General information for g2128.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dual specificity mitogen-activated protein kinase kinase dSOR1.
Parent gene
- The parent gene g2128 is located on chr_3: 15354894..15356042 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2128 | g2128.t1 | TSS | g2128.t1 | 15354050 | 15354050 |
| chr_3 | g2128 | g2128.t1 | isoform | g2128.t1 | 15354188 | 15355583 |
| chr_3 | g2128 | g2128.t1 | exon | g2128.t1.exon1 | 15354188 | 15354267 |
| chr_3 | g2128 | g2128.t1 | cds | g2128.t1.CDS1 | 15354188 | 15354267 |
| chr_3 | g2128 | g2128.t1 | exon | g2128.t1.exon2 | 15354335 | 15354530 |
| chr_3 | g2128 | g2128.t1 | cds | g2128.t1.CDS2 | 15354335 | 15354530 |
| chr_3 | g2128 | g2128.t1 | exon | g2128.t1.exon3 | 15354588 | 15354767 |
| chr_3 | g2128 | g2128.t1 | cds | g2128.t1.CDS3 | 15354588 | 15354767 |
| chr_3 | g2128 | g2128.t1 | exon | g2128.t1.exon4 | 15354826 | 15355416 |
| chr_3 | g2128 | g2128.t1 | cds | g2128.t1.CDS4 | 15354826 | 15355416 |
| chr_3 | g2128 | g2128.t1 | exon | g2128.t1.exon5 | 15355476 | 15355583 |
| chr_3 | g2128 | g2128.t1 | cds | g2128.t1.CDS5 | 15355476 | 15355583 |
| chr_3 | g2128 | g2128.t1 | TTS | g2128.t1 | 15355983 | 15355983 |
Sequences
>g2128.t1 Gene=g2128 Length=1155
ATGTCTAAGAACAAGCTAAATTTGACATTGCCTCCGCCTTCTATCGATGCTTCGATCACT
CCAGCTTTAGCTTCTGCAGAGAAAAAAGAATTACTTGGTACAAAAAATAGTATTGATCAA
TTAACAGAGACTTTAGAAGAACTCGATATGGATGAGAATGCACGCAAAAGGATAAAAGTA
TTTTTAAATCAAAAAGAGAAAATTGGTGAACTCTCAGATCAAGATCTTGAAAAATTAGGA
GAGTTAGGTTCTGGAAATGGAGGTGTAGTGATGATGGCACGACACAAACCAACTTCTCTC
ATAATGGCAAGAAAAATGATTCATCTTGAAGTAAAGCCAGCTATTAAGAAACAAATTATT
CGTGAATTGAAAGTATTGCATGAATGTAATTTTCCTCATATTGTTGGTTTCTATGGAGCC
TTTTATAGTGATGGCGAAATCAGCATTTGCATGGAGTATATGGACGGTGGTTCACTTGAT
TTGATTTTAAAACGTGCTGGACGTATACATGAGAACATTTTGGGCAAAATAACAGTTGCT
GTGTTAAAAGGTTTAACTTATTTACGTGATAAACATGCCATCATTCATAGAGATGTCAAA
CCGAGTAATATTTTGGTTAATAGCAGTGGAGAAATAAAAATTTGTGATTTTGGTGTAAGT
GGTCAATTGATTGATTCAATGGCAAACTCATTTGTCGGCACACGTAGCTATATGTCACCT
GAACGTTTACAAGGAACACATTATTCAGTTCAATCAGACATTTGGTCCTTAGGCCTCTCA
TTAGTGGAGATGGCTATAGGAATGTATCCAATTCCACCACCTGACATGCAGACACTTGAT
GCTATTCTCGCAAACGATGCACAATCATTAGCAAATCAGACAATTATTGAGCCACGATCG
ATGGCAATTTTTGAATTATTAGACTATATTGTGAATGAGCCACCACCGAAACTAGAGCAT
CCTAGTTTCTCGCCCAATTTCAAAGACTTTGTAGATAAATGTTTGAAGAAAAATCCTGAA
GAACGTGCTGATTTAAAGACATTAATGGCTCATGATTGGATAAGACATTCTGAAGAAGAA
AATGTTGATGTAGCTGGTTGGGTTTGTCGAACTATGAATTTGCCTCTTACTCCAAAACGC
ACGATACCACAGTAA
>g2128.t1 Gene=g2128 Length=384
MSKNKLNLTLPPPSIDASITPALASAEKKELLGTKNSIDQLTETLEELDMDENARKRIKV
FLNQKEKIGELSDQDLEKLGELGSGNGGVVMMARHKPTSLIMARKMIHLEVKPAIKKQII
RELKVLHECNFPHIVGFYGAFYSDGEISICMEYMDGGSLDLILKRAGRIHENILGKITVA
VLKGLTYLRDKHAIIHRDVKPSNILVNSSGEIKICDFGVSGQLIDSMANSFVGTRSYMSP
ERLQGTHYSVQSDIWSLGLSLVEMAIGMYPIPPPDMQTLDAILANDAQSLANQTIIEPRS
MAIFELLDYIVNEPPPKLEHPSFSPNFKDFVDKCLKKNPEERADLKTLMAHDWIRHSEEE
NVDVAGWVCRTMNLPLTPKRTIPQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g2128.t1 | CDD | cd06615 | PKc_MEK | 75 | 372 | 0.0 |
| 7 | g2128.t1 | Coils | Coil | Coil | 31 | 51 | - |
| 5 | g2128.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 42 | 155 | 9.7E-46 |
| 6 | g2128.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 156 | 379 | 1.1E-66 |
| 2 | g2128.t1 | PANTHER | PTHR48013:SF19 | DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1 | 1 | 375 | 4.3E-189 |
| 3 | g2128.t1 | PANTHER | PTHR48013 | DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED | 1 | 375 | 4.3E-189 |
| 1 | g2128.t1 | Pfam | PF00069 | Protein kinase domain | 77 | 354 | 4.6E-67 |
| 10 | g2128.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 82 | 105 | - |
| 9 | g2128.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 194 | 206 | - |
| 12 | g2128.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 76 | 354 | 45.785 |
| 11 | g2128.t1 | SMART | SM00220 | serkin_6 | 76 | 354 | 4.5E-83 |
| 4 | g2128.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 70 | 358 | 6.34E-79 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04368 (mitogen-activated protein kinase kinase 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
