General information for g2095.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g2095 is located on chr_3: 15157910..15159697 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2095 | g2095.t1 | isoform | g2095.t1 | 15157910 | 15159697 |
| chr_3 | g2095 | g2095.t1 | exon | g2095.t1.exon1 | 15157910 | 15158217 |
| chr_3 | g2095 | g2095.t1 | cds | g2095.t1.CDS1 | 15157910 | 15158217 |
| chr_3 | g2095 | g2095.t1 | exon | g2095.t1.exon2 | 15158275 | 15158360 |
| chr_3 | g2095 | g2095.t1 | cds | g2095.t1.CDS2 | 15158275 | 15158360 |
| chr_3 | g2095 | g2095.t1 | exon | g2095.t1.exon3 | 15158415 | 15159697 |
| chr_3 | g2095 | g2095.t1 | cds | g2095.t1.CDS3 | 15158415 | 15159697 |
| chr_3 | g2095 | g2095.t1 | TSS | g2095.t1 | NA | NA |
| chr_3 | g2095 | g2095.t1 | TTS | g2095.t1 | NA | NA |
Sequences
>g2095.t1 Gene=g2095 Length=1677
ATGGAAGAGAAAAATAATGAAACTGGTTTTCAAGCGATAATTAATAAAAGAAATAGTTGC
TTAACAAGGTTTTTCTGGTTAATTGCTTTAAGTGCATCAATCGGAGGACTTTTATTTCAT
TTAGTAGTTCTTTACAGAAAGTATGGATATGAGCCTGATTTAGGAGTCAAAATTTCACAA
AAACCAATGCGCGATATTCCATTTCCTGCTATCACAATTTGTTCACCAGTTTTTGCAAAA
AACAATATTGTTAATTATAGAAAATTCGGAATGTTTTTTGAAATGACAGGAAAAAATTTA
ACAACTGAACAAAAAAATTATTTTGCTGCAAATTATCAAGTTTGTAATCCATCTGATGAA
ATTGGTGACTATGAAATTGTTTCACATTTTCTAAAGAAAAGAACTGATTTTGATTTGATT
AAATTGGTAGAAGAAACGTCTTTGAATGTCACTGATTTGATGTTAGTTTGTGCTGTTCAT
GGAGTTCCTATAGATTGCACAAAAATTTTTACTCGCGTTTTAACTGACTTTGGTTTATGC
TTTACTTTTAACATGCAAGCTAATGAAATTATTTATAATAGCATTGCTAGTGATTCATTT
AAAGCACGCACACCTGAAACTTGGAATAAAAGTAATAAAACCAATAAAAGGGCAGTTTGG
ACACTCGATAAAAGTTTTCCAAAAGATTTCAAAGATGATCAAATTCCATATCGCGCTGTC
AAGAAAAATCGTCTTTCAATATTATTGAACCTTAAGCAAGAAAACGCTGAAAACTTTTGT
CCAACTATGGGAAAAGTTTTTACAACAATTTTGCATTTGCCTAATGAAGTACCAACAATT
TTTCATGATCAATATTTTACTCAATATAATTATGGAAGAAGGTTTCTTTTAAGTGCAATT
TCATATAAAGCTAACAATGATATTCGAAAATATTCTCCTAAAGCACGCGGATGTTACTTT
GATGATGAAAGAAAATTGAAGTTTTTCAAAACCTACACAAGAACTCATTGCAATTTTGAA
TGTTTAAGTAATTATTCACTAAAAATTTGTGGTTGCGTAAAGTTTTCAATGCCTCATGAT
AAAGATACTCCTATATGTGATCTTGCTGTAAAACAATGCTATGTAGATGCTTTAAACCAA
TGGCCAGAGAAAATTGATGGAAACAAAGTTGTATGCAATTGTTTGCCTACTTGCAATCAT
GTTAGCTATAAACTGAAAAATAGTAAAGAAATTTTAATTGATTATGTCGATGATTTTCTT
GGTTCTGCACTTCGACCTGTAAATTCAACAGATGCAATGCTATCATTTAGGTTTATCGAG
CCTATAGCTACAGAGCATCAAAAATTTATTGCTTATAGATTTGAAAATTTTATGGCAGAC
ATTGGTGGATTATTAGGACTTTTTATGGGATGCTCATTGATAACTTTTGTTGAAATATTT
TACACCATTGCAAGATTATTTTTCGATAAACAAAAGAAGAAAAATGCTGTGGAGAAAATT
GAAATAAACGCAGATGAAAATGAAATGTACAAAAGCAAACAAGTTCATCAAATTTTAAAA
GATTTTCAACTGAATCAAAGAGAAGTGATAAAAAGAATTTTAAAGATTGAGGAAAATGTT
