General information for g2141.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as mRNA cap guanine-N7 methyltransferase.
Parent gene
- The parent gene g2141 is located on chr_3: 15567005..15567827 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2141 | g2141.t1 | TTS | g2141.t1 | 15566287 | 15566287 |
| chr_3 | g2141 | g2141.t1 | isoform | g2141.t1 | 15566457 | 15567827 |
| chr_3 | g2141 | g2141.t1 | exon | g2141.t1.exon1 | 15566457 | 15566506 |
| chr_3 | g2141 | g2141.t1 | cds | g2141.t1.CDS1 | 15566457 | 15566506 |
| chr_3 | g2141 | g2141.t1 | exon | g2141.t1.exon2 | 15566567 | 15567150 |
| chr_3 | g2141 | g2141.t1 | cds | g2141.t1.CDS2 | 15566567 | 15567150 |
| chr_3 | g2141 | g2141.t1 | exon | g2141.t1.exon3 | 15567207 | 15567488 |
| chr_3 | g2141 | g2141.t1 | cds | g2141.t1.CDS3 | 15567207 | 15567488 |
| chr_3 | g2141 | g2141.t1 | exon | g2141.t1.exon4 | 15567550 | 15567827 |
| chr_3 | g2141 | g2141.t1 | cds | g2141.t1.CDS4 | 15567550 | 15567827 |
| chr_3 | g2141 | g2141.t1 | TSS | g2141.t1 | 15567895 | 15567895 |
Sequences
>g2141.t1 Gene=g2141 Length=1194
ATGGAAGAATCTATTGTTGCAAGTGATGAAGAATATTTGACAGAAGAGGAAGAACAGCCA
GAACAGAGAGGAATTAAGCGAAAACATGAAGATGACGAAGACGACGAAAGAGAGCAAATA
AATCAAGATGATGAGCAAGAAGGCGGCACTCAAAGTACGAGTCACGATAATTCAATAGTG
GTTGCAAATCATTATAATAAATTACAAGAGAAAGGATTGGCTGAAAGATTTAATTCGAAA
ATTTTCTATTTGAGAAATTTTAATAATTGGATAAAGAGTATGCTTATAAGCGATTATCTC
GAGAAAATTAAAGATTCTATGCAACATGGTTCTCCTTTAAGAGTGTTAGATATGTGTTGT
GGTAAAGGTGGTGATTTGTTAAAGTGGCATAAAGGAAGAATTACTTATTTAATCTGTACT
GATATTGCTGAGATTAGTATAGAACAGTGTCGTGCACGTTATGAAAAAATTACAGCTAAT
GATCCACCTGAACGTAAATCATTTCAAGCAGAATTTTTCACGGCTGATTCAACAAGAGAT
CGACTTAGGGAACGATATATAGACCCTTCAATAGAACTGAATTTAGTTAGTTGTCAGTTT
GCTTTTCATTATTGTTTCGAATCACCAAAACAAGCAGAGCAAATGCTCAAGAATGTCTCA
GAATGTTTGAAAATAGGTGGTTATTTTATTGGCACTATACCTGATGCAAAAGACATCATG
AAGCGTCAACGTGCTGCACAATCAGATTCTTTTGGAAATGATGTTTTTAATATAAAATTT
TTGTCAGACCCAGATGATCTCCCAATATTTGGGGCAAAATATAATTTTCATTTAGATGGT
GTTGTTGATTGTCCAGAGTTTCTTGTCTATTTTCCCGCGTTTGTTAAACTTGCTAAACGT
TATAATTTAAAACTTATTGAGAATGTTCGGTTTGAAGATTATTTTCAAATGAAAATTGGG
AAGGGAAGGGGGTTGATTGAAAAAATGCAAGCTGCAGAACGATTTAGTCTTAATGGTAAT
CGTAATTTATCAAGGTCTAATGAAGAAGAATATAAACATGCAAAAGATTTTCTACTTCAA
AAAGGTGGATATGAAAATCGACGTAAATGTGGAACGCTTTCTGCATCTGAATGGGAAGCA
GCATCTCTATATATGACATTTGCCTTTCAAAAGGTAAAAGGACCAATTGAATGA
>g2141.t1 Gene=g2141 Length=397
MEESIVASDEEYLTEEEEQPEQRGIKRKHEDDEDDEREQINQDDEQEGGTQSTSHDNSIV
VANHYNKLQEKGLAERFNSKIFYLRNFNNWIKSMLISDYLEKIKDSMQHGSPLRVLDMCC
GKGGDLLKWHKGRITYLICTDIAEISIEQCRARYEKITANDPPERKSFQAEFFTADSTRD
RLRERYIDPSIELNLVSCQFAFHYCFESPKQAEQMLKNVSECLKIGGYFIGTIPDAKDIM
KRQRAAQSDSFGNDVFNIKFLSDPDDLPIFGAKYNFHLDGVVDCPEFLVYFPAFVKLAKR
YNLKLIENVRFEDYFQMKIGKGRGLIEKMQAAERFSLNGNRNLSRSNEEEYKHAKDFLLQ
KGGYENRRKCGTLSASEWEAASLYMTFAFQKVKGPIE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g2141.t1 | CDD | cd02440 | AdoMet_MTases | 114 | 230 | 1.59963E-7 |
| 6 | g2141.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 58 | - |
| 7 | g2141.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 17 | - |
| 5 | g2141.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 18 | 35 | - |
| 2 | g2141.t1 | PANTHER | PTHR12189 | MRNA GUANINE-7- METHYLTRANSFERASE | 33 | 392 | 1.6E-105 |
| 3 | g2141.t1 | PANTHER | PTHR12189:SF2 | MRNA CAP GUANINE-N7 METHYLTRANSFERASE | 33 | 392 | 1.6E-105 |
| 9 | g2141.t1 | PIRSF | PIRSF028762 | ABD1 | 2 | 394 | 7.2E-130 |
| 1 | g2141.t1 | Pfam | PF03291 | mRNA capping enzyme | 50 | 392 | 6.2E-106 |
| 8 | g2141.t1 | ProSiteProfiles | PS51562 | mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56) domain profile. | 40 | 397 | 74.493 |
| 4 | g2141.t1 | SUPERFAMILY | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferases | 80 | 321 | 4.6E-28 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006370 | 7-methylguanosine mRNA capping | BP |
| GO:0004482 | mRNA (guanine-N7-)-methyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00565 (mRNA (guanine-N7-)-methyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
