General information for g2050.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Hypoxia up-regulated protein 1 .
Parent gene
- The parent gene g2050 is located on chr_3: 14832875..14833931 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2050 | g2050.t3 | isoform | g2050.t3 | 14830328 | 14832550 |
| chr_3 | g2050 | g2050.t3 | exon | g2050.t3.exon1 | 14830328 | 14830561 |
| chr_3 | g2050 | g2050.t3 | TSS | g2050.t3 | 14830342 | 14830342 |
| chr_3 | g2050 | g2050.t3 | exon | g2050.t3.exon2 | 14830623 | 14830744 |
| chr_3 | g2050 | g2050.t3 | cds | g2050.t3.CDS1 | 14830634 | 14830744 |
| chr_3 | g2050 | g2050.t3 | exon | g2050.t3.exon3 | 14830818 | 14832550 |
| chr_3 | g2050 | g2050.t3 | cds | g2050.t3.CDS2 | 14830818 | 14832548 |
| chr_3 | g2050 | g2050.t3 | TTS | g2050.t3 | 14832546 | 14832546 |
Sequences
>g2050.t3 Gene=g2050 Length=2089
AAAGAGAGATTTCACTGTATTACTAGTAGAAAGTTGAAGTTAAAAAAGTTCTCAAATAAA
TACAAGATTTTATTAATTTACGTGTCTCATTAGCGTGTTTTTGGCTCAATACTCGTTTAG
CCATCACACAATTAAGTTGAATCTACTTGAATTTATATAATTTCAATTGATTTCTTTAAA
AAATCTTTGATACAAAAAAGAACACGTCATATTAATTCTAATCCTCAAAGGTTTGAGCTG
CCAAAATGCGAGTCTCCACTAGTTTAGTCGTAATTCTATCACTAGCAAGCATTCTCATAC
AAAATGTTTCTGGTGCTGCAGTTATGTCCATCGATATTGGTAGTGAATGGATGAAAGTTG
GTGTAGTTTCGCCTGGAATGTCTATGGAAATAGCACTTAATAAGGAATCAAAGAGAAAAA
CACCTAGTGTTATTGCATTTAGAGAAAAATCTAGATTTTTCGGTGATGAAGCTATCACAC
TTGCTGCTAGATTCCCATCAAGTAGCTATGGCTATCTGCTAGATCTTTTAGGAAAAGATA
TTTCAAATCCAATTGTTGAATTATATAAAAAAAGATTTCCTTACTATGAGATTGTGCCTG
ATTCAGTTCGCAATACTGTAAGTTTCAAAAACGGTGATGCAGTATATACAATTGAAGAGC
TACTTGCTCAATTACTTGAACGTGCACAATTATTCGCTCAAGATGCAACAGGACAACCGA
TTCAAGAATGCGTAATAACAGTACCCGGCTTTTTCGGTCAAGCCGAACGTTTAGCCTTAC
TTTCCGCTGCCAAATTAGCAAATCTCAAAGTACTACAATTGATGAATGATTACTCAGCCG
TTGCTCTCAATTACGGCATTTTCCAACGCAAGAATATAAATGAGACAGCACACTATGTTA
TGTTCTATGACATGGGCGCAAGTAAAACCACAGTTTCAATTGTTAGTTATCAATTAGTAA
AAGATAAAGTATTGCGTGAAACACTTCCAGTTGTACAAATTGTTGGTGTGGGATATGATA
GAACATTAGGCGGTTTAGAAATGCAGTTGAGATTGCGTGATTATTTAGCTGATGCCTTTA
ATGATATGAAAAAGACAAAACAAAATGTTTATGAAAGTCCACGTGCTATGGCCAAGTTAT
TAAAAGAAGCAGGTCGTGTAAAAAATGTATTATCAGCAAATGTAGACCATTATGCACAAA
TTGAAGGATTGTTAGATGAGCAAGATTTTAAATTTATGGTTACACGTGAAAAATTTGAAG
AGCTCTGTGCCGATTTATTTGATCGTGTAGCAGCACCTGTTCAAAAAGCACTCGATGCGG
CCGGTCTCACAATTGACGTCATAAATCAATTCATTCTTTTTGGAGGAAATACTCGCACAC
CAAAAATTCAAGAAATTTTGAAAAAGAAAACCAATATGGAGCTTTCAAAGAATTTAAATG
CTGATGAAGCAGCAACAATGGGTGCTGTTTATCGTGCAGCTGATCTCGCAACAGGATTTA
AAGTGAAAAAGTTTATTGTCAAAGATGCCGTTCTATTTCCAATTAATGTTGTATTTGGTC
GAGAGGGTGACAATAAGAAATTAGTGACAAGAACTCTTTTTGGACCAATGAATTCTTATC
CACAGAAGAAAGTTATTACATTTAATAAGCATAAACAAGATTTTGGTTTTACAGTAAATT
ATGCTGATTTAGATTATTTGGGTGCAAAAGAAGTTTTAAATGTGGGCTCATTAAATATCA
GCAAAGTCGAGTTGACAAGTGTTACAAAACTATTTGAGGAAAAAATTAAGGAAGGCATCG
ATTCGAAAGGAATTAAAGCTCATTTTGCTCTCGATGATTCTGGTGTATTTTCTCTTGTTA
ATGTAGAATTAATTTTAGAAAAGGCTGCAGAGAGTGTTGAAGAAAAGGAAGAAGAAGGTG
CATTCAGTAAATTGGGATCAACAATTAGTAAACTATTTGGTGGTGATAAAGAGGAAGCTC
CTGTTGGAACAAATGAAACTGAAAAGGAGAAAACAACAACTGAAGAAGAAACTACCACAA
CACCTATTCCTCCACCCACTGCTGCAAATGATACTGAAAACGATTCAAA
>g2050.t3 Gene=g2050 Length=614
MRVSTSLVVILSLASILIQNVSGAAVMSIDIGSEWMKVGVVSPGMSMEIALNKESKRKTP
