General information for g2050.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Hypoxia up-regulated protein 1.
Parent gene
- The parent gene g2050 is located on chr_3: 14832875..14833931 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2050 | g2050.t1 | TSS | g2050.t1 | 14830342 | 14830342 |
| chr_3 | g2050 | g2050.t1 | isoform | g2050.t1 | 14830634 | 14833535 |
| chr_3 | g2050 | g2050.t1 | exon | g2050.t1.exon1 | 14830634 | 14830744 |
| chr_3 | g2050 | g2050.t1 | cds | g2050.t1.CDS1 | 14830634 | 14830744 |
| chr_3 | g2050 | g2050.t1 | exon | g2050.t1.exon2 | 14830818 | 14833535 |
| chr_3 | g2050 | g2050.t1 | cds | g2050.t1.CDS2 | 14830818 | 14833535 |
| chr_3 | g2050 | g2050.t1 | TTS | g2050.t1 | 14833940 | 14833940 |
Sequences
>g2050.t1 Gene=g2050 Length=2829
ATGCGAGTCTCCACTAGTTTAGTCGTAATTCTATCACTAGCAAGCATTCTCATACAAAAT
GTTTCTGGTGCTGCAGTTATGTCCATCGATATTGGTAGTGAATGGATGAAAGTTGGTGTA
GTTTCGCCTGGAATGTCTATGGAAATAGCACTTAATAAGGAATCAAAGAGAAAAACACCT
AGTGTTATTGCATTTAGAGAAAAATCTAGATTTTTCGGTGATGAAGCTATCACACTTGCT
GCTAGATTCCCATCAAGTAGCTATGGCTATCTGCTAGATCTTTTAGGAAAAGATATTTCA
AATCCAATTGTTGAATTATATAAAAAAAGATTTCCTTACTATGAGATTGTGCCTGATTCA
GTTCGCAATACTGTAAGTTTCAAAAACGGTGATGCAGTATATACAATTGAAGAGCTACTT
GCTCAATTACTTGAACGTGCACAATTATTCGCTCAAGATGCAACAGGACAACCGATTCAA
GAATGCGTAATAACAGTACCCGGCTTTTTCGGTCAAGCCGAACGTTTAGCCTTACTTTCC
GCTGCCAAATTAGCAAATCTCAAAGTACTACAATTGATGAATGATTACTCAGCCGTTGCT
CTCAATTACGGCATTTTCCAACGCAAGAATATAAATGAGACAGCACACTATGTTATGTTC
TATGACATGGGCGCAAGTAAAACCACAGTTTCAATTGTTAGTTATCAATTAGTAAAAGAT
AAAGTATTGCGTGAAACACTTCCAGTTGTACAAATTGTTGGTGTGGGATATGATAGAACA
TTAGGCGGTTTAGAAATGCAGTTGAGATTGCGTGATTATTTAGCTGATGCCTTTAATGAT
ATGAAAAAGACAAAACAAAATGTTTATGAAAGTCCACGTGCTATGGCCAAGTTATTAAAA
GAAGCAGGTCGTGTAAAAAATGTATTATCAGCAAATGTAGACCATTATGCACAAATTGAA
GGATTGTTAGATGAGCAAGATTTTAAATTTATGGTTACACGTGAAAAATTTGAAGAGCTC
TGTGCCGATTTATTTGATCGTGTAGCAGCACCTGTTCAAAAAGCACTCGATGCGGCCGGT
CTCACAATTGACGTCATAAATCAATTCATTCTTTTTGGAGGAAATACTCGCACACCAAAA
ATTCAAGAAATTTTGAAAAAGAAAACCAATATGGAGCTTTCAAAGAATTTAAATGCTGAT
GAAGCAGCAACAATGGGTGCTGTTTATCGTGCAGCTGATCTCGCAACAGGATTTAAAGTG
AAAAAGTTTATTGTCAAAGATGCCGTTCTATTTCCAATTAATGTTGTATTTGGTCGAGAG
GGTGACAATAAGAAATTAGTGACAAGAACTCTTTTTGGACCAATGAATTCTTATCCACAG
AAGAAAGTTATTACATTTAATAAGCATAAACAAGATTTTGGTTTTACAGTAAATTATGCT
GATTTAGATTATTTGGGTGCAAAAGAAGTTTTAAATGTGGGCTCATTAAATATCAGCAAA
GTCGAGTTGACAAGTGTTACAAAACTATTTGAGGAAAAAATTAAGGAAGGCATCGATTCG
AAAGGAATTAAAGCTCATTTTGCTCTCGATGATTCTGGTGTATTTTCTCTTGTTAATGTA
GAATTAATTTTAGAAAAGGCTGCAGAGAGTGTTGAAGAAAAGGAAGAAGAAGGTGCATTC
AGTAAATTGGGATCAACAATTAGTAAACTATTTGGTGGTGATAAAGAGGAAGCTCCTGTT
