General information for g2118.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mitotic checkpoint protein BUB3.
Parent gene
- The parent gene g2118 is located on chr_3: 15329379..15329895 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2118 | g2118.t1 | TSS | g2118.t1 | 15328729 | 15328729 |
| chr_3 | g2118 | g2118.t1 | isoform | g2118.t1 | 15328810 | 15329855 |
| chr_3 | g2118 | g2118.t1 | exon | g2118.t1.exon1 | 15328810 | 15329007 |
| chr_3 | g2118 | g2118.t1 | cds | g2118.t1.CDS1 | 15328810 | 15329007 |
| chr_3 | g2118 | g2118.t1 | exon | g2118.t1.exon2 | 15329067 | 15329855 |
| chr_3 | g2118 | g2118.t1 | cds | g2118.t1.CDS2 | 15329067 | 15329855 |
| chr_3 | g2118 | g2118.t1 | TTS | g2118.t1 | 15329918 | 15329918 |
Sequences
>g2118.t1 Gene=g2118 Length=987
ATGTCAACTAAAAAAGCAGAAATACAAATAAATTATGCACCGCAAGATACAATTTCATCA
CTGGGTTTTGCTCCAGATAGCTCACAATATTTAATTGCAAGCTCATGGGATGGAACAGTT
CGTTTGTACGACACAACAAATAATGTTATGAGACAAAAATATCTTCACAACTGTCCTGTT
CTTGATGTAGCATTTCAAGACAATGTACATACAATTTCTGGAGGACTTGATGGTGGTTTA
AAACTTTTTGATTTTAATAATAGCAGTGAAACAAATCTCGGTTCACATGATAATGCAATC
AAATGTGTAGAATTCTCAACAAAACTAAATGCTGTTGTCACTGGCAGTTGGGATACAACT
GTAAGAATATGGGATACAAGAGAGAAAAGTTGTGTTGGAACTTATGAACAAAATAATAGT
AAAGTCTATTCACTGAGTGTAGTTGATGAGAAACTGGTTGTAGCTACAAGTGATCGAAAA
GTGCTTATATGGGATTTAAGAAATATGGCAAATTATGTTGCTCGTCGAGAATCATCACTT
AAATATCAAACGCGCTGCATTCGAGTATTTCCCAATAAAGAAGGCTATGTTATGTCTTCA
ATTGAAGGTCGTGTGGCAGTTGAATACTTTGATAATGATCCTGAGATACAAAAGAAAAAG
TTTGCATTTAAATGTCATCGTGCAAAAGAGAATAATATTGAAATGATTTATCCAGTAAAT
GCCATTAGTTTTCATAATGGTTTCTCAACTTTTGCAACTGCAGGATCTGATGGCTATGTC
AGTATTTGGGATGGTTTCAACAAAAAACGATTGTGTCAATTTCATCATTACGAAACTTCA
ATCTCTGCAATTTCATTCAGTAATGATGGTAGTACGCTTGCTATTGGTTGCTCATATCTT
GACGAATTGGAAAAACCTCCGGAACCACTTCCCGAACCATCAATTTATGTACGTTATGTA
AATGAACAAGAAGTCAAACCCAAGTAA
>g2118.t1 Gene=g2118 Length=328
MSTKKAEIQINYAPQDTISSLGFAPDSSQYLIASSWDGTVRLYDTTNNVMRQKYLHNCPV
LDVAFQDNVHTISGGLDGGLKLFDFNNSSETNLGSHDNAIKCVEFSTKLNAVVTGSWDTT
VRIWDTREKSCVGTYEQNNSKVYSLSVVDEKLVVATSDRKVLIWDLRNMANYVARRESSL
KYQTRCIRVFPNKEGYVMSSIEGRVAVEYFDNDPEIQKKKFAFKCHRAKENNIEMIYPVN
AISFHNGFSTFATAGSDGYVSIWDGFNKKRLCQFHHYETSISAISFSNDGSTLAIGCSYL
DELEKPPEPLPEPSIYVRYVNEQEVKPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g2118.t1 | Gene3D | G3DSA:2.130.10.10 | - | 1 | 328 | 5.3E-95 |
| 5 | g2118.t1 | PANTHER | PTHR10971 | MRNA EXPORT FACTOR AND BUB3 | 3 | 328 | 3.5E-132 |
| 6 | g2118.t1 | PANTHER | PTHR10971:SF31 | MITOTIC CHECKPOINT PROTEIN BUB3 | 3 | 328 | 3.5E-132 |
| 7 | g2118.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 31 | 45 | 3.3E-6 |
| 9 | g2118.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 112 | 126 | 3.3E-6 |
| 8 | g2118.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 251 | 265 | 3.3E-6 |
| 4 | g2118.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 18 | 44 | 0.025 |
| 1 | g2118.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 93 | 125 | 2.0E-5 |
| 3 | g2118.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 130 | 165 | 0.12 |
| 2 | g2118.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 224 | 264 | 0.049 |
| 12 | g2118.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 112 | 126 | - |
| 19 | g2118.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 11 | 53 | 8.967 |
| 22 | g2118.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 11 | 53 | 9.005 |
| 18 | g2118.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 93 | 296 | 19.538 |
| 20 | g2118.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 93 | 134 | 14.686 |
| 21 | g2118.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 239 | 264 | 9.172 |
| 16 | g2118.t1 | SMART | SM00320 | WD40_4 | 4 | 44 | 6.9E-5 |
| 15 | g2118.t1 | SMART | SM00320 | WD40_4 | 46 | 84 | 1.4 |
| 14 | g2118.t1 | SMART | SM00320 | WD40_4 | 86 | 125 | 1.2E-5 |
| 17 | g2118.t1 | SMART | SM00320 | WD40_4 | 128 | 165 | 0.071 |
| 13 | g2118.t1 | SMART | SM00320 | WD40_4 | 216 | 264 | 0.0023 |
| 10 | g2118.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 11 | 298 | 3.97E-50 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K02180 (cell cycle arrest protein BUB3)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
