General information for g2094.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g2094 is located on chr_3: 15153714..15155659 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2094 | g2094.t1 | isoform | g2094.t1 | 15153714 | 15155659 |
| chr_3 | g2094 | g2094.t1 | exon | g2094.t1.exon1 | 15153714 | 15154993 |
| chr_3 | g2094 | g2094.t1 | cds | g2094.t1.CDS1 | 15153714 | 15154993 |
| chr_3 | g2094 | g2094.t1 | exon | g2094.t1.exon2 | 15155054 | 15155139 |
| chr_3 | g2094 | g2094.t1 | cds | g2094.t1.CDS2 | 15155054 | 15155139 |
| chr_3 | g2094 | g2094.t1 | exon | g2094.t1.exon3 | 15155269 | 15155295 |
| chr_3 | g2094 | g2094.t1 | cds | g2094.t1.CDS3 | 15155269 | 15155295 |
| chr_3 | g2094 | g2094.t1 | exon | g2094.t1.exon4 | 15155349 | 15155659 |
| chr_3 | g2094 | g2094.t1 | cds | g2094.t1.CDS4 | 15155349 | 15155659 |
| chr_3 | g2094 | g2094.t1 | TSS | g2094.t1 | NA | NA |
| chr_3 | g2094 | g2094.t1 | TTS | g2094.t1 | NA | NA |
Sequences
>g2094.t1 Gene=g2094 Length=1704
ATGCTACAATCAACAGAAATGGGATTCAAATCTATAATAGCAAAAAACAATCATTTGTTT
TCAAGAATTTTCTGGTTGATTGCTTTTATCACATCAATTATTCTTTTGATTTATTCTTCA
TTTTGGTTGATTTGGAAATATAATTATGAGCCAGATTTTGGTGTAAAAATTATTCAAAGA
CCAATGCGGGAAATTCCATTTCCTGCTTTTACGATTTGCTCGCCAGTCTTTGCTAAAAAC
AATCTTGCAAACTACTTGAAACTTGGAAATATTTTCAACTCAAAAGCAAACTTTACAATC
AAAGAACAAAATTATTTTGCTGCAAATTACCAAGTGTGCAATCCATTTGATGATATAGGT
GACTTTCAGACAGTTAAGGATCATGTGATGAATAGAACTGACTTTGACATTGTCAAGCTT
CTAAATGAAAGTTCAATGAACGTCACTGAATTAATACATTTTTGCAGCATTTCAGATGAG
CTTGATTGTGTAAAATCATTCAATCAAATTCTCACTGAGCGTGGTTTCTGTTATAATTTC
AACATGCAAAGCAATGATTTAATTTATAATGATGTGATAAACAAAAAATGGAAAAATTTA
AATTTTCTTAAAAAAATTTTTAATGAAAATTCTGAAAATGATGAAAAAATGACGTGGACA
CTTGATAAAGGCTATTCATCAAAGCATAGAGACAATCAAAATCCATATCGTGCAACAAAA
CACAATCGAATTGGAGTTTACATCAGCCTCAGTCATGAAAATGCTAACAATCCATGTCCT
ATTGTTGGTAAAGTATTTCGCGTTATTTTTCATTTGCCAAATGAAATTCCAACAATTTTT
CATAACGAGCACTTTGTTAGTTATCACTATCAAAAGACTTTTTTCCTAACTGCAACTTCA
TATAGATCAGACAAAGAATTACGCAGATATTCACCAAACATTCGTGGTTGTTATTTTCAA
GATGAAAGAAAATTGAAATTTTTCAAAAGTTATACAAAAACACAATGTGACTATGAATGT
CTCACTAATTATACACTTGAAATTTGCGGTTGTGTACGGTTTTCAATGCCTCACGAATTC
AACACTCCAATTTGCGATCTTGATCAAATGAAATGCTACTCTTATGTTATGTTACGATGG
CCTCAAAATAATGAAGAGAATCACATCGCTTGCAATTGTTTGCCAACTTGTAATTATATC
CAATACTCTTTGAGATATGAGAAGGAAGCTTTTATTGATTATGTCAAAGATTTACTCGGA
CCTGAAGAAAAAACTAAAAATACATCTGATGTTGTCATTTCTGTGAGCTTTGTTGAACAT
TTAGTTATGGAACATCAACGATTTTTATCTTATTCATTTGGAAATTTCATTGCAGATATT
GGTGGATTATTAGGTCTTTTCTTAGGAGCATCTTTAGTGACTTTTATTGAAATCATTTAC
