAAATTGATGCTAATCGATTAGAATTTATC
TTTTTAAACCAAAGAAAATGGGTTGAAGCAGAAAAATATCCCTTTTTCACTCTTCTTGGT
CAAAGTTTAGGATCGATGATTTTAGGCTTTGAAGCATTACTTAAGTTTCAACCTGATATT
TATATTGATACAATGGGTTATGCATTTACGTATCCTATTTTTAAATACATTGGTAAAGCT
CGTGTTT

>g194.t2 Gene=g194 Length=182
MLFCPCALLNLVYIGIFIGSLLIFALIFLVIILKQIISKEKNKRLATSSQQCIGIFHPYC
NAGGGGERVLWCAIRALQNTYEGTRFILYTGDKEASPKQILSKCRNNFNIEIDANRLEFI
FLNQRKWVEAEKYPFFTLLGQSLGSMILGFEALLKFQPDIYIDTMGYAFTYPIFKYIGKA
RV

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g194.t2 PANTHER PTHR45919 GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE 14 182 2.1E-59
1 g194.t2 Pfam PF15924 ALG11 mannosyltransferase N-terminus 51 182 2.6E-56
7 g194.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 11 -
9 g194.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 12 33 -
4 g194.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 34 132 -
8 g194.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 133 154 -
6 g194.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 155 159 -
10 g194.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 160 178 -
5 g194.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 179 182 -
3 g194.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 11 33 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004377 GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values