GAACAAACCAAAAGAGAAATTAAATTTTTAAAATCAATGCACACATTTGAACGATAA
>g2095.t1 Gene=g2095 Length=558
MEEKNNETGFQAIINKRNSCLTRFFWLIALSASIGGLLFHLVVLYRKYGYEPDLGVKISQ
KPMRDIPFPAITICSPVFAKNNIVNYRKFGMFFEMTGKNLTTEQKNYFAANYQVCNPSDE
IGDYEIVSHFLKKRTDFDLIKLVEETSLNVTDLMLVCAVHGVPIDCTKIFTRVLTDFGLC
FTFNMQANEIIYNSIASDSFKARTPETWNKSNKTNKRAVWTLDKSFPKDFKDDQIPYRAV
KKNRLSILLNLKQENAENFCPTMGKVFTTILHLPNEVPTIFHDQYFTQYNYGRRFLLSAI
SYKANNDIRKYSPKARGCYFDDERKLKFFKTYTRTHCNFECLSNYSLKICGCVKFSMPHD
KDTPICDLAVKQCYVDALNQWPEKIDGNKVVCNCLPTCNHVSYKLKNSKEILIDYVDDFL
GSALRPVNSTDAMLSFRFIEPIATEHQKFIAYRFENFMADIGGLLGLFMGCSLITFVEIF
YTIARLFFDKQKKKNAVEKIEINADENEMYKSKQVHQILKDFQLNQREVIKRILKIEENV
EQTKREIKFLKSMHTFER
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g2095.t1 | Coils | Coil | Coil | 526 | 553 | - |
| 15 | g2095.t1 | Gene3D | G3DSA:1.10.287.770 | - | 23 | 53 | 3.6E-17 |
| 10 | g2095.t1 | Gene3D | G3DSA:2.60.470.10 | - | 54 | 188 | 3.6E-17 |
| 12 | g2095.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 77 | 148 | 3.6E-17 |
| 11 | g2095.t1 | Gene3D | G3DSA:2.60.470.10 | - | 235 | 450 | 2.1E-33 |
| 13 | g2095.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 252 | 266 | 2.1E-33 |
| 14 | g2095.t1 | Gene3D | G3DSA:1.10.287.820 | - | 310 | 398 | 2.1E-33 |
| 16 | g2095.t1 | Gene3D | G3DSA:1.10.287.770 | - | 451 | 479 | 2.1E-33 |
| 2 | g2095.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 13 | 532 | 2.5E-51 |
| 3 | g2095.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 13 | 532 | 2.5E-51 |
| 4 | g2095.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 64 | 80 | 5.5E-7 |
| 7 | g2095.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 168 | 184 | 5.5E-7 |
| 5 | g2095.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 448 | 462 | 5.5E-7 |
| 6 | g2095.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 462 | 478 | 5.5E-7 |
| 1 | g2095.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 9 | 481 | 4.0E-67 |
| 19 | g2095.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 23 | - |
| 22 | g2095.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 24 | 45 | - |
| 20 | g2095.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 46 | 460 | - |
| 21 | g2095.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 461 | 484 | - |
| 18 | g2095.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 485 | 558 | - |
| 9 | g2095.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 21 | 43 | - |
| 8 | g2095.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 462 | 484 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed