General information for g2124.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Head-specific guanylate cyclase.
Parent gene
- The parent gene g2124 is located on chr_3: 15338909..15341553 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2124 | g2124.t1 | isoform | g2124.t1 | 15338909 | 15341553 |
| chr_3 | g2124 | g2124.t1 | exon | g2124.t1.exon1 | 15338909 | 15339132 |
| chr_3 | g2124 | g2124.t1 | cds | g2124.t1.CDS1 | 15338909 | 15339132 |
| chr_3 | g2124 | g2124.t1 | exon | g2124.t1.exon2 | 15339189 | 15340126 |
| chr_3 | g2124 | g2124.t1 | cds | g2124.t1.CDS2 | 15339189 | 15340126 |
| chr_3 | g2124 | g2124.t1 | exon | g2124.t1.exon3 | 15340203 | 15340657 |
| chr_3 | g2124 | g2124.t1 | cds | g2124.t1.CDS3 | 15340203 | 15340657 |
| chr_3 | g2124 | g2124.t1 | exon | g2124.t1.exon4 | 15341140 | 15341294 |
| chr_3 | g2124 | g2124.t1 | cds | g2124.t1.CDS4 | 15341140 | 15341294 |
| chr_3 | g2124 | g2124.t1 | exon | g2124.t1.exon5 | 15341352 | 15341553 |
| chr_3 | g2124 | g2124.t1 | cds | g2124.t1.CDS5 | 15341352 | 15341553 |
| chr_3 | g2124 | g2124.t1 | TTS | g2124.t1 | 15341818 | 15341818 |
| chr_3 | g2124 | g2124.t1 | TSS | g2124.t1 | NA | NA |
Sequences
>g2124.t1 Gene=g2124 Length=1974
ATGACTTGTCCATTCGTACAAAGAAAGCCAGTCGATGCATTTCAACGACAACAGTCGACT
GTTGCAGATTTTGACTTACAAGACATTAACGACGATTCATTAAACCTGAAGCACATTAAC
GAAGCTTTGCAGCTTTTGACTGCACCAAGCAATCAGGCAATTAATCAGGCATTGCAATCT
CTCACTGAGAAATATGGCGAAAAATATCCTATTTTAGTAAAACTCGTTGAAGAGAAGAAT
AATATTTCATCGTTTCCAAATTATGACTATTTAGCTGACATACAAAATGCATTAATGGAA
GTCGATGAAGCGACAGTTGTTGATGTGTTGGTTTTATTGGGCGAAGAATTAATTGAGACA
TCATGCAAAGGAATTATCGAGAAAGCTTTTAAAAGTCTCGGTCAAAATATGAAAGAAATG
ATAGCATCCCTTGATGGTGTTTACGATGTATTGAAATTACAAGAAGAAGATTTAACTGAC
GCTGGTTTTATTGCGTCCGAGAATGAATTAATTTTCACATCAGATCGAATGTGTGTCGCT
TATGTATTACTTGGCATTTTGCAGCGTTTATCGAAGAAATTATATGGAGAACATAGTGTC
ATCACTATGGAAGTAATTGAGGGCAGCGTTCAAAAATTTCGTTATTTATTCAGCATAACT
GAAGAAGAGAAACCTGAAGCCCCAACACGTGAAATATCGTCTGATCCCAAAGATTTGTTG
ATGTCAAATAAGACTTACTGCAAAATGTTTCCTTGGCACTTTATTTTTGATGAAAGTCTA
GATTTAATCCAAGTCGGAGCTGCTTTTACAAAACTTTTCAAAAATTATTTGAAAACAACT
AAATCTGTTACAAATTTCTTTCGTTTTAAACGACCAGTTGGATTAAAACTTGAGTTTGAT
GAGATAGCAAAACGCACAAATACTCCATTTTTGCTTTCACTTCGTTCATCACGGCCAGAC
TTTGTTGCAAAAGGTTTGGAAATTAAGGGACAAATGGTTCTATGTAAGAACAGTAATCGA
AAAACTCTCATGTTTATTGGCTCACCTTCTTTAGATGGTTTAGAAGGACTGACATGCAAT
GGTCTCTTCTTATCAGATATTCCTATTTTTGATGCGACACGCGAAGTTATTTTAGTCGGA
GAACAAGCGAGAGCTCAAGATGGATTAAAGTTGCGCATGGCAAAATTGAAGAAAGAAGTT
GAAGAAGGGCATGAAGCGGTTTCTGAGGAACGAAAGAAAAATGTATCTTTATTGCAACTC
ATTTTTCCTGATGAAGTTGCAGAGAGATTGTGGCTTGGCAAGCCAGTTGATGCTCAAACA
TATGATAATGTAACAATGCTCTTTTCTGATATTGTTGGATTCACTTCTATTTGTTCCACC
GCAACTCCATTACAAGTTATTTCTATGCTTGAGAGTCTTTACAAAGTTTTTGATGAATAT
TGTGGAATTTTTGATGTCTATAAAGTAGAAACAATTGGTGATGCTTATTGTGTTGCAAAA
GGATTGGAAAGAAATAGCAAAACAATGCAATATGATGCATCAAAAGTTGCTTTTATGGCA
ATGAAAATGATTGAAACTTGTTCCAAGCATGTCACACATTCTGGAAAAAATATTCAGATG
AGAATTGGCTGCCACACCGCTACAGTTTTGAGTGGAGTAGTAGGAAGAAAAATGCCAAGA
TTTTGTTTAATGGGTCATCATGTCACAATCGCAAATAAATTTGAATCTGGAAGTGAAGCA
GGAAAAATTAATATTTCGAGTACAACTAAAAAAGCATTGGAATTGATTCCTGAATTTAAA
TTCGAATTTACTGAACGTGATTCATCATTTCTGCCAAAGGAATATTCAGCAGCACCAAAT
GAAACTTGTTACTTTTTGGAAGGATTTAAACACACATTAGTTGATGCAAATTCTGATATA
AGCATTCATATTGATGCTGCTATTAAGCAGATTAATTCTGAATCAAAAAACTAA
>g2124.t1 Gene=g2124 Length=657
MTCPFVQRKPVDAFQRQQSTVADFDLQDINDDSLNLKHINEALQLLTAPSNQAINQALQS
LTEKYGEKYPILVKLVEEKNNISSFPNYDYLADIQNALMEVDEATVVDVLVLLGEELIET
SCKGIIEKAFKSLGQNMKEMIASLDGVYDVLKLQEEDLTDAGFIASENELIFTSDRMCVA
YVLLGILQRLSKKLYGEHSVITMEVIEGSVQKFRYLFSITEEEKPEAPTREISSDPKDLL
MSNKTYCKMFPWHFIFDESLDLIQVGAAFTKLFKNYLKTTKSVTNFFRFKRPVGLKLEFD
EIAKRTNTPFLLSLRSSRPDFVAKGLEIKGQMVLCKNSNRKTLMFIGSPSLDGLEGLTCN
GLFLSDIPIFDATREVILVGEQARAQDGLKLRMAKLKKEVEEGHEAVSEERKKNVSLLQL
IFPDEVAERLWLGKPVDAQTYDNVTMLFSDIVGFTSICSTATPLQVISMLESLYKVFDEY
CGIFDVYKVETIGDAYCVAKGLERNSKTMQYDASKVAFMAMKMIETCSKHVTHSGKNIQM
RIGCHTATVLSGVVGRKMPRFCLMGHHVTIANKFESGSEAGKINISSTTKKALELIPEFK
FEFTERDSSFLPKEYSAAPNETCYFLEGFKHTLVDANSDISIHIDAAIKQINSESKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g2124.t1 | CDD | cd07302 | CHD | 443 | 608 | 1.20763E-41 |
| 11 | g2124.t1 | Coils | Coil | Coil | 393 | 413 | - |
| 10 | g2124.t1 | Gene3D | G3DSA:3.90.1520.10 | - | 52 | 226 | 2.6E-6 |
| 9 | g2124.t1 | Gene3D | G3DSA:3.30.450.260 | - | 241 | 367 | 7.6E-35 |
| 8 | g2124.t1 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 432 | 631 | 2.0E-62 |
| 5 | g2124.t1 | PANTHER | PTHR45655:SF6 | HEAD-SPECIFIC GUANYLATE CYCLASE | 94 | 656 | 2.1E-199 |
| 6 | g2124.t1 | PANTHER | PTHR45655 | GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2 | 94 | 656 | 2.1E-199 |
| 1 | g2124.t1 | Pfam | PF07700 | Haem-NO-binding | 79 | 201 | 5.3E-8 |
| 3 | g2124.t1 | Pfam | PF07701 | Heme NO binding associated | 241 | 317 | 9.6E-13 |
| 2 | g2124.t1 | Pfam | PF07701 | Heme NO binding associated | 324 | 430 | 3.0E-22 |
| 4 | g2124.t1 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 437 | 627 | 1.3E-53 |
| 14 | g2124.t1 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 445 | 575 | 36.429 |
| 13 | g2124.t1 | SMART | SM00044 | cyc_6 | 409 | 603 | 2.3E-73 |
| 7 | g2124.t1 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 431 | 604 | 1.07E-43 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006182 | cGMP biosynthetic process | BP |
| GO:0020037 | heme binding | MF |
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0035556 | intracellular signal transduction | BP |
| GO:0004383 | guanylate cyclase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K12318 (guanylate cyclase soluble subunit alpha)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