GGAACAAATGAAACTGAAAAGGAGAAAACAACAACTGAAGAAGAAACTACCACAACACCT
ATTCCTCCACCCACTGCTGCAAATGATACTGAAAACGATTCAAAAGAAAAAGAAGATAAA
ACTAAAGAATCAAAGAATGGTAAAAATGATACCACTACAACAGACCAAAAATCAAAAGTT
ATTACTGTTAAAGAACCAGTCGAATCGCAAAGTGAACAATTATTTATAAAAACACTCGAT
GATAATCAATTTTCAGAATCATTAGCAAGAATTTTAGAAATTCAAATGCTTGAACGTGAA
AAATTAAGACGTGAACATGCAGTAAATTCACTTGAATCACATGTAATTGAAGCACAACAG
CGTTTTGAAGAAGAAGAATACATTACTTGTGCAACTGAAAAAGAATTGGAAGAAATAAAG
AAAATGTGCAGTGAAATTAGTGACTGGATTTATGAGGATGGCATTGATGCAGCGGTTGAA
GTATTTGAGGAAAAATTGGCATCATTGACTAAAAAGACGAATGAAGTTTATGCGAGACAT
TGGGAGCATAATGAAAGACCTGAGGCAATTAAAGCTTTAGCTGGAATGATTAATGGAAGC
GAACACTTTTTGGCTACTGCTCGAAATCTTACAAAAGATCCTGAAAAAGGAGATGTTTTC
ACTGAAAAAGAAGTCGAAGACTTAGCAAAGGCTATTCAAGATGTCATTGAATGGCGTGAT
AAAGAGGTTGAAGCACAAGAAAAGTTGCCTAGAAATGCTCCCATCAAACTAACTGTGAAA
GCTTTGACTGATAAAATGGCTTTTCTAGATAGAGAAGTGAAATATCTTGTTAATAAAATA
AAAAGATGGCGACCTAAAGAGAAGACTAAAGAAAAAACTATTCCAGTAAATGAAACTCAA
GATGAAGCGCCAGTAGAAGAAAATAATGGCAAGCAAGAAGAGGGTACAACTACTGATGAA
AAAGAACAAGATGTTGAACAGATTGAAGAGCCTGTTATTGTAGAACAAAATGAGAAAGAA
TCAATTGAACCAACAACATCAACGGAAGCTACAGAAAGTGAAAACAAAGAAGAACATTCA
GAACTTTAA
>g2050.t1 Gene=g2050 Length=942
MRVSTSLVVILSLASILIQNVSGAAVMSIDIGSEWMKVGVVSPGMSMEIALNKESKRKTP
SVIAFREKSRFFGDEAITLAARFPSSSYGYLLDLLGKDISNPIVELYKKRFPYYEIVPDS
VRNTVSFKNGDAVYTIEELLAQLLERAQLFAQDATGQPIQECVITVPGFFGQAERLALLS
AAKLANLKVLQLMNDYSAVALNYGIFQRKNINETAHYVMFYDMGASKTTVSIVSYQLVKD
KVLRETLPVVQIVGVGYDRTLGGLEMQLRLRDYLADAFNDMKKTKQNVYESPRAMAKLLK
EAGRVKNVLSANVDHYAQIEGLLDEQDFKFMVTREKFEELCADLFDRVAAPVQKALDAAG
LTIDVINQFILFGGNTRTPKIQEILKKKTNMELSKNLNADEAATMGAVYRAADLATGFKV
KKFIVKDAVLFPINVVFGREGDNKKLVTRTLFGPMNSYPQKKVITFNKHKQDFGFTVNYA
DLDYLGAKEVLNVGSLNISKVELTSVTKLFEEKIKEGIDSKGIKAHFALDDSGVFSLVNV
ELILEKAAESVEEKEEEGAFSKLGSTISKLFGGDKEEAPVGTNETEKEKTTTEEETTTTP
IPPPTAANDTENDSKEKEDKTKESKNGKNDTTTTDQKSKVITVKEPVESQSEQLFIKTLD
DNQFSESLARILEIQMLEREKLRREHAVNSLESHVIEAQQRFEEEEYITCATEKELEEIK
KMCSEISDWIYEDGIDAAVEVFEEKLASLTKKTNEVYARHWEHNERPEAIKALAGMINGS
EHFLATARNLTKDPEKGDVFTEKEVEDLAKAIQDVIEWRDKEVEAQEKLPRNAPIKLTVK
ALTDKMAFLDREVKYLVNKIKRWRPKEKTKEKTIPVNETQDEAPVEENNGKQEEGTTTDE
KEQDVEQIEEPVIVEQNEKESIEPTTSTEATESENKEEHSEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 26 | g2050.t1 | CDD | cd10230 | HYOU1-like_NBD | 27 | 412 | 2.07276E-175 |
| 20 | g2050.t1 | Coils | Coil | Coil | 537 | 557 | - |
| 18 | g2050.t1 | Gene3D | G3DSA:3.30.420.40 | - | 26 | 412 | 2.8E-119 |
| 17 | g2050.t1 | Gene3D | G3DSA:3.30.420.40 | - | 209 | 394 | 2.8E-119 |
| 16 | g2050.t1 | Gene3D | G3DSA:3.90.640.10 | Actin; Chain A | 262 | 347 | 2.8E-119 |