SVIAFREKSRFFGDEAITLAARFPSSSYGYLLDLLGKDISNPIVELYKKRFPYYEIVPDS
VRNTVSFKNGDAVYTIEELLAQLLERAQLFAQDATGQPIQECVITVPGFFGQAERLALLS
AAKLANLKVLQLMNDYSAVALNYGIFQRKNINETAHYVMFYDMGASKTTVSIVSYQLVKD
KVLRETLPVVQIVGVGYDRTLGGLEMQLRLRDYLADAFNDMKKTKQNVYESPRAMAKLLK
EAGRVKNVLSANVDHYAQIEGLLDEQDFKFMVTREKFEELCADLFDRVAAPVQKALDAAG
LTIDVINQFILFGGNTRTPKIQEILKKKTNMELSKNLNADEAATMGAVYRAADLATGFKV
KKFIVKDAVLFPINVVFGREGDNKKLVTRTLFGPMNSYPQKKVITFNKHKQDFGFTVNYA
DLDYLGAKEVLNVGSLNISKVELTSVTKLFEEKIKEGIDSKGIKAHFALDDSGVFSLVNV
ELILEKAAESVEEKEEEGAFSKLGSTISKLFGGDKEEAPVGTNETEKEKTTTEEETTTTP
IPPPTAANDTENDS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 24 | g2050.t3 | CDD | cd10230 | HYOU1-like_NBD | 27 | 412 | 3.1489E-180 |
| 18 | g2050.t3 | Coils | Coil | Coil | 537 | 557 | - |
| 17 | g2050.t3 | Gene3D | G3DSA:3.30.420.40 | - | 26 | 412 | 9.3E-120 |
| 16 | g2050.t3 | Gene3D | G3DSA:3.30.420.40 | - | 209 | 394 | 9.3E-120 |
| 15 | g2050.t3 | Gene3D | G3DSA:3.90.640.10 | Actin; Chain A | 262 | 347 | 9.3E-120 |
| 14 | g2050.t3 | Gene3D | G3DSA:2.60.34.10 | Substrate Binding Domain Of DNAk; Chain A | 419 | 565 | 9.8E-32 |
| 28 | g2050.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 571 | 614 | - |
| 29 | g2050.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 579 | 594 | - |
| 27 | g2050.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 595 | 614 | - |
| 2 | g2050.t3 | PANTHER | PTHR45639:SF3 | HYPOXIA UP-REGULATED PROTEIN 1 | 13 | 597 | 3.2E-211 |
| 3 | g2050.t3 | PANTHER | PTHR45639 | HSC70CB, ISOFORM G-RELATED | 13 | 597 | 3.2E-211 |
| 8 | g2050.t3 | PRINTS | PR00301 | 70kDa heat shock protein signature | 25 | 38 | 3.0E-19 |
| 7 | g2050.t3 | PRINTS | PR00301 | 70kDa heat shock protein signature | 54 | 66 | 3.0E-19 |
| 5 | g2050.t3 | PRINTS | PR00301 | 70kDa heat shock protein signature | 162 | 182 | 3.0E-19 |
| 9 | g2050.t3 | PRINTS | PR00301 | 70kDa heat shock protein signature | 366 | 382 | 3.0E-19 |
| 6 | g2050.t3 | PRINTS | PR00301 | 70kDa heat shock protein signature | 397 | 417 | 3.0E-19 |
| 4 | g2050.t3 | PRINTS | PR00301 | 70kDa heat shock protein signature | 520 | 536 | 3.0E-19 |
| 1 | g2050.t3 | Pfam | PF00012 | Hsp70 protein | 27 | 571 | 2.4E-81 |
| 20 | g2050.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 21 | g2050.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 22 | g2050.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 18 | - |
| 23 | g2050.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 23 | - |
| 19 | g2050.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 614 | - |
| 10 | g2050.t3 | SUPERFAMILY | SSF53067 | Actin-like ATPase domain | 26 | 206 | 4.53E-40 |
| 11 | g2050.t3 | SUPERFAMILY | SSF53067 | Actin-like ATPase domain | 216 | 412 | 6.7E-40 |
| 13 | g2050.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 25 | g2050.t3 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 24 | - |
| 12 | g2050.t3 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 24 | - |
| 26 | g2050.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K09486 (hypoxia up-regulated 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed