General information for g2118.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mitotic checkpoint protein BUB3.
Parent gene
- The parent gene g2118 is located on chr_3: 15329379..15329895 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2118 | g2118.t2 | TSS | g2118.t2 | 15328729 | 15328729 |
| chr_3 | g2118 | g2118.t2 | isoform | g2118.t2 | 15328810 | 15329855 |
| chr_3 | g2118 | g2118.t2 | exon | g2118.t2.exon1 | 15328810 | 15329007 |
| chr_3 | g2118 | g2118.t2 | exon | g2118.t2.exon2 | 15329135 | 15329855 |
| chr_3 | g2118 | g2118.t2 | cds | g2118.t2.CDS1 | 15329373 | 15329855 |
| chr_3 | g2118 | g2118.t2 | TTS | g2118.t2 | 15329918 | 15329918 |
Sequences
>g2118.t2 Gene=g2118 Length=919
ATGTCAACTAAAAAAGCAGAAATACAAATAAATTATGCACCGCAAGATACAATTTCATCA
CTGGGTTTTGCTCCAGATAGCTCACAATATTTAATTGCAAGCTCATGGGATGGAACAGTT
CGTTTGTACGACACAACAAATAATGTTATGAGACAAAAATATCTTCACAACTGTCCTGTT
CTTGATGTAGCATTTCAATGAAACAAATCTCGGTTCACATGATAATGCAATCAAATGTGT
AGAATTCTCAACAAAACTAAATGCTGTTGTCACTGGCAGTTGGGATACAACTGTAAGAAT
ATGGGATACAAGAGAGAAAAGTTGTGTTGGAACTTATGAACAAAATAATAGTAAAGTCTA
TTCACTGAGTGTAGTTGATGAGAAACTGGTTGTAGCTACAAGTGATCGAAAAGTGCTTAT
ATGGGATTTAAGAAATATGGCAAATTATGTTGCTCGTCGAGAATCATCACTTAAATATCA
AACGCGCTGCATTCGAGTATTTCCCAATAAAGAAGGCTATGTTATGTCTTCAATTGAAGG
TCGTGTGGCAGTTGAATACTTTGATAATGATCCTGAGATACAAAAGAAAAAGTTTGCATT
TAAATGTCATCGTGCAAAAGAGAATAATATTGAAATGATTTATCCAGTAAATGCCATTAG
TTTTCATAATGGTTTCTCAACTTTTGCAACTGCAGGATCTGATGGCTATGTCAGTATTTG
GGATGGTTTCAACAAAAAACGATTGTGTCAATTTCATCATTACGAAACTTCAATCTCTGC
AATTTCATTCAGTAATGATGGTAGTACGCTTGCTATTGGTTGCTCATATCTTGACGAATT
GGAAAAACCTCCGGAACCACTTCCCGAACCATCAATTTATGTACGTTATGTAAATGAACA
AGAAGTCAAACCCAAGTAA
>g2118.t2 Gene=g2118 Length=160
MANYVARRESSLKYQTRCIRVFPNKEGYVMSSIEGRVAVEYFDNDPEIQKKKFAFKCHRA
KENNIEMIYPVNAISFHNGFSTFATAGSDGYVSIWDGFNKKRLCQFHHYETSISAISFSN
DGSTLAIGCSYLDELEKPPEPLPEPSIYVRYVNEQEVKPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2118.t2 | Gene3D | G3DSA:2.130.10.10 | - | 2 | 160 | 0.0000 |
| 2 | g2118.t2 | PANTHER | PTHR10971 | MRNA EXPORT FACTOR AND BUB3 | 5 | 160 | 0.0000 |
| 3 | g2118.t2 | PANTHER | PTHR10971:SF31 | MITOTIC CHECKPOINT PROTEIN BUB3 | 5 | 160 | 0.0000 |
| 1 | g2118.t2 | Pfam | PF00400 | WD domain, G-beta repeat | 55 | 96 | 0.0150 |
| 7 | g2118.t2 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 64 | 128 | 8.7290 |
| 8 | g2118.t2 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 71 | 96 | 9.1720 |
| 5 | g2118.t2 | SMART | SM00320 | WD40_4 | 48 | 96 | 0.0023 |
| 4 | g2118.t2 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 13 | 130 | 0.0000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