| 15 | g2050.t1 | Gene3D | G3DSA:2.60.34.10 | Substrate Binding Domain Of DNAk; Chain A | 419 | 565 | 1.9E-31 |
| 19 | g2050.t1 | Gene3D | G3DSA:1.20.1270.10 | - | 659 | 772 | 6.9E-15 |
| 32 | g2050.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 571 | 640 | - |
| 34 | g2050.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 579 | 594 | - |
| 31 | g2050.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 595 | 609 | - |
| 33 | g2050.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 610 | 632 | - |
| 29 | g2050.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 867 | 942 | - |
| 30 | g2050.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 884 | 902 | - |
| 2 | g2050.t1 | PANTHER | PTHR45639:SF3 | HYPOXIA UP-REGULATED PROTEIN 1 | 13 | 907 | 2.1E-264 |
| 3 | g2050.t1 | PANTHER | PTHR45639 | HSC70CB, ISOFORM G-RELATED | 13 | 907 | 2.1E-264 |
| 8 | g2050.t1 | PRINTS | PR00301 | 70kDa heat shock protein signature | 25 | 38 | 3.0E-18 |
| 7 | g2050.t1 | PRINTS | PR00301 | 70kDa heat shock protein signature | 54 | 66 | 3.0E-18 |
| 5 | g2050.t1 | PRINTS | PR00301 | 70kDa heat shock protein signature | 162 | 182 | 3.0E-18 |
| 9 | g2050.t1 | PRINTS | PR00301 | 70kDa heat shock protein signature | 366 | 382 | 3.0E-18 |
| 6 | g2050.t1 | PRINTS | PR00301 | 70kDa heat shock protein signature | 397 | 417 | 3.0E-18 |
| 4 | g2050.t1 | PRINTS | PR00301 | 70kDa heat shock protein signature | 520 | 536 | 3.0E-18 |
| 1 | g2050.t1 | Pfam | PF00012 | Hsp70 protein | 27 | 653 | 1.6E-81 |
| 22 | g2050.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 23 | g2050.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 24 | g2050.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 18 | - |
| 25 | g2050.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 23 | - |
| 21 | g2050.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 942 | - |
| 11 | g2050.t1 | SUPERFAMILY | SSF53067 | Actin-like ATPase domain | 26 | 206 | 9.71E-40 |
| 12 | g2050.t1 | SUPERFAMILY | SSF53067 | Actin-like ATPase domain | 216 | 412 | 1.44E-39 |
| 10 | g2050.t1 | SUPERFAMILY | SSF100934 | Heat shock protein 70kD (HSP70), C-terminal subdomain | 687 | 768 | 5.3E-13 |
| 14 | g2050.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 27 | g2050.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 24 | - |
| 13 | g2050.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 24 | - |
| 28 | g2050.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K09486 (hypoxia up-regulated 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