ATGGCAACTCAATTCATCATTAGAAAAATCAAAAAGCAACAAAAAGTTGAAGTTTTTGAT
ATAACAAATGATGCAGAAATGCAAGCTGTGAAAATTCAAAAACTTAGTCAAGTTGTTCAA
ATTTTAGATTTTAAACAAAAAGAATCAAACGTTAAAATTACAAATTTAGAAGCAAGAATG
GAAAAACTGAAAGGAAGAAATGAACAAAATGATGAAATTATAAAGTCTTTGGAGGAAATA
ATTTTTAAAAATCGTAATGATTAA
>g2094.t1 Gene=g2094 Length=567
MLQSTEMGFKSIIAKNNHLFSRIFWLIAFITSIILLIYSSFWLIWKYNYEPDFGVKIIQR
PMREIPFPAFTICSPVFAKNNLANYLKLGNIFNSKANFTIKEQNYFAANYQVCNPFDDIG
DFQTVKDHVMNRTDFDIVKLLNESSMNVTELIHFCSISDELDCVKSFNQILTERGFCYNF
NMQSNDLIYNDVINKKWKNLNFLKKIFNENSENDEKMTWTLDKGYSSKHRDNQNPYRATK
HNRIGVYISLSHENANNPCPIVGKVFRVIFHLPNEIPTIFHNEHFVSYHYQKTFFLTATS
YRSDKELRRYSPNIRGCYFQDERKLKFFKSYTKTQCDYECLTNYTLEICGCVRFSMPHEF
NTPICDLDQMKCYSYVMLRWPQNNEENHIACNCLPTCNYIQYSLRYEKEAFIDYVKDLLG
PEEKTKNTSDVVISVSFVEHLVMEHQRFLSYSFGNFIADIGGLLGLFLGASLVTFIEIIY
MATQFIIRKIKKQQKVEVFDITNDAEMQAVKIQKLSQVVQILDFKQKESNVKITNLEARM
EKLKGRNEQNDEIIKSLEEIIFKNRND
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g2094.t1 | Coils | Coil | Coil | 526 | 560 | - |
| 2 | g2094.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 3 | 510 | 1.5E-48 |
| 3 | g2094.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 3 | 510 | 1.5E-48 |
| 6 | g2094.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 22 | 39 | 2.8E-9 |
| 7 | g2094.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 63 | 79 | 2.8E-9 |
| 5 | g2094.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 331 | 342 | 2.8E-9 |
| 4 | g2094.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 342 | 359 | 2.8E-9 |
| 9 | g2094.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 447 | 461 | 2.8E-9 |
| 8 | g2094.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 461 | 477 | 2.8E-9 |
| 1 | g2094.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 4 | 480 | 1.7E-69 |
| 15 | g2094.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 22 | - |
| 17 | g2094.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 23 | 45 | - |
| 16 | g2094.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 46 | 459 | - |
| 18 | g2094.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 460 | 482 | - |
| 14 | g2094.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 483 | 567 | - |
| 12 | g2094.t1 | ProSitePatterns | PS01206 | Amiloride-sensitive sodium channels signature. | 331 | 351 | - |
| 10 | g2094.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 23 | 45 | - |
| 11 | g2094.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 460 | 482 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed